Effects of pregnancy-specific β-1-glycoprotein on the helper T-cell response

Тимганова, В. П.; Бочкова, М. С.; Khramtsov, Pavel; Kochurova, Sofia; Rayev, M. B.; Заморина, С. А.

doi:10.2298/abs190122019t

Cited by 9 publications

(5 citation statements)

References 65 publications

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“…Our previous study showed the general inhibitory effect of PSG on the proliferation of helper T cells (CD4 + ) under conditions of Th17-polarization [13]. In addition, we also found that PSG inhibits the proliferation of T helpers in the presence of IL-2 while preventing the conversion of naïve T cells into a terminally differentiated effector T -helpers [12]. Considering that PSG affected cells via activation of the latent form of TGF-β1 [4], we suggest that TGF-β1-mediated antiproliferative effects of PSG are versatile for all lymphocytes.…”

Section: Resultsmentioning

confidence: 59%

Effect of Pregnancy Specific β1-Glycoprotein on the Replicative Potential of Naïve T Cells and Immune Memory T Cells

et al. 2021

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We studied the effects of pregnancy-specific β1-glycoprotein (PSG) on the replicative potential of naïve T cells (CD45RA + ) and immune memory T cells (CD45R0 + ) in vitro by evaluating the expression of the hTERT gene in combination with the proliferative activity of cells. Human PSG was obtained by the author's patented method of immunopurification using a biospecific sorbent with subsequent removal of immunoglobulin contamination on a HiTrap Protein G HP column. We used monocultures of CD45RA + and CD45R0 + lymphocytes isolated from peripheral blood mononuclear cells of reproductive-age women. It was found that PSG in physiological concentrations inhibited the expression of the hTERT gene mRNA in naïve T cells and immune memory T cells and simultaneously reduced the number of proliferating T cells estimated by the differential gating method. At the same time, PSG reduced CD71 expression only on naïve T cells without affecting this molecule on immune memory T cells. Thus, PSG decreased the replication potential and suppressed the proliferation of T cells and immune memory T cells, which in the context of pregnancy can contribute to the formation of immune tolerance to the semi-allogeneic embryo.

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Section: Resultsmentioning

confidence: 59%

Effect of Pregnancy Specific β1-Glycoprotein on the Replicative Potential of Naïve T Cells and Immune Memory T Cells

et al. 2021

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“…The cells form fairly distinct populations that can be gated, and you can calculate what percentage of the total number of cells ends up in each gate. This method was previously described in our article [71], and in a subsequent article [72], we showed that the percentage of proliferating cells obtained by differential gating correlated with the percentage of Ki67 + cells.…”

Section: Flow Cytometrymentioning

confidence: 70%

Effects of glycodelin on CCR6<sup>+</sup> cell subpopulations of Th17-polarized helper T cells

Timganova,

Zamorina,

Bochkova

et al. 2023

Med. immunol.

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Glycodelins, the glycosylated proteins of reproductive tract are characterized by immunomodulatory functions, are of interest because of their role in the development of immune tolerance. Interleukin-17-producing T helpers (Th17) bearing the surface marker CCR6, are a heterogeneous cell population with increased plasticity and functional dichotomy. On the one hand, these cells support antimicrobial and antifungal immunity and microbiota composition; on the other hand, they are involved in the pathogenesis of autoimmune diseases, graft rejection, and pregnancy complications. Despite the scientific interest in glycodelin as an immunomodulator, its direct effects on pro-inflammatory Th17 have not been studied. Therefore, the aim of our work was to investigate the effect of recombinant human glycodelin on Th17 polarization of naïve human T helper cells cells by assessing surface expression of CCR6, CCR4, and CXCR3 molecules. Naïve T helper cells were polarized for 7 days in vitro to Th17 cells with a TCR activator and cytokines for 7 days, supplemented with glycodelin at concentrations appropriate for the 1st and 2nd trimesters of pregnancy. The percentages of CD4+CCR6+ cell population (Th17 cells), and their CCR4+CXCR3-(Th17/Th22) and CCR4-CXC3+ subpopulations (Th17.1) was then determined. Moreover, the levels of IL-17, IL-2, and other cytokines/chemokines were determined in the culture supernatants of Th17-polarized T helper cells. Treatment with recombinant glycodelin at concentrations equivalent to those in pregnancy (0.2, 2, and 10 μg/mL) did not alter the percentage of CD4+CCR6+ cells in culture, or their IL-17 production. However, at a concentration of 10 μg/mL, it caused a decrease in Th17.1 (CCR6+CCR4-CXCR3+) percentage in the T helper culture, and increased the production of IL-2. In addition, glycodelin was found to have selective pro-apoptotic activity against Th17.1 if applied at 2 μg/mL. Given the known involvement of these cells in pathological processes, the observed effect of glycodelin could be of interest from a biopharmaceutical perspective. However, the mechanism of the revealed selective effects of this pregnancy protein needs further investigation.

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“…On the FSC/SSC plot, cells were first gated (cell debris was excluded), and then gates of non-proliferating, proliferating, and apoptotic cells were selected in the cell gate ( Figure 4 ). Then, the percentage of cells falling into each of these gates was determined from the total number of cells [ 26 , 27 ]. Data were collected using a CytoFLEX S flow cytometer and analyzed using CytExpert software (Beckman Coulter, Brea, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

The Effect of PEGylated Graphene Oxide Nanoparticles on the Th17-Polarization of Activated T Helpers

et al. 2023

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We investigated the direct effect of PEGylated graphene oxide (P-GO) nanoparticles on the differentiation, viability, and cytokine profile of activated T helper type 17 (Th17) in vitro. The subject of the study were cultures of “naive” T-helpers (CD4+) isolated by immunomagnetic separation and polarized into the Th17 phenotype with a TCR activator and cytokines. It was found that P-GO at low concentrations (5 µg/mL) had no effect on the parameters studied. The presence of high concentrations of P-GO in T-helper cultures (25 μg/mL) did not affect the number and viability of these cells. However, the percentage of proliferating T-helpers in these cultures was reduced. GO nanoparticles modified with linear polyethylene glycol (PEG) significantly increased the percentage of Th17/22 cells in cultures of Th17-polarized T helpers and the production of IFN-γ, whereas those modified with branched PEG suppressed the synthesis of IL-17. Thus, a low concentration of PEGylated GO nanoparticles (5 μg/mL), in contrast to a concentration of 25 μg/mL, has no effect on the Th17-polarization of T helpers, allowing their further use for in-depth studies of the functions of T lymphocytes and other immune cells. Overall, we have studied for the first time the direct effect of P-GO nanoparticles on the conversion of T helper cells to the Th17 phenotype.

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Effects of pregnancy-specific β-1-glycoprotein on the helper T-cell response

Cited by 9 publications

References 65 publications

Effect of Pregnancy Specific β1-Glycoprotein on the Replicative Potential of Naïve T Cells and Immune Memory T Cells

Effect of Pregnancy Specific β1-Glycoprotein on the Replicative Potential of Naïve T Cells and Immune Memory T Cells

Effects of glycodelin on CCR6<sup>+</sup> cell subpopulations of Th17-polarized helper T cells

The Effect of PEGylated Graphene Oxide Nanoparticles on the Th17-Polarization of Activated T Helpers

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