Effects of prepartal oronasal administration of lipopolysaccharide on milk composition and productivity of transition Holstein dairy cows

Abstract

The aim of this study was to evaluate the effects of repeated oronasal administration of lipopolysaccharide (LPS) on milk composition and the overall productive performance of dairy cows. One hundred pregnant Holstein dairy cows were randomly assigned to two treatment groups (n = 50). 30 cows out of 100 were selected for intensive sampling (n = 15) starting at 28 d before parturition. Cows were administered orally and nasally with 2 and 1 mL of saline solution, respectively (control), or saline solution containing 3 doses of LPS from Escherichia coli 0111:B4 as follows: 1) 0.01 µg/kg body weight (BW) on d −28, 2) 0.05 µg/kg BW on d −25 and −21, and 3) 0.1 µg/kg BW on d −18 and −14. Daily feed intake and milk production were recorded for each cow during the first 28 d postpartum. Milk samples were obtained once per week and analyzed for various milk components. Overall, results indicated that treatment did not affect feed intake, milk yield, milk efficiency, fat content, fat yield, protein content, protein yield, lactose content, lactose yield, milk urea nitrogen (MUN), total solid contents, fat-corrected milk (FCM), and energy-corrected milk (ECM; P > 0.05). However, milk somatic cell count (SCC) tended to be lower in the treated cows (P < 0.10). Treated primiparous cows showed tendencies for better milk efficiency (P < 0.10), milk-fat content (P = 0.09), and total solid contents (P = 0.06). There was a treatment by week interaction for milk energy (P = 0.03), and tendencies for FCM, ECM, lactose content, and milk efficiency (P < 0.10) with greater values in the treated primiparous animals. Altogether, results of this study showed that oronasal LPS challenges slightly modulated milk composition of periparturient dairy cows.

Effects of continuous low dose infusion of lipopolysaccharide on inflammatory responses, milk production and milk quality in dairy cows

“…Shuster, Harmon, Jackson, and Hemken () also found a decline in milk fat content and lactational performance following intra‐mammary infusion with LPS. The milk protein content after challenge with LPS in our study was similar to the study by Iqbal, Zebeli, Mansmann, Dunn, and Ametaj (), which showed that protein content displayed a downtrend by oronasal LPS administration. Hinz, Larsen, Wellnitz, Bruckmaier, and Kelly () also demonstrated that exposure to LPS profoundly decreased milk protein content following infusion with LPS into the mammary gland.…”