Effects of platelet-rich plasma against experimental ischemia/reperfusion injury in rat testis

Sekerci, Cagri Akin; Tanıdır, Yılören; Sener, Te; Şener, Göksel; Çevik, Özge; Yarat, Ayşen; Alev, Burçin; Çetınel, Şule; Kervancioglu, Elif; Şahan, Ahmet; Akbal, Cem

doi:10.1016/j.jpurol.2016.12.016

Cited by 48 publications

(38 citation statements)

References 39 publications

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“…Increased caspase-3 mRNA expression in the control group was because physiological apoptosis occurs in the process of spermatogenesis (Said, 2004). The presence of caspase-3 in the control group was also in line with the study of Sekerci et al (2017), that involved giving PRP in the testis which experienced torsion. Morsy et al (2014) also found the same result, finding the higher presence of caspase-3 expression in the control group compared to the DM group.…”

Section: Caspase-3 Mrna Expression In Testicular Ratssupporting

confidence: 80%

“…The difference between this research and the research that states the success of PRP is the way of administering PRP, namely intraperitoneal injection in ovarian torsion (Bakacak et al, 2015) and intratesticular in testicular torsion (Sekerci et al, 2017). The method done by intratesticular injection is prone to autoimmune sperm because it damages the blood testis barrier.…”

Section: Igf-1 Mrna Expression In Testicular Ratsmentioning

confidence: 92%

“…But the results of this study are different from studies using PRP to repair damage to the ovaries and testes. Studies by Bakacak et al (2015) and Sekerci et al (2017) revealed that there was improvement in the provision of PRP.…”

Section: Igf-1 Mrna Expression In Testicular Ratsmentioning

confidence: 99%

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Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis

Istiqamah

Rizal

Puspitasari

2019

Indonesian J. Pharm.

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Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for complication of diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in diabetic rat testis. The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements. Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p=0.049 and significant decrease in IGF-1 mRNA expression with p=0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.

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Section: Caspase-3 Mrna Expression In Testicular Ratssupporting

confidence: 80%

Section: Igf-1 Mrna Expression In Testicular Ratsmentioning

confidence: 92%

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Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis

Istiqamah

Rizal

Puspitasari

2019

Indonesian J. Pharm.

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“…Hepatocyte growth factor was shown to ameliorate CKD; also via suppressing tubular epithelial to mesenchymal transition, apoptosis and inflammation [68] . Moreover, the adenosine produced by adenine nucleotides degradation could mediate tissue protection and repair by increasing oxygen supply, suppressing inflammation, and promoting angiogenesis [69] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Platelet Rich Plasma on an Experimental Rat Model of Adriamycin Induced Chronic Kidney Disease

Abd-Ellatif¹,

Atef

2019

The Medical Journal of Cairo University

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Background: Chronic kidney disease (CKD) is a worldwide health problem with increased mortality and morbidity. Currently, there is no effective protective therapy against CKD-induced renal damage with its sequel of end stage renal disease. Platelet-rich plasma (PRP) has a progressively gained consideration in wound healing, repair/regeneration of damaged tissues and conservation of organ function. Aim of Study: This current study was planned to evaluate the possible protective effect of PRP treatment on a rat model of Adriamycin (ADR)-induced CKD. Material and Methods: Sixty male albino rats were included; 30 were used for PRP preparation and the other 30, were randomly categorized into three equal groups of ten rat each: Normal control group, ADR group and PRP treated ADR group. At the end of experimental regimen (6 weeks), 24h urine samples were collected for measuring 24h urinary protein excretion and creatinine clearance (Cr Cl), then rats were decapitated and blood samples were collected for measuring serum albumin, triglycerides and cholesterol along with serum creatinine and blood urea nitrogen (BUN). Renal tissue samples were harvested and used for determination of renal level and mRNA expression of nephrin along with levels of hepatocyte growth factor (HGF), malondialdehyde (MDA), super oxide dismutase (SOD) activity, tumor necrosis factor alpha (TNF-α), interleukin 1-β (IL-1 β), and transforming growth factor 1 β (TGF 1 β) in renal homogenates. Results: Platelet rich plasma treatment improved ADR induced proteinuria, hyperlipidemia and renal dysfunction, which was accompanied by parallel upregulation of renal nephrin and inhanced levels of HGF and SOD activity, whereas the increased levels of TNF-α , IL-1 β and TGF 1 β were attenuated. Conclusion: Platelet rich plasma could potentially protect against ADR induced CKD by alleviating its associated proteinuria, hyperlipidemia and the altered oxidative stress, inflammatory and fibrogenic responses.

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“…Testis torsion (TT) is a case that is known worldwide as one of the reasons for male infertility which requires urgent surgical intervention. In TT, as a result of the spermatic cord turning vertically around its axis, blood flow is obstructed firstly by venous obstruction and then by stoppage of blood flow, and testes become ischaemic (Sekerci et al., ; Zhao, Lautz, Meeks, & Maizels, ). If TT is not immediately intervened with a surgical operation and treated, it may lead to the loss of the affected testis (Mellick, ) and a set of andrological issues, especially infertility, in connection with the ischaemia of germ cells (Kostakis et al., ; Ringdahl & Teague, ).…”

Section: Introductionmentioning

confidence: 99%

The ameliorative effects ofGinkgo bilobaon apoptosis, LH-R expression and sperm morphology anomaly in testicular torsion and detorsion

et al. 2018

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Torsion/detorsion (T/D) induces testicular damages in both germinal epithelial and interstitial tissues. Ginkgo biloba extract (GbE) exerts antioxidant and free radical scavenger. We investigated the effect of GbE on testicular tissues, Leydig and sperm cells in rats injured with T/D. Twenty-eight Wistar albino rats were randomly assigned into four groups (Control, GbE, Treatment: T/D+GbE, T/D). T/D performed to the rats in torsion, treatment received GbE (50 mg/kg) 1 hr before T/D, GbE group received only GbE (50 mg/kg) and control was defined as sham group. After T/D, the testes along with epididymis were removed and processed. LH-R expression, apoptosis, sperm morphology and histopathological damage scores were determined for each group. Testicular T/D caused significant increases in apoptosis and sperm morphology anomaly, and a significant decrease in Johnsen's testicular biopsy scores, LH-R expression of Leydig cell and normal sperm cell count. GbE ameliorated testicular histopathology and caused significant increases in LH-R expression, normal sperm cell count in the treated and particularly GbE group. Consequently, GbE may prevent testicular injury and enhance Leydig and sperm cell activity following both T/D and normal situation owing to its antioxidant, anti-apoptotic, free radical scavenger and anti-inflammatory effects.

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Effects of platelet-rich plasma against experimental ischemia/reperfusion injury in rat testis

Cited by 48 publications

References 39 publications

Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis

Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis

Effect of Platelet Rich Plasma on an Experimental Rat Model of Adriamycin Induced Chronic Kidney Disease

The ameliorative effects ofGinkgo bilobaon apoptosis, LH-R expression and sperm morphology anomaly in testicular torsion and detorsion

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