Effects of polysaccharides' molecular structure on membrane fouling and the related mechanisms

Zeng, Bizhen; Pan, Zhenxiang; Shen, Liguo; Zhao, Dieling; Teng, Jiaheng; Hong, Huachang; Lin, Hongjun

doi:10.1016/j.scitotenv.2022.155579

Cited by 50 publications

(2 citation statements)

References 54 publications

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“…The physical and chemical characterizations of the EPS were performed using various analytical techniques as per the standard protocols given in the literature [ 22 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

Andrew

Jayaraman

2022

Polymers

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The use of natural polysaccharides as biomaterials is gaining importance in tissue engineering due to their inherent biocompatibility. In this direction, the present study aims to explore the structure and biocompatibility of the EPS produced by Virgibacillus dokdonensis VITP14. This marine bacterium produces 17.3 g/L of EPS at 96 h of fermentation. The EPS was purified using ion exchange and gel permeation chromatographic methods. The porous web-like structure and elemental composition (C, O, Na, Mg, P, S) of the EPS were inferred from SEM and EDX analysis. AFM analysis revealed spike-like lumps with a surface roughness of 84.85 nm. The zeta potential value of −10 mV indicates the anionic nature of the EPS. Initial molecular characterization showed that the EPS is a heteropolysaccharide composed of glucose (25.8%), ribose (18.6%), fructose (31.5%), and xylose (24%), which are the monosaccharide units in the HPLC analysis. The FTIR spectrum indicates the presence of functional groups/bonds typical of EPSs (O-H, C-H, C-O-H, C-O, S=O, and P=O). The polymer has an average molecular weight of 555 kDa. Further, NMR analysis revealed the monomer composition, the existence of two α- and six β-glycosidic linkages, and the branched repeating unit as → 1)[α-D-Xylp-(1 → 2)-α-D-Glcp-(1 → 6)-β-D-Glcp-(1 → 5)]-β-D-Frup-(2 → 2)[β-D-Xylp-(1 → 4)]-β-D-Xylp-(1 → 6)-β-D-Fruf-(2 → 4)-β-D-Ribp-(1 →. The EPS is thermally stable till 251.4 °C. X-ray diffraction analysis confirmed the semicrystalline (54.2%) nature of the EPS. Further, the EPS exhibits significant water solubility (76.5%), water-holding capacity (266.8%), emulsifying index (66.8%), hemocompatibility (erythrocyte protection > 87%), and cytocompatibility (cell viability > 80% on RAW264.7 and keratinocyte HaCaT cells) at higher concentrations and prolongs coagulation time in APTT and PT tests. Our research unveils the significant biocompatibility of VITP14 EPS for synthesizing a variety of biomaterials.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

Andrew

Jayaraman

2022

Polymers

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“…Operational details of these characterization items can refer to the literature. [38][39][40][41][42] Separation performance of the membranes…”

Section: Membrane Characterizationmentioning

confidence: 99%

Enhanced water permeability in nanofiltration membranes using 3D accordion-like MXene particles with random orientation of 2D nanochannels

Zhang

et al. 2022

J. Mater. Chem. A

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The Effect of Natural Polysaccharides in Treatment of Diabetic Nephropathy: A Review

Yang,

Chen,

Zhou

et al. 2024

Starch Stärke

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Diabetic nephropathy is a serious metabolic disease that is usually caused by diabetes mellitus and ends in chronic kidney disease and renal failure. Many drugs used to treat diabetic nephropathy have limitations and side effects. There is an urgent need to find safe and effective new drugs for the treatment of diabetic nephropathy. The polysaccharides from natural sources, including plants, fungi, and algae, have good protective effects on diabetic nephropathy without side effects. In this paper, the natural sources, chemical composition, and structural characteristics of polysaccharides are reviewed. In addition, the study summarizes the different mechanisms that polysaccharides improve diabetic nephropathy, such as antioxidative stress, regulation of inflammation, inhibition of apoptosis, and renal fibrosis. This review provides theoretical support for the protective effect of polysaccharides and reveals potential therapeutic methods for diabetic nephropathy.

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Effects of polysaccharides' molecular structure on membrane fouling and the related mechanisms

Cited by 50 publications

References 54 publications

Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

Enhanced water permeability in nanofiltration membranes using 3D accordion-like MXene particles with random orientation of 2D nanochannels

The Effect of Natural Polysaccharides in Treatment of Diabetic Nephropathy: A Review

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