Effects of prenatally exposed diclofenac sodium on rat heart tissue:a stereological and histological study

Abstract

Background/aim: Diclofenac sodium (DS) can cross the placental barrier and affect the fetus, and its consumption during pregnancy may cause developmental malformation of embryos. This study investigates the effect of prenatally applied DS on the quantitative morphology of the adult rat heart.Materials and methods: Pregnant rats were divided into three groups (control, sham, and test). The rats in the test group were injected with DS; the control group received physiological saline (1 mL; 1 mg/kg, i.m.) from the 5th to the 20th day of pregnancy; and the rats in the sham group were not injected at all. At the 20th postnatal week, all the offspring were euthanized under deep anesthesia and tissue samples were obtained by perfusion fixation. After routine histological procedures, the paraffin sections were stained with hematoxylin and eosin and examined stereologically and histologically. Results:The volume of the cardiac ventricle wall of each offspring rat was estimated using Cavalieri's principle. The volume of the ventricle walls of the test group was found to be significantly less than that of the controls. Conclusion:Further studies are required to determine how DS has this effect, by reducing the number of myocytes and decreasing the size of these cells affecting the connective tissue.

The effect of diclofenac sodium on neural tube development in the early stage of chick embryos

“…The fact that DS cross the placental barrier to prevent the biosynthesis of prostanoids and passes into the foetal circulation causes important side effects and sometimes malformations in newborns [12,29,45]. Some assays determined that the DS using throughout the perinatal period may cause teratogenic effects on some organs [3,6,16,19]. In addition; the use of DS during embryonic development of the central nervous system (CNS) can produce a wide array of neurological dysfunctions and neuroanatomical anomalies in animal models [9,22].…”

Evaluation of the effects of alcohol on the heart by stereological methods

“…The coefficient of error (CE) was calculated. The error coefficient of our stereological study was acceptable (CE ≤ 0.05) (12,13). The density of grid points and efficiency of sampling were performed as documented in the literature (14).…”

Ingestão De Dicromato De Potássio Induz Alterações Morfológicas Em Cardiomiócitos De Ratos

“…Alterações no tecido cardiovascular estão ligadas à estresse oxidativo e menor expressão de agentes anti-inflamatórios, podendo reduzir o trofismo muscular cardíaco na parede ventricular de ratos expostos à agentes tóxicos 16 . Um estudo 17 verificou que semelhantes alterações teciduais no tecido muscular cardíaco pode ser desencadeado pelo efeito inflamatório modulado pela alta concentração de NF-κB.…”

“…A exposição de células cardíacas a outras substâncias medicamentosas ou tóxicas podem estimular a redução do volume celular do cardiomiócito de ratos, podendo refletir na redução do volume da parede ventricular 20 .…”