Effects of Povidone-Iodine Chemical Preparation and Saline Irrigation on the Perilimbal Flora

Boes, David A.; Lindquist, Thomas D.; Fritsche, Thomas R.; Kalina, Robert E.

doi:10.1016/s0161-6420(92)31765-8

Cited by 56 publications

(30 citation statements)

References 26 publications

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“…In fact, the current culture media for isolating conjunctival bacteria described in Chapter 1 21 is different from that in the Khorazo et al studies 3 mentioned in Chapter 24 of the same volume, in the same book 21 (revised 10 years before). In more recent studies [25][26][27][28][29][30][31][32][33][34][35][36][37][38] most samples of patients undergoing cataract surgery have a small size and the frequency of the isolated bacteria varies a lot between them due to different factors 18 (the isolating culture media used, the exclusion criteria used to select the patients and the geographical area where they lived). Among these factors the climatic conditions in Madrid have been statistically associated with the conjunctival bacteria frequency of our patients undergoing cataract surgery.…”

Section: Discussionmentioning

confidence: 99%

Influence of age on conjunctival bacteria of patients undergoing cataract surgery

Rubio

2005

Eye

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Aim To ascertain the effect of elderly in the conjunctival bacteria frequency of patients undergoing cataract extraction. Methods A retrospective case series study of 4432 consecutive patients who underwent cataract surgery, without excluding any of them for having pre-existing diseases. Their preoperative conjunctival culture were performed over a 3-year period (1994)(1995)(1996). Bacteria were grouped in nine categories and patients were divided into seven groups, according to age; comparisons between groups were made by means of the v 2 test, and the Mantel-Haenszel test to analyse age as a confounder, using SPSS program, version 12. Results Patients aged over 74 years accounted for 41.4%; women predominated among this group (61.4%), but not in the patients younger than 75 (47.7%); In the whole sample women accounted for 53.4%. Patients aged 75-96 years had a greater frequency of: Corynebacteria, Staphylococcus aureus, Streptococcus sp. (except Streptococcus pneumoniae), Gram-negative cocci and Gramnegative rods (except Haemophilus sp.) and 'other bacteria' categories than those aged 3-74 years. Different bacteria frequency in both sexes produced a confounding effect in the comparison between age-groups. Men had more Staphylococci coagulase (-), S. pneumoniae and Gram-negative rods than women. Conclusions Elderly patients awaiting cataract surgery had more conjunctival bacteria than those younger than 75 years, except Staphylococcus coagulase negative, S. pneumoniae and Haemophilus sp. At any age, men had more bacteria than women. These increments of frequencies could increase the risk of intraocular surgery contamination.

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Section: Discussionmentioning

confidence: 99%

Influence of age on conjunctival bacteria of patients undergoing cataract surgery

Rubio

2005

Eye

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“…When the conjunctival flora of patients submitted to facectomy was assessed prior to surgery, bacteria capable of causing endophtalmia were found in 74% of these individuals (45). On the other hand, it is well known that despite intense preoperative care of patients, either employing antibiotics or polyvinyl-pyrrolidone, the conjunctiva does not become refractory to bacterial growth (2,5,6,8,16). We demonstrated in this study that it may be possible to occur contamination of IOL if they touch the conjunctiva, mainly by staphylococci.…”

Section: Discussionmentioning

confidence: 99%

“…However, some studies have reported bacterial contamination of the aqueous humour during cataract surgery, without occurring post-operative ocular infection (3,37,43). Conjunctival endogenous microbiota is the main cause of postoperative and post-traumatic eye infections (2,5,8). Molecular techniques have shown a great similarity among strains isolated from vitreous humour, eye anterior chamber and intraocular lens (IOL) in patients with Coagulase-negative staphylococci endophtalmia (17).…”

Section: Introductionmentioning

confidence: 99%

Conjunctival endogenous microbiota in patients submitted to cataract surgery

Locatelli

Kwitko

Simonetti

et al. 2003

Braz. J. Microbiol.

