Effects of pre-reacted glass-ionomer cement on the viability and odontogenic differentiation of human dental pulp cells derived from deciduous teeth

Fujita, Maiko; Mikuni-Takagaki, Yuko; Komori, Reika; Okubo, Koichiro; Yasuda, Mitsuhiro; Kimoto, Shigenari

doi:10.1016/j.pdj.2016.02.003

Cited by 4 publications

(8 citation statements)

References 31 publications

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“…Fujita et al in 2016 studied the effects of pre-reacted glass-ionomer (PRG) cement on the odontogenic differentiation of human dental pulp cells derived from deciduous teeth (hDPC-Ds) using alkaline phosphatase (ALP) activity and immunocytochemistry [ 24 ]. These authors reported that the PRG cement extracts significantly enhanced the ALP activity, ALP staining in the extracellular matrix of hDPC-Ds, and also the release of F and Al ions which enhanced the differentiation of hDPC-Ds.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the high degree of cross linking between silica and GIC makes the nanoHA–silica–GIC much stronger in hardness compared to cGIC [ 21 ]. Studies have been previously carried out to investigate the odontogenic differentiation potential of human dental pulp cells (hDPCs) from deciduous teeth using pre-reacted glass–ionomer cement [ 24 ], hydrogel scaffolds from decellularized bone extracellular matrix and collagen type 1 [ 25 ], dental pulp stem cells (DPSCs) on tricalcium phosphate (TCP) scaffolds [ 26 ], and three bioactive materials, namely, nanoHA, mineral trioxide aggregate, and calcium-enriched mixture cements [ 27 ]. Kwon and colleagues investigated the effects of triethylene glycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA) on the odontogenic differentiation of human dental pulp cells (HDPCs) [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite–Silica–Glass Ionomer Cement

et al. 2020

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This study aimed to investigate the effects of nanohydroxyapatite–silica–glass ionomer cement (nanoHA–silica–GIC) on the differentiation of dental pulp stem cells (DPSCs) into odontogenic lineage. DPSCs were cultured in complete Minimum Essential Medium Eagle—Alpha Modification (α-MEM) with or without nanoHA–silica–GIC extract and conventional glass ionomer cement (cGIC) extract. Odontogenic differentiation of DPSCs was evaluated by real-time reverse transcription polymerase chain reaction (rRT–PCR) for odontogenic markers: dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1), osteocalcin (OCN), osteopontin (OPN), alkaline phosphatase (ALP), collagen type I (COL1A1), and runt-related transcription factor 2 (RUNX2) on day 1, 7, 10, 14, and 21, which were normalized to the house keeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Untreated DPSCs were used as a control throughout the study. The expressions of DSPP and DMP1 were higher on days 7 and 10, that of OCN on day 10, those of OPN and ALP on day 14, and that of RUNX2 on day 1; COL1A1 exhibited a time-dependent increase from day 7 to day 14. Despite the above time-dependent variations, the expressions were comparable at a concentration of 6.25 mg/mL between the nanoHA–silica–GIC and cGIC groups. This offers empirical support that nanoHA–silica–GIC plays a role in the odontogenic differentiation of DPSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite–Silica–Glass Ionomer Cement

et al. 2020

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“…Furthermore, the S‐PRG cements enhance the proliferation and differentiation of human dental pulp cells by a 50% increase in cell number. The increase subsequently induces odontoblastic differentiation thereby aiding pulpal preservation . The future of S‐PRG containing materials appears promising, though further research is required to elucidate the specific effects of the released ions with the quality of the secondary dentine produced.…”

Section: Endodontic Materialsmentioning

confidence: 99%

“…The increase subsequently induces odontoblastic differentiation thereby aiding pulpal preservation. 59 The future of S-PRG containing materials appears promising, though further research is required to elucidate the specific effects of the released ions with the quality of the secondary dentine produced. Furthermore, in vivo research is required to adequately compare S-PRG materials against their counterparts.…”

