Effects of primary amine-based coatings on microglia internalization of nanogels

Mauri, Emanuele; Veglianese, Pietro; Papa, Simonetta; Rossetti, Arianna; Paola, Massimiliano De; Mariani, Alessandro; Posel, Zbyšek; Posocco, Paola; Sacchetti, Alessandro; Rossi, Filippo

doi:10.1016/j.colsurfb.2019.110574

Cited by 10 publications

(9 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Ng Synthesismentioning

confidence: 99%

“…NG were synthesized according to experimental procedure already used in previous works [24,27,28] (for synthesis details, see Supplementary data): briefly, PEG hydroxyl groups were modified with imidazole moieties using CDI (hereinafter PEG-CDI), and PEI chains decorated with propargyl groups were further functionalized through the copper-catalyzed azide-alkyne Huisgen cycloaddition (CuAAC) reaction using two dyes: RhB or Cy5, both bearing azide moieties (molar ratio PEI: dye 1:0.025) (Figs. S1-S8) [24,27,28]. Two solutions were prepared separately: the first solution was obtained by dissolving PEG-CDI (200 mg, 0.025 mmol) in CH 2 Cl 2 (3 mL), and the second one by dissolving in another vessel PEI conjugated with RhB or Cy5 (0.017 mmol) in distilled water (5 mL).…”

Section: Ng Synthesismentioning

confidence: 99%

“…We developed a specific category of nanogels (NG) with high colloidal stability, biocompatibility, and capacity for loading and releasing compounds [23,24]. These NG were investigated for cellular selectivity, uptake, and drug delivery (Rolipram, able to reduce the RNA transcription of pro-inflammatory molecules [25], was used to demonstrate the modulation capability of the system) by identifying the most promising one for selective astrocytes treatment in vitro and in vivo SCI models.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functionalized nanogel for treating activated astrocytes in spinal cord injury

Papa

Veneruso

Mauri

et al. 2021

Journal of Controlled Release

Self Cite

View full text Add to dashboard Cite

Section: Ng Synthesismentioning

confidence: 99%

Section: Ng Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Functionalized nanogel for treating activated astrocytes in spinal cord injury

Papa

Veneruso

Mauri

et al. 2021

Journal of Controlled Release

Self Cite

View full text Add to dashboard Cite

“…Furthermore, it was shown that a high amount of polyethylene glycol coating (obtained in carboxyl systems) reduced the microglia internalization, while a smaller amount (obtained in imidazole systems) guaranteed higher microglia uptake compare to nanogels without coatings. In a more recent work (Mauri et al 2020), they developed a coating strategy through primary amines, in order to reduce microglia internalization. Nanogels were designed following two different routes: direct grafting of aliphatic primary amines and linkage of the -NH 2 modified polyethylene glycol on the nanogel surface.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Functionalization of polymers and nanomaterials for water treatment, food packaging, textile and biomedical applications: a review

et al. 2020

Self Cite

View full text Add to dashboard Cite

The inert nature of most commercial polymers and nanomaterials results in limitations of applications in various industrial fields. This can be solved by surface modifications to improve physicochemical and biological properties, such as adhesion, printability, wetting and biocompatibility. Polymer functionalization allows to graft specific moieties and conjugate molecules that improve material performances. In the last decades, several approaches have been designed in the industry and academia to graft functional groups on surfaces. Here, we review surface decoration of polymers and nanomaterials, with focus on major industrial applications in the medical field, textile industry, water treatment and food packaging. We discuss the advantages and challenges of polymer functionalization. More knowledge is needed on the biology behind cell–polymer interactions, nanosafety and manufacturing at the industrial scale.

show abstract

“…In this direction, Papa and coworkers introduced an amine group to PEG-PEI nanogels to increase astrocytes selectivity for spinal cord injury treatment [ 24 ]. This strategy can be also extremely useful to reduce macrophage uptake that is undesired in many medical problems [ 25 ]. Details about this strategy can be found in a recent review published by our group [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of the Core Formulation on Features and Drug Delivery Ability of Carbamate-Based Nanogels

Pinelli

Pizzetti

Ortolà

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

In the last years, nanogels have emerged as one of the most promising classes of novel drug delivery vehicles since they can be employed in multiple fields, such as various therapeutics or diagnostics, and with different classes of compounds and active molecules. Their features, such as a high volume to surface ratio, excellent drug loading and release ability, as well as biocompatibility and tunable behavior, are unique, and, nowadays, great efforts are made to develop new formulations that can be employed in a wider range of applications. Polyethylene glycol (PEG)-polyethylenimine (PEI) nanogels probably represent the baseline of this class of biomaterials and they are still largely employed and studied. In any way, the possibility to exploit new core formulations for nanogels is certainly very interesting in order to understand the influence of different polymer chains on the final properties of the system. In this research, we explore and make a comparison between PEG-PEI nanogels and two other different formulations: pluronic F127-PEI nanogels and PEG-Jeffamine nanogels. We propose nanogels synthesis methods, their chemical and physical characterization, as well as their stability analysis, and we focus on the different drug delivery ability that these structures exhibit working with different typologies of drug mimetics.

show abstract

Effects of primary amine-based coatings on microglia internalization of nanogels

Cited by 10 publications

References 52 publications

Functionalized nanogel for treating activated astrocytes in spinal cord injury

Functionalized nanogel for treating activated astrocytes in spinal cord injury

Functionalization of polymers and nanomaterials for water treatment, food packaging, textile and biomedical applications: a review

Influence of the Core Formulation on Features and Drug Delivery Ability of Carbamate-Based Nanogels

Contact Info

Product

Resources

About