Effects of polyelectrolyte complex (PEC) on human periodontal ligament fibroblast (HPLF) function. II. Enhancement of HPLF differentiation and aggregation on PEC by L-ascorbic acid and dexamethasone

Abstract

In addition to many types of extra cellular matrix (ECM) in vivo, cells are stimulated by many types of vitamins, hormones, growth factors, etc. In this paper the effects of L-ascorbic acid 2-phosphate (Asc-2P) and dexamethasone (Dex) on proliferation and differentiation of human periodontal ligament fibroblast (HPLF) using polyelectrolyte complex (PEC) as a matrix in vitro will be discussed. The PEC was composed of chitosan as a polycation, with carboxymethyl (CPEC) or sulfated chitin (SPEC). Asc-2P (0.2 mM) inhibited the growth of HPLF on CPEC, but promoted the growth on SPEC. Moreover, the aggregation of HPLF on CPEC was inhibited by Asc-2P, but that on SPEC was induced in the presence of Asc-2P and Dex. Although Asc-2P reduced an increase in alkaline phosphatase (ALPase) activity of HPLF on CPEC as well, it induced a twofold increase in ALPase activities on SPEC and TCD. Furthermore, in the medium containing Asc-2P and 100 mM of Dex, cell growth was inhibited, but ALPase activity was promoted on both SPEC and TCD to form many aggregates on SPEC. ALPase activity increased by twofold over that of HPLF cultured in the medium containing only Asc-2P. Therefore, it is suggested that the cell functions of HPLF are controlled by the combination of PEC and additives.

Chitosan aerosol inhalation alleviates lipopolysaccharide- induced pulmonary fibrosis in rats

“…In vivo, chitosan were shown to decrease fibroblast infiltration and fibrosis [19], reduct the quantity of ECM deposition and degrade collagen synthesis to an appropriate rate [20][21][22]. In addition, chitosan can also stimulate reepithelialization in experimental wounds [23].…”

The Basic Research on Physiological Property of Functionalized Hyaluronan (II): Effect of Sulfated Hyaluronan on Histamine Release from the Mast Cell

“…These facts agree with our previous report; the cell specific function, proliferation and differentiation of in vitro cultured cells using a polyelectrolyte complex as a matrix, could be controlled by a kind and quantity of anionic residues, carboxyl and/or sulfate groups, of anionic polysaccharides. [12,13,21] We found that S-HA had high immunological safety as well as HA and other natural polysaccharides, as a medical material. Moreover, by controlling D.S.…”

“…And, we have already cleared up that a kind and quantity of anionic groups, carboxylic (-COOH) or sulfuric acid (-SO 3 H) groups, controlled cell functions cultured on polyelectrolyte complex (PEC) in vitro. [12,13] Therefore, in this study, we synthesized sulfated HA (S-HA) with various degrees of substitution and high molecular weight, which size is not the same as natural sulfated polysaccharides, in order to give HA new biological functions.…”

“…Molecular weights of HAs were measured by a viscosity method [13,14] in 0.2M NaCl aqueous solution at 30 . Chondroitin sulfate type-C (Chs-C) and type-A (Chs-A), Trimethylamine-SO 3 complex, heparin (Hep), bovine serum albumin (BSA), HEPES and compound 48/80 were used without further purification.…”

Chitosan‐phosphorylated chitosan polyelectrolyte complex hydrogel as an osteoblast carrier

“…Recently, PECs have generated much interest in medical and drug fields for applications such as catalysts, binding of pharmaceutical products, isolation and fractionation of proteins, and isolation of nucleic acid 6, 7. There are several reports that PEC membranes have excellent biocompatibility, and can control periodontal ligament cells' and osteoblasts' adhesion, growth, and morphology 8–12…”