Triboelectric separation is a promising technology to separate fine powders. To enable triboelectric separation for its application in industry, the impact of the process and product parameters must be examined. In this study, with regards to different wall materials in the charging step (PTFE, POM, PE, PVC, and PMMA), the influence of the powder composition of a binary starch-protein mixture with a protein content of 15 wt.%, 30 wt.% and 45 wt.% was studied. By increasing the protein content in the feed, the separation selectivity increased. No dependency of the empirical triboelectric series was determined for all powder compositions. The variation in the protein content of the initial powder and turbulent flow profiles results in a variation in the contact number of particles calculated. An increase in the contact number of particles leads to an increase in the protein content separated on the cathode, whereas the protein content on the anode is only slightly affected. These findings underpin the assumption that particle-particle interaction plays a decisive role in triboelectric charging of fine powders.