Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation

Preparation and antioxidant activities of polysaccharides obtained from abalone viscera by combination of enzymolysis and multiple separation methods

“…Carbohydrate contents showed a clear increase after processing. Compared with traditional sevage method, which might lead to decreased overall polysaccharide yield, despite increases the deproteinization efficiency (Song et al, 2018), the ultrafiltration methods presented here retained 82.11% of carbohydrate in enzymatic hydrolysate, the yield of CAVP reached 19.72%, and the polysaccharide content of CAVP reached 51.75%, which were higher than those of the previous studies, reporting approximately 6.6 to 6.8% polysaccharide yields and 34.53 to 46.27% polysaccharide contents from abalone byproducts through sevage, repeated freezing and thawing methods Zhang et al, 2018). The proposed method is easy for the industrial preparation of polysaccharides.…”

“…DPPH radical scavenging activity was determined according to a reported method (Zhang et al., 2018). Briefly, 2 mL of sample solution with different concentrations (1, 2, 3, 4, and 5 mg/mL) was added to 2 mL (0.2 mmol/L) DPPH ethanol solution in a 5 mL cuvette.…”

“…Therefore, it is of great importance to develop and utilize the natural antioxidant active substances. The hydrolysates from abalone viscera are mainly composed of polysaccharides, peptides, and other bioactive molecules (Sun et al, 2010), with various biological activities, including free radical scavenging, antioxidant, antibacterial, anti-tumor, and immunomodulatory activities (Hao et al, 2019;Suleria, Masci, Gobe, & Osborne, 2017;Sun et al,2010;Zhang et al,2018).These researches have provided direction for further study of the polysaccharides from abalone viscera (AVP). However, some limitations exist in these studies and could be further improved (Sun et al, 2010;Zhu et al, 2011).…”

“…Abalones are single‐shelled marine mollusks. Traditionally, only the foot muscles of abalone are used for human consumption, whereas viscera and gonad tissue accounting for approximately 15 to 25% of the total weight of abalone are mostly considered as byproducts and are discarded (Zhang et al., 2018). According to the latest statistics (China Fishery Bureau, 2020), the annual production of abalone in China has reached 180, 267 tons in 2019, suggesting the abandonment of more than 27,000 tons (based on 15% of discard rate) of viscera and gonad tissue every year.…”

Enzymatic Extraction, Purification, and Characterization of Polysaccharides fromPenthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

“…The contents of carbohydrate in crude PCPP and its purified fraction (PCPP-1a) were evaluated by the phenol-sulfuric acid assay using a series of concentrations of D-glucose as standards [31]. The content of sulfate radical was assessed using the reported way [32]. The uronic acid content of PCPP and PCPP-1a was measured by m-hydroxydiphenyl method using D-galacturonic acid as a standard [33].…”