Effects of polysaccharides on glycometabolism based on gut microbiota alteration

Fang, Qin; Hu, Jielun; Nie, Qixing; Nie, Shaoping

doi:10.1016/j.tifs.2019.08.015

Cited by 125 publications

(64 citation statements)

References 82 publications

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“…Based on metabolomics analyses, researchers have adopted intervention approaches for the treatment of obesity [38]. The consumption of an HFD can increase energy extraction and decrease the production of obesity-suppressing SCFAs, resulting in obesity and metabolic disorders [39]. In addition, negative correlations have been shown to occur between BA and some metabol-ites in multiple metabolic pathways.…”

Section: Metabolites and Metabolic Pathway Analysismentioning

confidence: 99%

Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice

et al. 2020

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Background: Obesity is a major problem worldwide and severely affects public safety. As a metabolite of gut microbiota, endogenous butyric acid participates in energy and material metabolism. Considering the serious side effects and weight regain associated with existing weight loss interventions, novel strategies are urgently needed for prevention and treatment of obesity. Results: In the present study, we engineered Bacillus subtilis SCK6 to exhibited enhanced butyric acid production. Compared to the original Bacillus subtilis SCK6 strain, the genetically modified BsS-RS06550 strain had higher butyric acid production. The mice were randomly divided into four groups: a normal diet (C) group, a high-fat diet (HFD) group, an HFD + Bacillus subtilis SCK6 (HS) group and an HFD + BsS-RS06550 (HE) group. The results showed BsS-RS06550 decreased the body weight, body weight gain, and food intake of HFD mice. BsS-RS06550 had beneficial effects on blood glucose, insulin resistance and hepatic biochemistry. After the 14-week of experiment, fecal samples were collected for nontargeted liquid chromatography-mass spectrometry analysis to identify and quantify significant changes in metabolites. Sixteen potentially significant metabolites were screened, and BsS-RS06550 was shown to potentially regulate disorders in glutathione, methionine, tyrosine, phenylalanine, and purine metabolism and secondary bile acid biosynthesis. Conclusions: In this study, we successfully engineered Bacillus subtilis SCK6 to have enhanced butyric acid production. The results of this work revealed that the genetically modified live bacterium BsS-RS06550 showed potential antiobesity effects, which may have been related to regulating the levels of metabolites associated with obesity. These results indicate that the use of BsS-RS06550 may be a promising strategy to attenuate obesity.

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Section: Metabolites and Metabolic Pathway Analysismentioning

confidence: 99%

Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice

et al. 2020

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“…The major products of microbiological fermentation are organic acids (OA), including short‐chain fatty acids (SCFAs), which provide energy for intestinal epithelial cells, promote their growth, and maintain the function of intestinal barriers (Sun, Cheng, et al., 2020). SCFAs also exhibit a diverse range of health advantages for the host, such as gut function, pathogen inhibition, regulation of the immune system, and protection against glycometabolism‐related diseases (Fang et al., 2019; Qi & Tester, 2020). Polysaccharides can enhance the integrity and function of intestinal barriers by promoting goblet cells to secret mucus in the intestine and increasing the expression of tight junction proteins, such as claudin‐1 and ZO‐1, to repair the damaged intestinal barrier.…”

Section: Polysaccharides and Gut Microbiotamentioning

confidence: 99%

“…The major products of microbiological fermentation are organic acids (OA), including short-chain fatty acids (SCFAs), which provide energy for intestinal epithelial cells, promote their growth, and maintain the function of intestinal barriers (Sun, Cheng, et al, 2020). SCFAs also exhibit a diverse range of health advantages for the host, such as gut function, pathogen inhibition, regulation of the immune system, and protection against glycometabolism-related diseases (Fang et al, 2019;Qi & Tester, 2020) (Han, Wang, et al, 2020).…”

Section: Regulation Effects Of Indigestible Polysaccharides On Gut mentioning

confidence: 99%

Regulation effects of indigestible dietary polysaccharides on intestinal microflora: An overview

