2005
DOI: 10.4319/lom.2005.3.143
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Effects of preservation and storage of microcrustaceans in RNAlateron RNA and DNA degradation

Abstract: Methods of preserving nucleic acids are increasingly in demand because of the recent advances in molecular and biochemical approaches to ecology. The RNA storage reagent RNAlater ® was tested as an alternative method to deep-freezing for preserving RNA and DNA in zooplankton. Artemia spp. nauplii were used as test organisms. Individual RNA and DNA contents were monitored over a time period of 8 months to evaluate the effects of preservation and storage. Treatments included (1) freezing at -80°C, (2) preservati… Show more

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Cited by 92 publications
(77 citation statements)
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“…A closed cooling chain is not mandatory. For preservation of microcrustaceans of zooplankton like copepods, up to a month of storage time is possible without any losses of RNA quality if RNAlater is used (Gorokhova, 2005). Own experiences corroborate this study with samples show a dissected house gecko (Hemidactylus frenatus), which was parasitized by tongue worms (Pentastomida, small picture) in his lunge tract.…”
supporting
confidence: 73%
“…A closed cooling chain is not mandatory. For preservation of microcrustaceans of zooplankton like copepods, up to a month of storage time is possible without any losses of RNA quality if RNAlater is used (Gorokhova, 2005). Own experiences corroborate this study with samples show a dissected house gecko (Hemidactylus frenatus), which was parasitized by tongue worms (Pentastomida, small picture) in his lunge tract.…”
supporting
confidence: 73%
“…In all incubations, survivorship was never below 90%, with no apparent differences between the treatments, including controls (Kruskal-Wallis statistic = 0.89, p > 0.9). Live copepods were individually picked with forceps, rinsed twice in FSW, transferred to Eppendorf tubes containing 100 µl of RNAlater and stored at -20°C for approximately 4 mo (Gorokhova 2005). These animals were used for quantitative PCR (qPCR) analysis.…”
Section: Methodsmentioning
confidence: 99%
“…Zooplankton were collected by vertical tows from 5 m above the bottom to the surface using a 90 µm WP-2 net (diameter 57 cm) equipped with a flow meter. From each tow, randomly selected copepods were preserved in bulk using RNAlater and stored for 1 to 5 wk at 4-5°C until the nucleic acid analysis (Gorokhova 2005). The rest of the single-tow sample was preserved in 4% boraxbuffered formaldehyde for microscopic analysis.…”
Section: Methodsmentioning
confidence: 99%