Codoñer Franch, P.; Betoret Valls, ME.; López Jaén, AB.; Betoret Valls, N.; Fito Maupoey, P.; Valls Bellés, MV. (2013). Dried apple enriched with mandarin juice counteracts tamoxifen induced oxidative stress in rats. International Journal of Food Sciences and Nutrition. 64(7): 815-821. doi:10.3109/09637486.2013.798267. Dried apple enriched with mandarin juice by vacuum impregnation counteracts the liver oxidative effect of tamoxifen in rats.
AbstractWe have investigated the effects of a product made of apple dehydrated enriched by vacuum impregnation of mandarin juice in decreasing oxidative stress biomarkers in rat induced liver injury by tamoxifen. A experimental design was undertaken in six groups of animals. One group fed a standard diet. Two groups were supplemented with 0.745 g/d of apples snacks/day either impregnated or not with mandarin juice during 28 days. Three groups of rats were treated with tamoxifen 1.54 mg/kg/day during 21 days; of these, one group was supplemented with the apple snacks and the other with the apple snacks impregnated with mandarin juice. Plasma aminotransferases were determined as index of hepatocellular damage and interleukin-6 as measure of inflammation. Plasma and liver carbonyl groups, indicative of protein oxidation, and α-tocopherol as evaluation of antioxidant defense were measured. The modified base 8-hydroxydexyguanosine as indicative of DNA damage was determined in liver. Tamoxifen induced an increase in aminotranferases and both plasma and liver markers of oxidative stress and DNA damage that decrease significantly after apple snack consumption; in most of cases they even reach normal values as those achieved previous to induce the injury with tamoxifen. The effect of the mandarin juice incorporated to the structure of apple mainly accounts for increasing levels of α-tocopherol in plasma and liver. The present study shown the potential protective action of the products made of dehydrated apple either impregnated or not with mandarin juice by vacuum impregnation for counteracting the chemically induced liver injury in rats.