Effects of Polyvinyl Pyrrolidone and Activated Charcoal to Control Effect of Phenolic Oxidation on In Vitro Culture Establishment Stage of Micropropagation of Sugarcane (Saccharum Officinarum L.)

Shimelis, Dereje; Bantte, Kassahun; Feyissa, Tilaye

doi:10.4172/2329-8863.1000184

Cited by 8 publications

(8 citation statements)

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“…Therefore, the beneficial effects of PVP supplementation may be because of reducing browning and improving explant response in the PLB induction. The previous report indicated a low concentration (0.1-0.5 g L -1 ) of PVP reduced browning in sugar cane (Shimelis, Bantte, & Feyissa, 2015).…”

Section: Exp 1 Effects Of Tdz and Pvp On Plb Induction From Leaf Exmentioning

confidence: 90%

Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci

et al. 2018

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Phalaenopsis amabilis, commonly known in Indonesia as 'Anggrek Bulan' is one of the Orchidaceae species typically having large (7-12 cm), pure white flowers with yellow coloration and some red stripes on its labellum (Handoyo, 2010). Based on their perianth morphology, there are six types of Phal. amabilis, such as Taiwan, Sumatera, Irian Jaya, Java, Borneo, and Grandiflora types. The Phal. amabilis from Sumatera has smaller perianth than those from Java and Borneo while Phal. grandiflora from Borneo is characterized as having the largest flower and more than 5 cm petals (Ikedo, n.d.). As in many orchid species, illegal harvest, forest destruction, and climatic changes may contribute to the decline of their natural populations (Crain & Tremblay, 2014; Crain & Tremblay, 2017; Fay, 2018). Therefore, it is necessary to develop concerted efforts for ex situ conservation of endangered orchid species before it becomes extinct. The development of ex-situ conservation requires the use of tissue culture as the alternative technology for clonal propagation (Merritt, Hay, Swarts, Sommerville, & Dixon, 2014). Various tissues and organs have been used as explants to initiate propagation of Phalaenopsis, such as leaf (Balilashaki, Naderi, Kalantari,

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Section: Exp 1 Effects Of Tdz and Pvp On Plb Induction From Leaf Exmentioning

confidence: 90%

Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci

et al. 2018

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“…Activated charcoal absorbs both toxic phenolic compounds and growth regulators, so the amounts of activated charcoal should be reduced critically (Nayanakantha et al, 2010). In Saccharum officinarum L., medium MS supplemented with 0.2% and 0.3% PVP, 100% and 80% of explants had viable tissues respectively, while in medium containing 0.3% and 0.4% activated charcoal 46% and 40% of explants survived respectively (Shimelis et al, 2015). Activated charcoal made culture medium dark, and hence it didn't allow free radicals to form through oxidation of phenolic compounds (Nayanakantha et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of browning problem during the callogenesis of Spartium junceum L.

Taghizadeh

Dastjerdi

2021

Ornam. Hortic.

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During different phases of in vitro culture, plant tissues may be exposed to some stresses that never encounter in their natural habitats. The most significant stresses which interfere with in vitro culture are pathogenic contamination and browning disorder. Since browning sign is occurred during all phases of in vitro culture of Spartium junceum L., the present study was done preventing explants from browning during disinfection and callogenesis phases using exposure time of sterilants (ethanol 0, 30, 60 s and home bleach 0, 10, 15 min), antioxidant compounds (PVP 0.5%, Activated charcoal 0.1%, Curcumin 0.1%), Running water (30 and 60 min) plant growth regulators (2,4-D 0, 0.5, 1 and 2 mg L-1 and BA 0, 0.1 and 0.2 mg L-1), and by changing light/dark conditions was designed. The results showed that ethanol 70% (30 s) in combination with home bleach 20% (10 min) had the best effect in control contaminations and browning sign in nodal explants of S. junceum. The application of PVP 0.5% in medium was the best treatment to control of browning nodal explants in callus induction phase. The highest callus formation and the lowest explant browning were obtained on the medium supplemented with 0.5 mg L-1 2,4-D under the darkness condition. According to the results of this study, how disinfection methods, culture medium compositions and light conditions were effective on the browning and callogenesis of Spartium junceum L.

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“…e stated objectives and results of the various micropropagation studies have wide coverage and include virus cleaning and disease-free plantlet production, screening for salt-tolerant plants, in vitro micropropagation of medicinal and high-value crops, identification of better microtuber induction under various levels of sucrose, optimization of various concentrations of sterilants and plant growth regulators, and in vitro conservations [10][11][12][13][14][15][16][17][18][19][20], [21][22][23][24][25][26][27][28][29][30][31], [32][33][34][35][36][37][38][39][40][41][42], [43][44][45][46][47][48][49][50][51][52][53][54][55]. Other objectives include hormonal and media treatments to overcome hyperhydricity (vitrification) of micropropagated shoots…”

Section: Described Abovementioning

confidence: 99%

“…Plant micropropagation reports in Ethiopia should thus pay attention to these presumed controversies and contribute their share to address some of the existing controversies in order to expand the application of micropropagation with the desired repeatability and standards. Diverse plant species including cereals, oil crops, spices, medicinal plants, high-value crops, fruit plants, root crops, and endangered multipurpose shrubs were utilized in various forms of plant micropropagation in Ethiopia [10][11][12][13][14][15][16][17][18][19][20], [21][22][23][24][25][26][27][28][29][30][31], [32][33][34][35][36][37][38][39][40][41][42], [43][44][45][46][47][48][49][50][51][52][53][54][55]. Critical assessment of these studies would facilitate the development of repeata...…”

Section: Introductionmentioning

confidence: 99%

Critical Review on Plant Micropropagation of Ethiopian Plants Reported So Far: Existing Gaps, Required Standardization, and Future Research Direction

Birmeta

Safawo²,

Geleta

et al. 2022

Advances in Agriculture

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Plant micropropagation research in Ethiopia requires concerted efforts to meet desired levels of application for sustainable utilization of the country's diverse plant genetic resources. The purpose of the present review is to provide an update on the results of plant micropropagation conducted so far in Ethiopia. It assessed their strengths and identified gaps in order to standardize research methods and indicate future research directions. Two cereals, three oil crops, three spices, five medicinal plants, two high-value crops, six fruit plants, nine root crops, and one endangered multipurpose shrub were reviewed. The assessment of previously published research was carried out in terms of methods used in the selection of ex plants and their disinfestations, culture vessels, and media used with a variety of combinations and concentrations of plant growth regulators, macro- and micronutrient requirements, culture environments, and genetic stability of regenerated plantlets. Further assessments include the utilization of plant growth-promoting microbes and applications of “omics” research in order to establish standardized, efficient, and cost-effective micropropagation techniques. The findings of the assessments are summarized and current advances are highlighted, along with recommendations for future plant micropropagation studies in the country.

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Effects of Polyvinyl Pyrrolidone and Activated Charcoal to Control Effect of Phenolic Oxidation on In Vitro Culture Establishment Stage of Micropropagation of Sugarcane (Saccharum Officinarum L.)

Cited by 8 publications

References 0 publications

Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci

Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci

Inhibition of browning problem during the callogenesis of Spartium junceum L.

Critical Review on Plant Micropropagation of Ethiopian Plants Reported So Far: Existing Gaps, Required Standardization, and Future Research Direction

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