Ahmed

Yao

et al. 2020

J. Food Biochem.

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The human intestinal contains rich and diverse microbiota that utilizes a variety of polysaccharides. The intestinal microflora extends the metabolic functions of the body, obtaining energy from indigestible dietary polysaccharides. It is not only a highly competitive environment but also a comprehensive collaboration for these polysaccharides, as the microbiota work to maximize the energy harvested from them through the intestine. Indigestible dietary polysaccharides help to manage colon health and host health by affecting the gut microbial population. These polysaccharides also influence the metabolic activity of the intestinal microbiota by stimulating the formation of SCFAs. Most of these metabolic activities affect host physiology because the epithelium absorbs secondary metabolites and end products or transports them to the liver, where they could exert other beneficial effects. This article reviews the carbohydrates existing in the human intestine, the regulating actions of indigestible polysaccharides on intestinal microflora, and the molecular basis of the degradation process of these polysaccharides. Practical applications Large deals of researches have shown that indigestible polysaccharides possess an outstanding regulation effect on the intestinal microflora, which indicates that indigestible polysaccharides have the potential to be used as prebiotics in the functional food and pharmaceutical industries. However, it is not clear how gut microbiota metabolizes these dietary polysaccharides, and how the resulting gut metabolites may further affect the intestinal microflora population and metabolism. This paper reviews the indigestible dietary polysaccharides existing in the human intestine, the regulation of polysaccharides on gut microbiota, and the molecular basis of the degradation process of these polysaccharides. This review helps to better understand the relationship between indigestible dietary polysaccharides and intestinal microflora, which will provide powerful evidence for the potential use of these polysaccharides as functional foods.

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“…As mentioned above, to investigate the changes in the intestinal ora caused by pesticide exposure were necessary in this work [30,31]. In the present study, 16S rRNA gene sequencing for microbiome analysis was used to detect the microbial communities and evaluate the effects of pesticide exposure on mouse gut ora.…”

Section: Effects Of Pesticide Exposure On the Diversity Of Gut Flora mentioning

confidence: 97%

Gut Flora Mediated Mouse Metabolic Health Risk Caused by Dietary Exposure of Acetamiprid and Tebuconazole

Jingkun¹,

Zhao²,

Xu³

et al. 2020

Preprint

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Background:Dietary pesticide residue is an important dietary inducement of metabolic syndrome. Results:Chronic inflammation, insulin resistance, obesity, and non-alcoholic fatty liver disease were induced to tested mice through long-term and low-level of acetamiprid and tebuconazole. On the basis of these phenotypes, the mouse gut flora with metabolites, host circulation metabolic profiling, and their interrelations were investigated, and host metabolic pathways were detected. Results showed that pesticide exposure differently altered the abundance of gut microbial species, such as high ratio of Firmicutes/Bacteroidetes and increased high lipopolysaccharide-production species. Correlation analysis between gut flora and its metabolic profiling further explained these changes and their associations. Under these influences, metabolic profiling of host serum and liver was performed, and metabolic disorders were characterized. The relationships between serum and gut flora were determined via their significantly different metabolites. Alterations to the metabolic pathways of liver were clarified to deeply explore the influences on host physiology. Host metabolic disorders were evidently released by fructooligosaccharide and fecal microbiota transplantation intervention, directly proving that gut flora is a vital medium in metabolic health risk caused by pesticide exposure.Conclusion:Dietary long-term and low-level pesticides threated metabolic health via affecting intestinal flora. Metabolism of intestinal flora and host were all stressed by the exposure, and their alterations were in close proximity. Dietary interventions mitigated metabolic diseases via improving disorder of intestinal flora. This work supplied theoretical bases and intervention approaches to body metabolic problems caused by pesticides exposure on the basis of gut flora.

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Effects of polysaccharides on glycometabolism based on gut microbiota alteration

Cited by 125 publications

References 82 publications

Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice

Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice

Regulation effects of indigestible dietary polysaccharides on intestinal microflora: An overview

Gut Flora Mediated Mouse Metabolic Health Risk Caused by Dietary Exposure of Acetamiprid and Tebuconazole

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