SummaryThe impact of supplementing bread with calcium caseinate was studied at substitution levels of 5%, 10% and 15%. The research assessed the baking properties, nutritional value, glycaemic response and antioxidant activity of the experimental bread samples. Addition led to a significant increase in protein content, reaching up to 22% of the total energy supply at 15% substitution. Essential amino acids, such as valine, leucine and isoleucine, more than doubled in concentration at the 15% substitution level. Furthermore, the incorporation of calcium caseinate improved the textural properties of the bread, significantly increasing the bread volume and reducing setback viscosity (P < 0.05). The predicted glycaemic response, as measured by the area under the glucose release curve, showed a notable reduction in sugar levels at 15% substitution. These results suggest that bread enriched with calcium caseinate can serve as a valuable protein source for the population, offering both enhanced nutritional benefits and favourable baking characteristics.