Effects of porcine IL-17B and IL-17E against intestinal pathogenic microorganism

Zhang, Shuxia; Wu, Li; Chen, Jiawei; Wei, Jiatian; Cai, Hao; Ma, Miaopeng; Zhao, Peijing; Ming, Feiping; Jia, Junhao; Li, Jiayi; Fan, Qin; Liang, Qiao‐Mei; Deng, Jinbo; Zeng, Min; Zhang, Linghua

doi:10.1016/j.molimm.2019.10.011

Cited by 5 publications

(6 citation statements)

References 38 publications

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“…Consistently, Zhang et al. found that IL-17 can express in IPEC-J2 cells ( 48 ). Furthermore, Yu et al.…”

Section: Discussionsupporting

confidence: 56%

“…As shown in Supplementary Figure S2A, we confirmed that IL-17A can be expressed in IPEC J2 cells (Supplementary Figure S2A). Consistently, Zhang et al found that IL-17 can express in IPEC-J2 cells (48). Furthermore, Yu et al found that TLR5-mediated IL-17C expression in IPEC-J2 enhances immune responses in the intestinal epithelium against ETEC infection (49).…”

Section: Discussionmentioning

confidence: 78%

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Lactobacillus reuteri 1 Enhances Intestinal Epithelial Barrier Function and Alleviates the Inflammatory Response Induced by Enterotoxigenic Escherichia coli K88 via Suppressing the MLCK Signaling Pathway in IPEC-J2 Cells

et al. 2022

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Intestinal epithelial barrier injury disrupts immune homeostasis and leads to many intestinal disorders. Lactobacillus reuteri (L. reuteri) strains can influence immune system development and intestinal function. However, the underlying mechanisms of L. reuteri LR1 that regulate inflammatory response and intestinal integrity are still unknown. The present study aimed to determine the effects of LR1 on the ETEC K88-induced intestinal epithelial injury on the inflammatory response, intestinal epithelial barrier function, and the MLCK signal pathway and its underlying mechanism. Here, we showed that the 1 × 109 cfu/ml LR1 treatment for 4 h dramatically decreased interleukin-8 (IL-8) and IL-6 expression. Then, the data indicated that the 1 × 108 cfu/ml ETEC K88 treatment for 4 h dramatically enhanced IL-8, IL-6, and tumor necrosis factor-α (TNF-α) expression. Furthermore, scanning electron microscope (SEM) data indicated that pretreatment with LR1 inhibited the ETEC K88 that adhered on IPEC-J2 and alleviated the scratch injury of IPEC J2 cells. Moreover, LR1 pretreatment significantly reversed the declined transepithelial electrical resistance (TER) and tight junction protein level, and enhanced the induction by ETEC K88 treatment. Additionally, LR1 pretreatment dramatically declined IL-8, IL-17A, IL-6, and TNF-α levels compared with the ETEC K88 group. Then, ETEC K88-treated IPEC-J2 cells had a higher level of myosin light-chain kinase (MLCK), higher MLC levels, and a lower Rho-associated kinase (ROCK) level than the control group, while LR1 pretreatment significantly declined the MLCK and MLC expression and enhanced ROCK level in the ETEC K88-challenged IPEC-J2 cells. Mechanistically, depletion of MLCK significantly declined MLC expression in IPEC-J2 challenged with ETEC K88 compared to the si NC+ETEC K88 group. On the other hand, the TER of the si MLCK+ETEC K88 group was higher and the FD4 flux in the si MLCK+ETEC K88 group was lower compared with the si NC+ETEC K88 group. In addition, depletion of MLCK significantly enhanced Claudin-1 level and declined IL-8 and TNF-α levels in IPEC-J2 pretreated with LR1 followed by challenging with ETEC K88. In conclusion, our work indicated that L. reuteri LR1 can decline inflammatory response and improve intestinal epithelial barrier function through suppressing the MLCK signal pathway in the ETEC K88-challenged IPEC-J2.

