Effects of pre-freeze incubation of human red blood cells with various sugars on postthaw recovery when using a dextran-rapid cooling protocol

Quan, Guo Bo; Han, Ying; Liu, Min Xia; Gao, Feng

doi:10.1016/j.cryobiol.2009.08.001

Cited by 12 publications

(9 citation statements)

References 37 publications

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“…Higher rates of canine and feline RBC survival after prolonged exposure to ME 2 SO solution may be attributed to the maximum amount of CPA entering erythrocytes, thus implementing cryoprotective action at a higher extent. Similar effect was described at freezing human erythrocytes using dextran and glucose in combination with disaccharides [31]. Authors showed that enhancing of resistance of erythrocytes to cryopreservation was achieved by maximizing the intracellular accumulation of trehalose.…”

Section: Discussionmentioning

confidence: 66%

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Exploring the Possibility of Cryopreservation of Feline and Canine Erythrocytes by Rapid Freezing with Penetrating and Non-Penetrating Cryoprotectants

et al. 2017

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Efficient application of veterinary blood transfusion approaches for small companion animals requires readily available supply of the donor material. This can be achieved by developing of effective biobanking technologies allowing long-term storage of donor blood components via cryopreservation. Transfusion of an erythrocyte concentrate allows the successful correction of various hematological pathologies, severe bleeding, and etc. While in the past there were several approaches to cryopreserve red blood cells of dogs, to our knowledge there is virtually no data on cryopreservation of feline erythrocytes. In this paper, we performed a comprehensive parameter optimization for low temperature storage of RBCs of both species. Here, the efficiency of single-component and multicomponent cryoprotective media as well as necessary time of pre-incubation with penetrating and non-penetrating cryoprotectants prior to rapid freezing is analyzed. This study showed that glycerol was not sufficient for cryopreservation of red blood cells of the studied species under the investigated conditions. Application of 10% (v/v) ME2SO allowed for a significant reduction of canine and feline erythrocytes hemolysis after thawing. 17.5% hydroxyethyl starch demonstrated the highest cryoprotective activity for both species. It was found that dog RBCs should be incubated in cryoprotective media for 30 min at 22°C prior to freezing, while for cat RBCs 20 min is sufficient. Combination of CPAs was less effective. Presented data may be considered in further studies in veterinary transfusion and blood banking optimization.

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Section: Discussionmentioning

confidence: 66%

“…The samples were frozen in 5 mL cryo vials (Eppendorf, Hamburg, Germany) by immersion into liquid nitrogen (rapid freezing protocol). The cooling rate under such conditions is 2.5°C/sec [31]. Thawing of the samples was performed by transferring of container from the liquid nitrogen (-196°C) to a water bath with a constant shaking at 42°C.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Possibility of Cryopreservation of Feline and Canine Erythrocytes by Rapid Freezing with Penetrating and Non-Penetrating Cryoprotectants

et al. 2017

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“…12 Such cryoprotectants are also challenging to remove post thaw; a process which is incompatible with most clinical requirements. 13,14 Analysis of the cryopreservation process, however, shows that extensive cell damage actually arises during the thawing process whereby small ice crystals grow in size in a process known as ice recrystallisation (IR), a form of Ostwald ripening. 15,16 Antifreeze (glyco)proteins (AF(G)Ps) 17 that have arisen by convergent evolution in polar fish species 18 have been shown to be highly potent ice recrystallisation inhibitors (IRIs).…”

Section: Introductionmentioning

confidence: 99%

Ice recrystallisation inhibition by polyols: comparison of molecular and macromolecular inhibitors and role of hydrophobic units

et al. 2013

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“…Under normal conditions, however, trehalose does not easily cross cellular membranes. A number of procedures have been developed to overcome the impermeability of cellular membranes for trehalose 11–14…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of liposomes, trehalose, and hydroxyethyl starch for cryopreservation of human erythrocytes

Stoll

Holovati

Acker

et al. 2012

Biotechnology Progress

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Red blood cells (RBCs) can be cryopreserved using glycerol as a cryoprotective agent, but one of the main disadvantages is the time-consuming deglycerolization step. Novel cryopreservation strategies for RBCs using nontoxic cryoprotective agents are urgently needed. The effect of DMPC, DOPC, and DPPC liposomes on survival of RBCs cryopreserved with trehalose and HES has been evaluated. DMPC caused hemolysis before freezing and affected RBC deformability parameters. DMPC treated RBCs displayed a strong increase in trehalose uptake compared to control cells, whereas DOPC treated liposomes only displayed a slight increase in trehalose uptake. High intracellular trehalose contents were observed after cryopreservation. The recovery of cells incubated with trehalose and liposomes, frozen in HES ranged between 92.6 and 97.4% immediately after freezing. Recovery values of RBCs frozen in HES, however, decreased to 66.5% after 96 h at 4°C compared to 77.5% for DOPC treated RBCs. The recovery of RBCs incubated and frozen in trehalose medium was 77.8%. After 96 hours post-thaw storage recovery of these cells was 81.6%. DOPC and DPPC treated RBCs displayed higher recovery rates (up to 89.7%) after cryopreservation in trehalose compared to control RBCs. Highest survival rates were obtained using a combination of trehalose and HES: 97.8% directly after thawing and 81.8% 96-h post-thaw. DOPC liposomes, trehalose and HES protect RBCs during cryopreservation in a synergistic manner. The advantage is that the protective compounds do not need to be removed before transfusion.

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Effects of pre-freeze incubation of human red blood cells with various sugars on postthaw recovery when using a dextran-rapid cooling protocol

Cited by 12 publications

References 37 publications

Exploring the Possibility of Cryopreservation of Feline and Canine Erythrocytes by Rapid Freezing with Penetrating and Non-Penetrating Cryoprotectants

Exploring the Possibility of Cryopreservation of Feline and Canine Erythrocytes by Rapid Freezing with Penetrating and Non-Penetrating Cryoprotectants

Ice recrystallisation inhibition by polyols: comparison of molecular and macromolecular inhibitors and role of hydrophobic units

Synergistic effects of liposomes, trehalose, and hydroxyethyl starch for cryopreservation of human erythrocytes

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