Effects of plant growth-promoting rhizobacteria on organic tomato seedling production

Özaktan, Hatice; Malkoclu, M.C.; Tüzel, Yüksel; Özteki̇n, Gölgen Bahar; Yolageldi, L.

doi:10.17660/actahortic.2017.1164.8

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…The PGP activities of P. agglomerans strains have been investigated through different application modes and under diverse experimental conditions (e.g., glasshouse, field, pots, greenhouse, etc. ; Nunes et al, 2002 ; Feng et al, 2006 ; Venkadesaperumal et al, 2014 ; Moustaine et al, 2016 ; Paredes-Páliz et al, 2016 ; Kamran et al, 2017 ; Ozaktan et al, 2017 ; Xie et al, 2017 ; Saia et al, 2020 ; Luziatelli et al, 2020a , b ).…”

Section: Introductionmentioning

confidence: 99%

Optimization of the growth conditions through response surface methodology and metabolomics for maximizing the auxin production by Pantoea agglomerans C1

Melini

Luziatelli²,

Bonini³

et al. 2023

Front. Microbiol.

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IntroductionThe fermentative production of auxin/indole 3-acetate (IAA) using selected Pantoea agglomerans strains can be a promising approach to developing novel plant biostimulants for agriculture use.MethodsBy integrating metabolomics and fermentation technologies, this study aimed to define the optimal culture conditions to obtain auxin/IAA-enriched plant postbiotics using P. agglomerans strain C1. Metabolomics analysis allowed us to demonstrate that the production of a selected.Results and discussion:Array of compounds with plant growth-promoting- (IAA and hypoxanthine) and biocontrol activity (NS-5, cyclohexanone, homo-L-arginine, methyl hexadecenoic acid, and indole-3-carbinol) can be stimulated by cultivating this strain on minimal saline medium amended with sucrose as a carbon source. We applied a three-level-two-factor central composite design (CCD) based response surface methodology (RSM) to explore the impact of the independent variables (rotation speed and medium liquid-to-flask volume ratio) on the production of IAA and IAA precursors. The ANOVA component of the CCD indicated that all the process-independent variables investigated significantly impacted the auxin/IAA production by P. agglomerans strain C1. The optimum values of variables were a rotation speed of 180 rpm and a medium liquid-to-flask volume ratio of 1:10. Using the CCD-RSM method, we obtained a maximum indole auxin production of 208.3 ± 0.4 mg IAAequ/L, which was a 40% increase compared to the growth conditions used in previous studies. Targeted metabolomics allowed us to demonstrate that the IAA product selectivity and the accumulation of the IAA precursor indole-3-pyruvic acid were significantly affected by the increase in the rotation speed and the aeration efficiency.

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