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“…Consistently, Zhang et al. found that IL-17 can express in IPEC-J2 cells ( 48 ). Furthermore, Yu et al.…”

Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 78%

Lactobacillus reuteri 1 Enhances Intestinal Epithelial Barrier Function and Alleviates the Inflammatory Response Induced by Enterotoxigenic Escherichia coli K88 via Suppressing the MLCK Signaling Pathway in IPEC-J2 Cells

et al. 2022

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“…Based on our previous studies, we have found that chicken lymphocytes were suitable for the preliminary evaluation of CpG-DNA [ 35 ]. Therefore, in this section, we stimulated chicken lymphocytes with pNZ8148-CpG10 and LACpG10-HL, respectively, to determine the expression level of genes related to innate immunity (IL-1β, IFN-γ, and IFN-α, Figure 1 A and LC3A, LC3B, Beclin 1, and p62, Figure 1 B) at different time points (6 h, 20 h, and 30 h).…”

Section: Resultsmentioning

confidence: 99%

“…In order to evaluate the effects of LACpG10-HL in infectious circumstances, Pseudomonas aeruginosa , Shigella sonnei , Enterohemorrhagic Escherichia coli (EHEC), and Streptococcus pyogenes [ 35 ] were used to challenge the cell models (HD11 and IPEC-J2), mimicking the natural surroundings.…”

Section: Resultsmentioning

confidence: 99%

“…In this section, we focused on the antibacterial immunoactivities of the stimulants in HD11 and IPEC-J2 cells infected by pathogens. Before adding pathogens, HD11 and IPEC-J2 cells were seeded into 24-plates at 1 × 10 5 cells/well without antibiotics [ 35 ]. When the cell adherence rate reached 75–85% after 8–12 h, the cells were pretreated respectively with PBS, pNZ8148 (10 μg/mL), pNZ8148-CpG10 (10 μg/mL), and LACpG10-HL solution (containing 10 μg/mL pNZ8148-CpG10) for 30 h. Meanwhile, the four pathogens described in Section 4.2 were cultured overnight, then collected and washed with PBS three times and adjusted to 1 × 10 8 CFU/mL in DMEM-F12 (1:1) (Gibico, Guangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

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LACpG10-HL Functions Effectively in Antibiotic-Free and Healthy Husbandry by Improving the Innate Immunity

Pan

Zhao

et al. 2022

IJMS

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Antibiotics are broadly restricted in modern husbandry farming, necessitating the need for efficient and low-cost immunomodulatory preparations in antibiotic-free and healthful farming. As is known to all, CpG oligonucleotides (CpG-ODNs, an effective innate immunostimulatory agent) recognized by TLR9 in mammals (while TLR21 in avians) could collaborate with some united agent to induce stronger immune responses, but the cost is prohibitively expensive for farmers. Here, considering the coordination between TLR2 and TLR9/TLR21, we firstly proposed the idea that the well-fermented Lactococcus lactis could be utilized as a CpG-plasmid carrier (LACpG10) to enhance the host's innate immunity against pathogenic invasion. In the present study, after obtaining LACpG10-HL from homogenized and lyophilized recombinant strain LACpG10, we treated primary chicken lymphocytes, two cell lines (HD11 and IPEC-J2), and chickens with LACpG10-HL, CpG plasmids (pNZ8148-CpG10), and other stimulants, and respectively confirmed the effects by conducting qRT-PCR, bacterial infection assays, and a zoological experiment. Our data showed that LACpG10-HL could induce excellent innate immunity by regulating autophagy reactions, cytokine expression, and motivating PRRs. Interestingly, despite having no direct antiseptic effect, LACpG10-HL improved the antibacterial capacities of lymphocytes and enterocytes at the first line of defense. Most importantly, water-supplied LACpG10-HL treatment reduced the average adverse event rates, demonstrating that LACpG10-HL maintained its excellent immunostimulatory and protective properties under farming conditions. Our research not only contributes to revealing the satisfactory effects of LACpG10-HL but also sheds new light on a cost-effective solution with optimal immune effects in green, antibiotic-free, and healthful husbandry farming.

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Protective effects of Clostridium butyricum against oxidative stress induced by food processing and lipid-derived aldehydes in Caco-2 cells

Shen

Zhao

et al. 2020

Appl Microbiol Biotechnol

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Effects of porcine IL-17B and IL-17E against intestinal pathogenic microorganism

Cited by 5 publications

References 38 publications

Lactobacillus reuteri 1 Enhances Intestinal Epithelial Barrier Function and Alleviates the Inflammatory Response Induced by Enterotoxigenic Escherichia coli K88 via Suppressing the MLCK Signaling Pathway in IPEC-J2 Cells

Lactobacillus reuteri 1 Enhances Intestinal Epithelial Barrier Function and Alleviates the Inflammatory Response Induced by Enterotoxigenic Escherichia coli K88 via Suppressing the MLCK Signaling Pathway in IPEC-J2 Cells

LACpG10-HL Functions Effectively in Antibiotic-Free and Healthy Husbandry by Improving the Innate Immunity

Protective effects of Clostridium butyricum against oxidative stress induced by food processing and lipid-derived aldehydes in Caco-2 cells

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