Effects of Preincubating Blood Culture Bottles at 37℃ during the Night Shift and of Collected Blood Volume on Time to Detection and Days to Final Report

Koh, Eun-Ha; Lee, Dong-Hyun; Kim, Sun Joo

doi:10.5145/acm.2014.17.1.14

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…Traditionally, in many settings, during nightshifts and weekends, pre-incubation at 37°C without growth monitoring is available as well. Both Koh et al [34] and van der Velden et al [35] demonstrated that this procedure enabled earlier final reports than storage at room temperature. However, as reported by several authors, pre-incubation at 37°C increases false-negative rate, especially if it lasts for long time [22, 23, 27–30, 32, 35].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have identified several logistic factors able to act as significant predictors of longer storage times, including clinical ward or laboratory off-site location, clinical speciality, time of sampling and number of transports per day [12, 14]. Both the clinical and financial benefits related to a more rapid bacterial identification have been already reported by several authors [1, 2, 5, 13, 34–35, 38]. The financial costs of implementing potentially effective interventions can be significantly different depending on the action chosen.…”

Section: Discussionmentioning

confidence: 99%

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Impact of Pre-Analytical Time on the Recovery of Pathogens from Blood Cultures: Results from a Large Retrospective Survey

et al. 2017

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BackgroundPrompt identification of bloodstream pathogens is essential for optimal management of patients. Significant changes in analytical methods have improved the turnaround time for laboratory diagnosis. Less attention has been paid to the time elapsing from blood collection to incubation and to its potential effect on recovery of pathogens. We evaluated the performance of blood cultures collected under typical hospital conditions in relation to the length of their pre-analytical time.MethodsWe carried out a large retrospective study including 50,955 blood cultures collected, over a 30-month period, from 7,035 adult septic patients. Cultures were accepted by the laboratory only during opening time (Mon-Fri: 8am–4pm; Sat: 8am–2pm). Samples collected outside laboratory hours were stored at room temperature at clinical wards. All cultures were processed by automated culture systems. Day and time of blood collection and of culture incubation were known for all samples.ResultsA maximum pre-analytical interval of 2 hours is recommended by guidelines. When the laboratory was open, 57% of cultures were processed within 2 h. When the laboratory was closed, 4.9% of cultures were processed within 2 h (P<0.001). Samples collected when the laboratory was closed showed pre-analytical times significantly longer than those collected when laboratory was open (median time: 13 h and 1 h, respectively, P<0.001). The prevalence of positive cultures was significantly lower for samples collected when the laboratory was closed compared to open (11% vs 13%, P<0.001). The probability of a positive result decreased of 16% when the laboratory was closed (OR:0.84; 95%CI:0.80–0.89, P<0.001). Further, each hour elapsed from blood collection to incubation resulted associated with a decrease of 0.3% (OR:0.997; 95%CI:0.994–0.999, P<0.001) in the probability of a positive result.DiscussionDelayed insertions of cultures into automated systems was associated with lower detection rates, with potentially important consequences for patients. In each hospital setting the logistic factors able to shorten pre-analytical time should be carefully investigated and specifically targeted.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Impact of Pre-Analytical Time on the Recovery of Pathogens from Blood Cultures: Results from a Large Retrospective Survey

et al. 2017

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“…18 Our study results are in concordance with the study by Eon-Ha Kohl et al done in 2013, which showed that 37℃ pre-incubation was advantageous over RT in detection of organisms in blood culture systems, when delayed entry is inevitable. 19 BACTEC bottles detected Pseudomonas aeruginosa, Streptococcus and candida albicans earlier whereas for E. coli and S. aureus were detected faster by BACTALERT, whereas a study done by Cockerell et al showed early detection of Enterobacteriaceae, Pseudomonas spp, S.aureus and Candida species by BACTEC. 20 BACTEC showed the lowest average TTD at the holding time of 12hrs at 4°C, 24hrs at RT and 12hrs at 37°C respectively.…”

Section: Discussionmentioning

confidence: 89%

Effect of delayed vial entry on the recovery and time to positivity of microorganisms from automated blood culture vials in a tertiary care hospital, South India

Samantaray¹,

Abisha²,

Prakash³

et al. 2020

IJMMTD

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Recommendations by manufacturers depict that blood culture bottles are to be loaded into the automated machines as soon as possible after the blood collection. But it has been noticed that in peripheral set-ups where round the clock laboratory facility is not available or which are located far from the reference labs, or even in the tertiary care centres, delay occurs prior to the bottle loading into the instruments. In our study, we have compared the effect of pre-incubating the blood culture bottles at various temperatures like 4°C, room temperature and 37°C for various holding times, such as 2, 6, 12 and 24 hours simulating the delay in transport time in BACTEC plus Aerobic and BACTALERT FA plus systems in a tertiary care set up. We included five recent clinical isolates of different microorganisms in our study, such as Staphylococcus aurous, Streptococcus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Standard inoculum was prepared for each organism and 3 ml of it was added with 10 ml of citrated human blood and then inoculated into blood culture vial (BACTALERT FA plus and BACTEC Plus). Organism recovery rate, time to detection and rates of false-negativity from both the instruments were evaluated by using seeded blood culture vial and controls with delayed entry. Performance wise analysis showed that the overall isolation rate of the organisms from BACTEC bottles was higher compared to BACTALERT. Also BACTEC showed less average TTD compared to BACTALERT for Pseudomonas aeruginosa, Streptococcus and candida albicans but for E. coli and S. aureus BACTALERT showed earlier detection. To conclude, storage of the inoculated bottles at room temperature gave optimal recovery of the organisms for both BACTEC and BACTALERT systems, if delayed entry is inevitable.

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“…Koh et al (5) indicated that the blood culture bottles that were pre-incubated at 37°C during the night ensure earlier final reports. Also, Lee et al (6) reported that TTD was influenced by the pre-incubation temperature and duration rather than the colony-forming unit quality or bottle type.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Rapid Identification of Metisillin Resistance of Staphylococcus Aureus (MRSA) in Blood Culture Bottles at Three Different Pre-Incubation Periods

Güvenir¹,

Güler²,

Aykac³

et al. 2018

Cyprus J Med Sci

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Effects of Preincubating Blood Culture Bottles at 37℃ during the Night Shift and of Collected Blood Volume on Time to Detection and Days to Final Report

Cited by 7 publications

References 9 publications

Impact of Pre-Analytical Time on the Recovery of Pathogens from Blood Cultures: Results from a Large Retrospective Survey

Impact of Pre-Analytical Time on the Recovery of Pathogens from Blood Cultures: Results from a Large Retrospective Survey

Effect of delayed vial entry on the recovery and time to positivity of microorganisms from automated blood culture vials in a tertiary care hospital, South India

Investigation of Rapid Identification of Metisillin Resistance of Staphylococcus Aureus (MRSA) in Blood Culture Bottles at Three Different Pre-Incubation Periods

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