Section: 2637mentioning

confidence: 99%

Oroactive dental biomaterials and their use in endodontic therapy

et al. 2019

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Dental biomaterials have revolutionized modern therapies. Untreated dental caries remains the major etiological factor for endodontic treatment, and together with a decreasing rate of tooth loss escalates the importance of continuously improving the materials used for endodontic therapies. Endodontic biomaterials are used for vital pulp therapies, irrigation, intracanal medicaments, obturation and regenerative procedures. These materials offer several functions including: antimicrobial activity, mechanical reinforcement, aesthetics, and therapeutic effects. Vital pulp therapies have seen an improvement in clinical results with an incremental approach to build on the strengths of past materials such as calcium hydroxide and calcium silicates. While sodium hypochlorite remains the gold standard for canal irrigation, numerous nanoparticle formulations have been developed to promote sustained antimicrobial action. Gutta‐percha based bulk fillers remain the most common materials for root filling. However, while multiple studies focus on the development of novel formulations containing drugs, glass derivatives or ionic‐, polymeric‐, or drug‐ loaded nanoparticles, a lack of reliable and long‐term clinical evidence obligates further study as experienced clinicians prefer to use what has worked for decades. This review delves in to the biochemistry of the materials to scrutinize their shortcomings, and where opportunity lies to further enhance their efficacy in endodontic practice. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:201–212, 2020.

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“…Meanwhile, in another study, S-PRG cement had similar characteristics to mineral trioxide aggregate concerning the quality and quantity of their dentin bridge forming ability and it showed low cytotoxicity and favourable remineralization-induction properties [17]. Fujita et al [18] studied the effect of cement that contained S-PRG filler on human dental pulp cells derived from deciduous teeth, in which the proliferation and differentiation of the cells were enhanced. The abovementioned behaviours of S-PRG filler were mostly attributed to the release of multiple ions, and more attention on this aspect may be used to enhance the effect of S-PRG filler with the addition of other ions, such as lithium [19].…”

Section: Introductionmentioning

confidence: 99%

The effect of surface pre‐reacted glass‐ionomer filler eluate on dental pulp cells and mineral deposition on dentin: In vitro study

Nishimaki

Nassar

Tamura

et al. 2021

European J Oral Sciences

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The effects of surface pre-reacted glass-ionomer (S-PRG) filler on pulpal cells and on the composition of dentinal deposits were investigated. Proliferation (CCK-8), cytotoxicity (LDH), and differentiation activity (ALP) tests, along with cell morphology observations, were conducted at 6 and 24 h after treatment of pulpal cells with different S-PRG filler eluate concentrations. Dentinal surfaces were immersed in deionized water or S-PRG filler eluate followed by immersion in deionized water or simulated body fluid and observed under scanning electron microscope and elemental analysis using energy dispersive x-ray spectrometer. At 24 h, there were significant differences in CCK-8 and ALP activity values between the groups in a concentrationdependent manner. LDH test data were not significantly different among the groups.Cell morphology was not altered at either exposure time. However, decreased cellular density was observed with the highest eluate concentration. Crystalline deposits and occluded dentinal tubules were observed in samples immersed in S-PRG filler with a later immersion in simulated body fluid, which also showed higher concentrations of certain ions compared to surfaces that were not initially treated with S-PRG filler. The lowest two eluate concentrations did not show significant toxicity. S-PRG enhanced the effect of simulated body fluid in the formation of mineral deposits.

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Effects of pre-reacted glass-ionomer cement on the viability and odontogenic differentiation of human dental pulp cells derived from deciduous teeth

Cited by 4 publications

References 31 publications

Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite–Silica–Glass Ionomer Cement

Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite–Silica–Glass Ionomer Cement

Oroactive dental biomaterials and their use in endodontic therapy

The effect of surface pre‐reacted glass‐ionomer filler eluate on dental pulp cells and mineral deposition on dentin: In vitro study

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