Effects of platelet-derived growth factor-AA on the healing process of tympanic membrane perforation

Yeo, Sang Won; Kim, Soo-Whan; Suh, Byung-Do; Cho, Seung-Ho

doi:10.1016/s0196-0709(00)85017-6

Cited by 32 publications

(26 citation statements)

References 16 publications

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“…In Tierstudien konnte bereits gezeigt werden, dass z. B. der epidermale Wachstumsfaktor ("epidermal growth factor", EGF) sowie der basische Fibroblastenwachstumsfaktor ("basic fibroblast growth factor", bFGF) zu einer beschleunigten Heilung akuter Perforationen [5,25,35] führen. Da frische traumatische Perforationen in der Regel spontan und schnell heilen [21,24], sind die bei ihnen gewonnenen Erkenntnisse jedoch nur unzureichend auf die Bedingungen bei einer chronischen Perforation übertragbar.…”

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Ein verbessertes Tiermodell f�r chronische Trommelfellperforationen

Kaftan

Hosemann

Beule

et al. 2004

HNO

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Ein verbessertes Tiermodell f�r chronische Trommelfellperforationen

Kaftan

Hosemann

Beule

et al. 2004

HNO

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“…5,[11][12][13][14] Other emerging regenerative methods for the potential treatment of chronic TM perforations either demonstrate poor efficacy or still need to be evaluated in terms of functional outcomes. [15][16][17][18][19][20][21][22][23][24][25] In vivo testing of hearing function in animal models relies on the ABR and DPOAE. [26][27][28] Auditory evoked potentials in mice are thought to be similar to that in humans, but reliance of measurement requires stability of a number of factors in …”

Section: Discussionmentioning

confidence: 99%

Functional Outcomes of Heparin-Binding Epidermal Growth Factor-Like Growth Factor for Regeneration of Chronic Tympanic Membrane Perforations in Mice

Maria

Gottlieb

Maria

et al. 2017

Tissue Engineering Part A

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We aim to demonstrate that regeneration of chronic tympanic perforations with heparin-binding epidermal growth factor-like growth factor (HB-EGF) delivered by an injectable hydrogel restored hearing to levels similar to that of nonperforated tympanic membranes. Chronic tympanic membrane perforation is currently managed as an outpatient surgery with tympanoplasty. Due to the costs of this procedure in the developed world and a lack of accessibility and resources in developing countries, there is a great need for a new treatment that does not require surgery. In this study, we show in a mouse model through measurement of auditory brainstem response and distortion product otoacoustic emissions that tympanic perforations lead to hearing loss and this can be predominantly recovered with HB-EGF treatment (5 mg/mL). Our animal model suggests a return to function between 2 and 6 months after treatment. Auditory brainstem response thresholds had returned to the control levels at 2 months, but the distortion product otoacoustic emissions returned between 2 and 6 months. We also show how the vibration characteristics of the regenerated tympanic membrane, as measured by laser Doppler vibrometry, can be similar to that of an unperforated tympanic membrane. Using the best available methods for preclinical evaluation in animal models, it is likely that HB-EGF-like growth factor treatment leads to regeneration of chronic tympanic membrane perforations and restoration of the tympanic membrane to normal function, suggesting a potential route for nonsurgical treatment.

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“…Treatment is surgical closure of the perforation under appropriate conditions in TM perforation. The treatment involves an invasive procedure requiring specialized equipment, resources and experience [12] . Thus, simple, non-invasive therapeutic modalities are needed that are less complicated.…”

Section: Discussionmentioning

confidence: 99%

“…In attempts to find a way for a better solution of this problem, experimental wound models on the tympanic membrane of rats have been applied and the results published. Previous studies have revealed the positive and negative effects of various agents on the healing of tympanic membranes, including hyaluronic acid, epidermal growth factor, transforming growth factor, platelet-derived growth factor, and platelet-rich plasma [10][11][12][13] . …”

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Potential Curative Role of Hypericum Perforatum in an Experimental Rat Model of Tympanic Membrane Perforation

Yaşar¹,

Kaya²,

Karaman³

et al. 2016

Int Adv Otol

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OBJECTIVE:Our aim was to investigate the curative role of topical Hypericum perforatum (HP) in a rat model of tympanic membrane perforation in a histopathological manner. MATERIALS AND METHODS:This study was conducted on 30 female Sprague-Dawley rats. An HP extract was prepared as a suspension in pure olive oil. In all rats, the right and left tympanic membranes were perforated by a fine puncture under a microscope. Rats were randomly assigned to three groups. The HP extract was given as drops on a daily basis in Group 1, while olive oil alone was given on a daily basis in Group 2. In Group 3, the rats were allowed spontaneous recovery without any intervention. Three rats from each group were sacrificed on days 7, 14, and 21. Temporal bullae were removed for histopathological examination to evaluate fibroblast proliferation, leukocyte infiltration, neovascularization, and subepithelial thickness and to compare these among the groups. RESULTS:In histopathological examination of the rats from each group on days 7, 14, and 21, there were significant differences in leukocyte infiltration, fibroblast proliferation, and subepithelial thickness. No significant difference was observed in neovascularization among the groups. CONCLUSION:Compared with the spontaneous recovery group, HP was found to be more effective in a wound-healing model of the tympanic membrane. HP may be applied in clinical practice if it is shown to be safe with regard to ototoxicity. comfortable and practical treatment protocol. In attempts to find a way for a better solution of this problem, experimental wound models on the tympanic membrane of rats have been applied and the results published. Previous studies have revealed the positive and negative effects of various agents on the healing of tympanic membranes, including hyaluronic acid, epidermal growth factor, transforming growth factor, platelet-derived growth factor, and platelet-rich plasma [10][11][12][13] . KEYWORDS:To the best of our knowledge, there are no data showing that HP has been used for healing tympanic membranes.Therefore, we aimed to assess the effect of HP olive oil extract on the wound-healing process in experimentally perforated tympanic membranes of rats. MATERIALS AND METHODS AnimalsThis study was conducted on 30 female Sprague-Dawley rats aged ≥6 weeks, weighing 200-300 g. The rats were fed with standard rat pellet and tap water ad libitum. Rats were housed in separate cages (five rats per cage) under standard conditions at a temperature of 21°C and maintaining a 12-hour dark-light cycle. This study was approved by the Ethics Committee on Animal Experiment and Research (2013-13/ 112). Experimental ProcedureHypericum perforatum was harvested from rural areas of Adana Province in July and then dried. In the laboratory, 50 g of dry HP was weighed. The weighed HP was immersed in 500 cc of olive oil in glass jars and left in the sunshine over 4 weeks until the occurrence of a red coloration. There is also a traditional recipe for this: "the content of the jar should be exposed ...

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Effects of platelet-derived growth factor-AA on the healing process of tympanic membrane perforation

Cited by 32 publications

References 16 publications

Ein verbessertes Tiermodell f�r chronische Trommelfellperforationen

Ein verbessertes Tiermodell f�r chronische Trommelfellperforationen

Functional Outcomes of Heparin-Binding Epidermal Growth Factor-Like Growth Factor for Regeneration of Chronic Tympanic Membrane Perforations in Mice

Potential Curative Role of Hypericum Perforatum in an Experimental Rat Model of Tympanic Membrane Perforation

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