Effects of pluronic F68 micellization on the viability of neuronal cells in culture

Abstract

Triblock copolymers with surface-active properties, referred to as Pluronic, have shown potential medical applications such as drug delivery to selective targets in the human body. In particular, the transport of anti-inflammatory substances to the brain is required for illness treatment, thus the study of delivery agents that cross the blood-brain barrier is relevant. In this article we study the effects of the micelle formation on the morphologic and cytotoxic properties of Pluronic F68. We determinate the critical micellar concentration (CMC) by standard tensiometric and absorbance measurements, and also we analyze the morphology of polymers by atomic force microscopy. Our observations indicate that the morphological properties of F68 are drastically modified in the CMC range, as well as the ability to increase the viability of neuroblastoma cells maintained under culture conditions, as compared with nontreated cells. Our conclusions highlight the close correlation between morphological and physiochemical properties of Pluronic, which must be further understood in order to achieve highly controlled pharmacological uses.

Lipid Vesicle Preparation Using W/O/W Multiple Emulsions Via Solvent Evaporation: The Effect of Emulsifiers on the Entrapment Yield of Hydrophilic Materials

“…This shows that at high concentration, Pluronic® F68 molecules are most likely adsorbed and incorporated into the membranes of the lipid vesicles, thereby increasing their average size. This assertion is consistent with reports that Pluronic® F68 unimers and micelles have the ability to incorporate and translocate across cell membranes, with the immersion of the hydrophobic polypropylene oxide (PPO) moiety into the membrane hydrophobic areas, thereby altering the structure of the membrane (Samith et al, ).…”

Micellar and structural stability of nanoscale amphiphilic polymers: Implications for anti-atherosclerotic bioactivity

“…Specifically, the NPSA is at least 50% larger for M12P5 compared with the other four AMPs considered in this study. Overall, all AMPs exhibited very low CMC values (10 −7 – 10 −5 M) compared to current drug delivery systems under investigation [33, 34], suggesting their promise for drug delivery applications.…”

Formulasi Micellar Based Water Ekstrak Bunga Telang

“…Poloksamer terdiri beberapa tipe diantaranya poloksamer L61, P85, F127 dan poloksamer F68 [10] . Salah satu jenis poloksamer yang banyak digunakan dalam kosmetik sebagai pembentuk misel adalah poloksamer F68 [11] . Poloksamer F68 memiliki beberapa keuntungan yaitu konsentrasi misel kritis yang sangat rendah sehingga potensi toksisitas minimal, tidak mengiritasi, mudah larut dalam air, kemampuan solubilisasi yang tinggi, stabil secara termodinamika di dalam larutan, memiliki efek hidrasi dan pembersihan terhadap kotoran baik yang bersifat lipofil maupun hidrofil [11][12] [13] .…”