Effects of pre-storage hydration treatments on germination performance, moisture content, DNA synthesis and controlled deterioration tolerance of tomato (<i>Lycopersicon esculentum</i> Mill.) seeds

Pijlen, J.G. van; Groot, Steven P. C.; Kraak, H.L.; Bergervoet, J.H.W.; Bino, R.J.

doi:10.1017/s0960258500003032

Cited by 38 publications

(24 citation statements)

References 20 publications

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“…This technique induces faster and more uniform seed germination over a broad temperature range (McDonald, 1999) and under drought conditions in semiarid countries (Murungu et al, 2004; Harris et al, 1999). This improvement in seed germination has been shown in maize ( Zea mays L.) (Afzal et al, 2002; Dezfuli et al, 2008), tomato ( Lycopersicon lycopersicum L.) (Van Pijlen et al, 1996), and sunflower ( Helianthus annuus L.) (Hussain et al, 2006). Enhanced maize seed vigor as affected by hydropriming has been reported by Wattanakulpakin et al (2010).…”

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confidence: 67%

Loss of Maize Seed Vigor as Affected by Biochemical Changes during Hydropriming

2012

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Hydropriming has both positive and negative effects on seed performance. Hydropriming (for 6 to 30 h) was performed in the maize {Zea mays L.) seed iines TSK 11' and 'PS 54'. A short hydropriming time of 6 to 12 h improved seed germination in both lines. An improvement in seed vigor, as investigated by germination after accelerated aging, was observed after hydropriming for 6 and 12 h in TSK 11' seeds, but only after 6 h of hydropriming in 'PS 54'. The reduction in seed vigor occurred faster in 'PS 54' than in 'TSK 11', which was confirmed at hydropriming times of 12 and 18 h, respectively. The higher activities of antioxidant enzymes, that is, Superoxide dismufase (SOD) and ascorbate peroxidase (APX) in 'TSK 11', and SOD, peroxidase (POD), and APX in 'PS 54', correlated with enhanced maize seed vigor after priming. The deterioration of primed maize seeds was linked to the process of lipid peroxidation, indicated by the generation of malondialdehyde during imbibition, and acetaldehyde and ethanol during redrying. Additional indicators were identified: a reduction in the antioxidant activities of SOD, GAT, and APX for 'TSK 11 ', and SOD and CAT for 'PS 54', decreased raffinose content, and increased amylase activity. A less compact cellular ultrastructure was observed in hydroprimed seeds and was associated with seed deterioration. The different responses in physiological and biochemical attributes induced by hydropriming were related fo their genotype and partially affected by fhe initial characferisfic differences befween fhe two maize lines causing different maize seed appearance.

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confidence: 67%

Loss of Maize Seed Vigor as Affected by Biochemical Changes during Hydropriming

2012

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“…This implies that during osmoconditioning the cell cycle was arrested, allowing the synchronization of cells in G 2 . Apparently, this is a checkpoint controlled by the osmoticum and explains why the number of 4C or G 2 nuclei becomes invariable after 7 d of osmoconditioning (van Pijlen et al, 1996). Furthermore, mitotic events and cell divisions occurred earlier in embryos of primed seeds upon subsequent imbibition in water and in higher numbers than in the control seeds.…”

Section: Enhanced Germination Performance Of Osmoconditioned Seeds Ismentioning

confidence: 96%

Cell Division and Subsequent Radicle Protrusion in Tomato Seeds Are Inhibited by Osmotic Stress But DNA Synthesis and Formation of Microtubular Cytoskeleton Are Not

Castro

Lammeren

Groot³

et al. 2000

Plant Physiology

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We studied cell cycle events in embryos of tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds during imbibition in water and during osmoconditioning ("priming") using both quantitative and cytological analysis of DNA synthesis and ␤-tubulin accumulation. Most embryonic nuclei of dry, untreated control seeds were arrested in the G 1 phase of the cell cycle. This indicated the absence of DNA synthesis (the S-phase), as confirmed by the absence of bromodeoxyuridine incorporation. In addition, ␤-tubulin was not detected on western blots and microtubules were not present. During imbibition in water, DNA synthesis was activated in the radicle tip and then spread toward the cotyledons, resulting in an increase in the number of nuclei in G 2 . Concomitantly, ␤-tubulin accumulated and was assembled into microtubular cytoskeleton networks. Both of these cell cycle events preceded cell expansion and division and subsequent growth of the radicle through the seed coat. The activation of DNA synthesis and the formation of microtubular cytoskeleton networks were also observed throughout the embryo when seeds were osmoconditioned. However, this preactivation of the cell cycle appeared to become arrested in the G 2 phase since no mitosis was observed. The pre-activation of cell cycle events in osmoconditioned seeds appeared to be correlated with enhanced germination performance during re-imbibition in water.

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“…These invigoration techniques include seed soaking/seed priming/pelleting treatments. Spraying of a water mist over the seeds is considered a simple hydro priming technique which allows the moisture to equilibrate (Van Pijlen et al, 1996) and enhance early germination. Various organic seed treatment preparations (biodynamic and herbal) have been recommended to be applied as spray on seeds followed by quick drying to encourage germination and seedling establishment (Courtney, 1994).…”

Section: Discussionmentioning

confidence: 99%