Effects of polysaccharides isolated from Inonotus obliquus against hydrogen peroxide-induced oxidative damage in RINm5F pancreatic β-cells

Abstract

The purpose of the present study was to elucidate the cytoprotective effects of polysaccharides isolated from Inonotus obliquus. The polysaccharides were extracted from the fruiting body of I. obliquus (PFIO) and the liquid culture broth of I. obliquus (PLIO). The effects of PFIO and PLIO on hydrogen peroxide (H2O2)‑induced oxidative damage of RINm5F pancreatic β‑cells were comparatively investigated using an MTT assay, immunofluorescent staining, flow cytometry, and western blot analyses in vitro. The results of the present study demonstrated that treatment with PFIO and PLIO decreased DNA fragmentation and the rate of apoptosis. In addition, pretreatment of cells with PFIO and PLIO prior to H2O2 exposure resulted in increased insulin secretion and scavenging activity for intracellular reactive oxygen species, as compared with treatment with H2O2 alone. The results of the present study suggested that PFIO and PLIO may exert protective effects against H2O2‑induced oxidative stress via the regulation of mitogen‑activated protein kinases, nuclear factor‑κB and apoptotic proteins. Therefore, PFIO and PLIO may have potential merit as a medicinal food for the prevention of diabetes.

Antidiabetic activities of polysaccharides separated from Inonotus obliquus via the modulation of oxidative stress in mice with streptozotocin-induced diabetes

“…Via the regulation of mitogen-activated protein kinases, nuclear factor (NF)–κB, and apoptotic proteins, polysaccharides isolated from the fruiting body of I . obliquus inhibited the hydrogen peroxide–induced oxidative damage in RINm5F pancreatic β-cells [17]. The ethanol extract from the dry matter of a culture broth of I .…”

Chaga mushroom (Inonotus obliquus) polysaccharides exhibit genoprotective effects in UVB-exposed embryonic zebrafish (Danio rerio) through coordinated expression of DNA repair genes

“…The IOP monomers revealed by GC analysis comprised certain unique sugars, including arabinose, xylulose, ribose, rhamnose, and glucose ( Figure 7 ) that are known to be components of branched polysaccharides, such as β-glucan, which is reported to possess several health benefits [ 25 ]. β-glucan has been reported to have anti-diabetic, anti-proliferative properties, and anti-tumorigenic properties [ 26 ]. Such beneficial effects could be attributed to the ability of β-glucan to scavenge free radicals generated due to endogenous or exogenous agents, such as ionizing UVB radiation, thereby assisting the cells to repair DNA [ 27 ].…”

Molecular insights and cell cycle assessment upon exposure to Chaga (Inonotus obliquus) mushroom polysaccharides in zebrafish (Danio rerio)

“…The recent surge in medicinal mushrooms because of their unique inherent beneficial attributes such as anti-inflammatory, anti-tumor, anti-oxidant, and anti-diabetic properties has been noteworthy 10,14,17,18 . Chaga mushroom is one such mushroom that is considered to be one of the highest anti-oxidant compounds 19 and possess several such beneficial properties. In the present study, we showed that the hot water extract of the Chaga mushroom polysaccharides significantly reduced apoptosis and the generation of intracellular ROS that protected the cellular DNA from oxidative damage, in addition to enhancing the development of zebrafish.…”