Effects of Portulaca Oleracea Extract on Acute Alcoholic Liver Injury of Rats

Abstract

The present study was envisaged to investigate the chemical constituents and the intervention effects of Portulaca oleracea extract (POE) on acute alcoholic liver injury of rats. The chemical composition of POE was detected by high performance liquid chromatography (HPLC). Sixty male Wistar rats were divided into 6 groups: Normal control (NC) group, acute alcoholic liver injury model group (ALI), low, medium and high dose of POE (25, 50, 100 mg/kg) groups and bifendate (BF, 3.75 mg/kg) group. Each group was given by intragastrical administration for 7 days. Alcoholic liver injury was induced in the experimental model by administering 50% ethanol at 8 mL/kg and repeated administration after 6 h, for a period of 7 days. The results showed that pretreatment with POE significantly reduced the ethanol-elevated serum level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and triglyceride (TG). The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in liver were enhanced followed by administration of POE, while the content of nitric oxide (NO) and malondialdehyde (MDA) was found to decrease. Hepatic content of tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) was also reduced by POE treatment. These results indicated that POE could increase the antioxidant capacity and relieve the inflammatory injury of the liver cells induced by ethanol. Meanwhile, in our study, POE reduced the expression of miR-122, acetyl coenzyme A carboxylase (ACC) 1 mRNA and protein and increased the expression of lipoprotein lipase (LPL) mRNA and protein in liver, which indicated that POE could improve the lipid metabolism disorder induced by ethanol. Our findings suggested that POE had protective effects on acute alcoholic liver injury of rats.

Isolation and Identification of Lactobacillus plantarum HFY05 from Natural Fermented Yak Yogurt and Its Effect on Alcoholic Liver Injury in Mice

“…IFN-γ is a pro-inflammatory cytokine that enhances the sensitivity of liver cells to TNF-α, making liver cells more easily damaged [44]. The oxidative stress state after liver injury causes an imbalance in inflammatory factors, including TNF-α and IL-6 in the body, leading to an increase in TNF-α and IL-6 in the liver [45]. LP-HFY05 reduced liver injury by regulating the levels of IL-6, IL-12, TNF-α, and IFN-γ in the serum of liver-injured mice, which was a similar effect as silymarin.…”

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“…The results showed that P. oleracea enhanced the antioxidant potential and relieved the liver cells from inflammatory injury which was induced by ethanol. P. oleracea extract treatment also decreased the expression of miR-122 47 . MiR-122 is micro RNA.…”

“…It is the second-largest liver disorder after viral hepatitis. Work was done to know the effectiveness of P. oleracea in treating the acute alcoholic liver injury in rats 47 . Flavonoids like hesperidin, myricetin, rutin and quercetin were detected in P. oleracea extract with the help of high performance liquid chromatography.…”

“…It has been reported that inhibition of miR-122 regulates the expression of genes involved in the synthesis and oxidation of lipids, resulting in a reduction in the levels of triglyceride and cholesterol and a significant improvement in liver steatosis 50,51 . Acetyl coenzyme A carboxylase 1 (ACC1) mRNA expression decreased, and lipoprotein lipase (LPL) mRNA expression increased when pretreatment of P. oleracea extract was given to the rats with acute alcoholic liver injury 47 . MiR -122 silencing inhibit the genes that influence lipid metabolism and transport of lipids, which are synthesized in the adipose tissue.…”

White Peony (Fermented Camellia sinensis) Polyphenols Help Prevent Alcoholic Liver Injury via Antioxidation

“…IFN-γ is a proinflammatory cytokine and can enhance the sensitivity of liver cells to TNF-α, rendering liver cells susceptible to damage [34]. Oxidative stress appearing after liver tissue injury can cause an imbalance in inflammatory factors, including IL-6, IL-12, TNF-α, and IFN-γ, in the body, thus increasing their contents in the liver [35]. WPPs can alleviate liver injury by regulating the serum levels of IL-6, IL-12, TNF-α, and IFN-γ in mice with liver injury, and the effect is similar to that of silymarin.…”