Effects of polyethylene microplastics on cell membranes: A combined study of experiments and molecular dynamics simulations

Neurotoxicity mechanism of aconitine in HT22 cells studied by microfluidic chip-mass spectrometry

“…LDH is a common glycolytic enzyme in cells. The level of LDH release is a key indicator of cell membrane integrity, which means LDH in extracellular medium increases significantly when the cell membrane structure is damaged [ 22 ]. Therefore, evaluation of LDH leakage was considered as a criterion to test cell membrane integrity in this study.…”

Oxygen-Generating Scaffolds for Cardiac Tissue Engineering Applications

“…The OGM4 and OGM5 exhibited a significant increase in the LDH activity from day 1 through day 35. LDH is an enzyme that is released when there is damage to the cell membrane as experienced during cytotoxicity. , Higher levels of LDH are indicative of a higher amount of damage to the cell membrane, which occurs when the cells experience cytotoxicity. Therefore, decreased LDH levels indicated by low absorbance readings are indicative of lower cytotoxicity.…”

Exposure to polyethylene microplastics alters immature gut microbiome in an infant in vitro gut model

“…Our in vitro approach can definitively help for a better mechanistic understanding of the interactions between ingested micro-or even nano-sized plastics, the gut microbiota and mucus, considering inter-individual specificities. Further studies are needed to better investigate the effects of different plastic types, surface properties and sizes [105][106][107][108] on the structure and functions of the gut microbiota. The potential of the Tm-ARCOL model should be also extended to the simulation of other specific at-risk populations, such as neonates or pathological situations associated with gut microbial dysbiosis, such as obesity in infants [109].…”