Effects of prenatal protein malnutrition on hippocampal CA1 pyramidal cells in rats of four age groups

Cintra, L.; Aguilar, Azucena; Granados, Leticia; Galván, A.; Kemper, Thomas L.; DeBassio, William; Galler, Janina R.; Morgane, Peter J.; Durán, Pilar; Dı́az-Cintra, Sofı́a

doi:10.1002/(sici)1098-1063(1997)7:2<192::aid-hipo6>3.0.co;2-p

Cited by 57 publications

(18 citation statements)

References 45 publications

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“…A study on CA1 hippocampal pyramidal cells was the third in a series of morphometric studies on the effect of prenatal protein restriction on key neurones in the hippocampal trisynaptic circuit (Cintra et al 1997a). When compared with their previous studies on the dentate granule cell and the CA3 pyramidal cell, it was found that a low-protein diet affects all of these neurones, with the most marked effect being on the predominantly postnatally generated dentate granule cells.…”

Section: Prenatal Protein Malnutrition Hippocampal Development and Bmentioning

confidence: 99%

Nutrition and neurodevelopment: mechanisms of developmental dysfunction and disease in later life

Dauncey

Bicknell

1999

Nutr. Res. Rev.

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Nutrition plays a central role in linking the ®elds of developmental neurobiology and cognitive neuroscience. It has a profound impact on the development of brain structure and function and malnutrition can result in developmental dysfunction and disease in later life. A number of diseases, including schizophrenia, may be related to neurodevelopmental insults such as malnutrition, hypoxia, viruses or in utero drug exposure. Some of the most signi®cant ®ndings on nutrition and neurodevelopment during the last three decades, and especially during the last few years, are discussed in this review. Attention is focused on the underlying cellular and molecular mechanisms by which diet exerts its effects. Randomized intervention studies have revealed important effects of early nutrition on later cognitive development, and recent epidemiological ®ndings show that both genetics and environment are risk factors for schizophrenia. Particularly important is the effect of early nutrition on development of the hippocampus, a brain structure important in establishing learning and memory, and hence for cognitive performance. A major aim of future research should be to elucidate the molecular mechanisms underlying nutritionally-induced impairment of neurodevelopment and speci®cally to determine the mechanisms by which early nutritional experience affects later cognitive performance. Key research objectives should include: (1) increased understanding of mechanisms underlying the normal processes of ageing and neurodegenerative disorders; (2) assessment of the role of susceptibility genes in modulating the effects of early nutrition on neurodevelopment; and (3) development of nutritional and pharmaceutical strategies for preventing and/or ameliorating the adverse effects of early malnutrition on long-term programming.

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Section: Prenatal Protein Malnutrition Hippocampal Development and Bmentioning

confidence: 99%

Nutrition and neurodevelopment: mechanisms of developmental dysfunction and disease in later life

Dauncey

Bicknell

1999

Nutr. Res. Rev.

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“…In our earliest studies of the effects of prenatal malnutrition on hippocampal anatomy, we found differential changes of pyramidal cell morphology in both the CA3 (García-Ruíz et al, 1993;Díaz-Cintra et al, 1994) and CA1 (Cintra et al, 1997a) areas and in the granular cells (second limb that projects to the stratum lucidum of the CA3 pyramidal cells) (Cintra et al, 1990;Díaz-Cintra et al, 1991). Among these, we reported that the most marked effect of the restricted diet is on the granular cells.…”

Section: Introductionmentioning

confidence: 99%

The Mossy Fiber System of the Hippocampal Formation is Decreased by Chronic and Postnatal but not by Prenatal Protein Malnutrition in Rats

Granados-Rojas

Aguilar

Dı́az-Cintra

2004

Nutritional Neuroscience

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We tested in 70-day-old Sprague-Dawley rats, whether malnutrition imposed during different periods of hippocampal development produced deleterious effects on the total reference volume of the mossy fiber system. Animals were treated under four nutritional conditions: (a) well nourished; (b) prenatal protein malnourished; (c) chronic protein malnourished and (d) postnatal protein malnourished. Timm's stained material was used in coronal hippocampal sections (40 microm) to estimate--using the Principle of Cavalieri--the total reference volume of the mossy fiber system in each experimental group. Our results show that chronic and postnatal protein malnourished, but not prenatal malnourished rats, decrease the mossy fiber system and the total reference volume of the mossy fiber system are selectively vulnerable to the type of dietary restriction. Thus, chronic and posnatal protein malnutrition produce deleterious effects, but only rats under prenatal protein malnutrition were able to reorganize synapses in this plexus. These findings raise the possibility that chronic malnutrition, as a long-term stressful factor, might be an important paradigm to test structural hippocampal changes that produce physiological and pathophysiological effects, or the possibility to recover its function for nutritional rehabilitation.

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“…Experiments using animal models have shown that severe food restriction (undernutrition or malnutrition) during brain development permanently inhibits brain growth in size (Garcia-Ruiz et al 1993, Cintra et al 1997) and affects axonal growth and myelination of optic nerves (Sima and Sourander 1974, 1978, Wiggins et al 1984, Wender and Goncerzewicz 1986, Royland et al 1993, Vargas et al 2000. Winnings et al (1984) found a significant myelin reduction in the optic nerve when rats were deprived from the dam for a number of hours that progressively increased everyday up to 12 h per day in the second week.…”

Section: Discussionmentioning

confidence: 82%

Quantitative ultrastructural evidence of myelin malformation in optic nerves of rats submitted to a multideficient diet

Almeida

Silveira

Guedes

et al. 2005

Nutritional Neuroscience

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Pups were subjected to malnutrition by feeding the lactating mothers a multi-deficient (8% protein content) diet, known as regional basic diet (RBD), from birth up to weaning. The weanings were fed the same diet until 60 days of age. Ultrastructure of the optic nerve was analyzed at postnatal (P) day P8, P13, P21, P30 and P60. Electron microscopy revealed that at P8 the process of myelination has not started yet in neither groups. At P 13 different stages of myelination were observed and, in the experimental group, the optic nerve showed non-organized axon bundles and empty spaces. Control optic nerve at P21 exhibited axons with fully developed myelin sheath; whereas malnourished group had many axons being enveloped by myelin with anomalous alteration. These alterations were present in malnourished group at P30 and P60. Quantitative analysis showed statistically significant difference between control and malnourished groups when compared to the percentage of myelinated axons, axons with myelin anomalous alterations (MAA) and non-myelinated axons. Also, the myelin area was significantly smaller in malnourished groups when compared to control group. Finally, a high percentage of large non-myelinated fibers were found in the malnourished group. In conclusion, our results show that early malnutrition by a multideficient diet (RBD) affects permanently the optic nerve organization and myelination, indicating an impairment of nerve transmission and a probable dysfunction in the visual ability.

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Effects of prenatal protein malnutrition on hippocampal CA1 pyramidal cells in rats of four age groups

Cited by 57 publications

References 45 publications

Nutrition and neurodevelopment: mechanisms of developmental dysfunction and disease in later life

Nutrition and neurodevelopment: mechanisms of developmental dysfunction and disease in later life

The Mossy Fiber System of the Hippocampal Formation is Decreased by Chronic and Postnatal but not by Prenatal Protein Malnutrition in Rats

Quantitative ultrastructural evidence of myelin malformation in optic nerves of rats submitted to a multideficient diet

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