Effects of Platelet-rich Plasma and Cell Coculture on Angiogenesis in Human Dental Pulp Stem Cells and Endothelial Progenitor Cells

Li, Xinzhu; Hou, Jin; Wu, Buling; Chen, Ting; Luo, Aoxiang

doi:10.1016/j.joen.2014.07.022

Cited by 47 publications

(38 citation statements)

References 25 publications

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“…It increases migration and tube formation in HUVECs [40]. In a co-culture of endothelial progenitor cells (EPCs) and dental pulp stem cells, PRP was shown to increase VEGF and PDGF secretion thereby promoting vasculogenesis and stimulating EPCs to form vessel-like structures [59]. PRP contains high levels of angiopoetin 1 (Ang1), which mediates angiogenesis.…”

Section: Effects Of Platelet Rich Plasma In Tissue Engineeringmentioning

confidence: 99%

Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

et al. 2016

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Platelet rich blood derivatives have been widely used in different fields of medicine and stem cell based tissue engineering. They represent natural cocktails of autologous growth factor, which could provide an alternative for recombinant protein based approaches. Platelet rich blood derivatives, such as platelet rich plasma, have consistently shown to potentiate stem cell proliferation, migration, and differentiation. Here, we review the spectrum of platelet rich blood derivatives, discuss their current applications in tissue engineering and regenerative medicine, reflect on their effect on stem cells, and highlight current translational challenges.

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Section: Effects Of Platelet Rich Plasma In Tissue Engineeringmentioning

confidence: 99%

Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

et al. 2016

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“…Several studies have shed light on the regeneration of vascularized pulp by culturing endothelial and/or stem cells on flat substrates 13, 14 , in three-dimensional (3D) scaffold matrices 13–21 , and in scaffold-less tissue constructs 22, 23 . In earlier noteworthy developments Sakai et al .…”

Section: Introductionmentioning

confidence: 99%

A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs

Athirasala

Lins

Tahayeri

et al. 2017

Sci Rep

109

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The requirement for immediate vascularization of engineered dental pulp poses a major hurdle towards successful implementation of pulp regeneration as an effective therapeutic strategy for root canal therapy, especially in adult teeth. Here, we demonstrate a novel strategy to engineer pre-vascularized, cell-laden hydrogel pulp-like tissue constructs in full-length root canals for dental pulp regeneration. We utilized gelatin methacryloyl (GelMA) hydrogels with tunable physical and mechanical properties to determine the microenvironmental conditions (microstructure, degradation, swelling and elastic modulus) that enhanced viability, spreading and proliferation of encapsulated odontoblast-like cells (OD21), and the formation of endothelial monolayers by endothelial colony forming cells (ECFCs). GelMA hydrogels with higher polymer concentration (15% w/v) and stiffness enhanced OD21 cell viability, spreading and proliferation, as well as endothelial cell spreading and monolayer formation. We then fabricated pre-vascularized, full-length, dental pulp-like tissue constructs by dispensing OD21 cell-laden GelMA hydrogel prepolymer in root canals of extracted teeth and fabricating 500 µm channels throughout the root canals. ECFCs seeded into the microchannels successfully formed monolayers and underwent angiogenic sprouting within 7 days in culture. In summary, the proposed approach is a simple and effective strategy for engineering of pre-vascularized dental pulp constructs offering potentially beneficial translational outcomes.

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“…Co-culture of human DPSCs and endothelial cells promotes vasculogenesis compared to single cultures of endothelial cells [ 150 ]. The co-culture of human umbilical vein endothelial cells (HUVECs) with DPSCs demonstrated an increase in odonto-/osteo-genic potential of DPSCs as well as the vasculogenic potential of HUVECs, furthermore, DPSCs contributed to longer stabilization of vessel-like structures generated by HUVECs [ 140 ].…”

Section: Vascular Tissue Regenerationmentioning

confidence: 99%

Dental Stem Cells in Oral, Maxillofacial and Craniofacial Regeneration

Khojasteh

Nazeman

Rad

2016

Stem Cell Biology and Regenerative Medicine

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Effects of Platelet-rich Plasma and Cell Coculture on Angiogenesis in Human Dental Pulp Stem Cells and Endothelial Progenitor Cells

Cited by 47 publications

References 25 publications

Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

A Novel Strategy to Engineer Pre-Vascularized Full-Length Dental Pulp-like Tissue Constructs

Dental Stem Cells in Oral, Maxillofacial and Craniofacial Regeneration

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