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Bacterial isolation, identification and antimicrobial susceptibility tests were carried out in ocular material collected with swab and polimethylmethacrylate (PMMA) or silicone intraocular lenses (IOL) from forty six patients submitted to cataract surgery. Seventy six isolates and seven different microorganisms were identified. Coagulase-negative staphylococci (CNS) were the predominant microorganisms isolated from swabs (71.4% of cases), PMMA lenses (81.3%) and silicon lenses (77.8%). Coagulase-negative staphylococci isolates revealed high resistance to penicillin G followed by tetracycline, chloramphenicol and aminoglicosides. However, these isolates displayed great susceptibility to vancomycin, cephalothin and ofloxacin. Except for penicillin G, Staphylococcus aureus was very sensitive to the antimicrobial agents including oxacillin. Among Gram-negatives, Proteus mirabilis was prevalent and presented high resistance to tetracycline and chloramphenicol. Enterococcus isolates were vancomycin sensitive.

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“…A microbiota endógena conjuntival é a primeira suspeita quando se está pesquisando a causa de infecções oculares pós-traumáticas e pós-operatórias (4)(5)(6)(7)(8)(9) . Diferentes tipos de bactérias podem causar endoftalmite sendo estafilococos coagulase-negativos, mais especificamente S. epidermidis, os agentes mais comumente isolados (6,(10)(11)(12)(13) .…”

Section: Introductionunclassified

Aderência bacteriana in vitro a lentes intra-oculares de polimetilmetacrilato e de silicone

Locatelli

Englert

Kwitko

et al. 2004

Arq. Bras. Oftalmol.

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Objetivos: Verificar a aderência bacteriana a lentes intra-oculares de silicone e de polimetilmetacrilato como possível fator de risco no desenvolvimento de endoftalmite pós-operatória, utilizando-se um modelo in vitro com três microrganismos potencialmente patogênicos. Métodos: As análises foram realizadas com cepas de Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis (amostra clínica) e Pseudomonas aeruginosa ATCC 27853 incluindo a determinação de curvas de crescimento, testes para verificação de produção de cápsula, avaliação da hidrofobicidade, testes de aderência a diferentes materiais, microscopia óptica, microscopia eletrônica de varredura e microscopia de força atômica. Resultados: A produção de cápsula e a aderência das três diferentes cepas não mostraram qualquer relação com a quantidade de microrganismos; em relação às lentes intra-oculares de polimetilmetacrilato e de silicone, não houve diferença estatisticamente significativa na aderência de S. aureus e S. epidermidis; P. aeruginosa foi o microrganismo mais aderente a ambos os materiais. A microscopia eletrônica de varredura confirmou estes achados em relação à aderência, ao peso que a microscopia de força atômica evidenciou a produção de biofilme pelas cepas de S. aureus, S. epidermidis e P. aeruginosa. Conclusões: Constatou-se, in vitro, que os materiais analisados não diferiram com relação à taxa de aderência bacteriana, porém, P. aeruginosa apresentou maior eficiência de adesão entre as bactérias testadas. Todas as cepas produziram biofilme. Silicone foi o material mais hidrofóbico, quando comparado ao polimetilmetacrilato. INTRODUÇÃOCirurgia de catarata para implante de lentes intra-oculares (LIO) pode levar à endoftalmite pós-operatória e, ainda que raramente, à perda total da visão no olho acometido em até 50% dos casos (1)(2)(3) . A microbiota endógena conjuntival é a primeira suspeita quando se está pesquisando a causa de infecções oculares pós-traumáticas e pós-operatórias (4)(5)(6)(7)(8)(9) . Diferentes tipos de bactérias podem causar endoftalmite sendo estafilococos coagulase-negativos, mais especificamente S. epidermidis, os agentes mais comumente isolados (6,(10)(11)(12)(13) . Sabe-se que S. epidermidis tem habilidade de se aderir e proliferar em superfícies de polímeros, especialmente lentes intra-oculares, secretando uma matriz extracelular viscosa (biofilme) que o protege contra antibióticos, bem como dos mecanismos de defesa do hospedeiro (10,(14)(15)(16) . A adesão bacteriana a lentes intra-oculares e a produção de biofilme parecem

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Effects of Povidone-Iodine Chemical Preparation and Saline Irrigation on the Perilimbal Flora

Cited by 56 publications

References 26 publications

Influence of age on conjunctival bacteria of patients undergoing cataract surgery

Influence of age on conjunctival bacteria of patients undergoing cataract surgery

Conjunctival endogenous microbiota in patients submitted to cataract surgery

Aderência bacteriana in vitro a lentes intra-oculares de polimetilmetacrilato e de silicone

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