Effects of pre- and postnatal protein deprivation on rat's hard palatine mucosae: a scanning electron microscopic study of the connective tissue papillae

Boldrini, S.; Watanabe, Ii-sei; Júnior, Bruno König; Liberti, Édson Aparecido

doi:10.1016/s0940-9602(98)80105-2

Cited by 1 publication

(1 citation statement)

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“…Three-dimensional analysis by SEM was performed on two ganglia from each group. Specimens were dissected as previously described and immersed for 24 h at 4ºC in a modified Karnovsky solution containing 2.5% glutaraldehyde and 2.0% paraformaldehyde in PBS (sodium phosphate buffer, 0.1 M, pH 7.4) [14]. After rinsing several times in PBS, specimens were digested by immersion in 5M KOH solution at 60ºC for 5 to 10 min and then for 24 h at 37ºC in a type I collagenase solution (Sigma Aldrich) (1 mg/ml in PBS) [15].…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Impact of Pre and Postnatal Protein Energy Deprivation on Structure of the Trigeminal Ganglion of Weanling Rats

Simões¹,

Boldrini²,

Binotti³

et al. 2009

TONUTRJ

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The effects of protein energy deprivation (PED) on trigeminal ganglion sectional area, neuronal density, number of neurons/section, neuronal body area and neuronal nucleus area were evaluated. The protein deprivation prenatally and for 21 days postnatally leads to reduce body weights (40% of control weights) and an increase in the number of neurons/section (35% more than control). Most neuron perikarya were within 400-600 m 2 (53,7%) in protein-deprived animals and 600-900 m 2 (48,2%) in the control group. Rows of neuronal bodies were separated by bundles of nerve and collagen fibers in both groups. Ganglionic neurons from both groups contained cytoplasmic Nissl's corpuscles and spherical nuclei with eccentric and large nucleoli. The elongated nuclei of the fibrocytes revealed the main axis oriented parallel to the bundles nerve fibers. By scanning electron microscopy, the structure of the ganglion from both groups was found to be similar.

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Section: Scanning Electron Microscopymentioning

confidence: 99%