Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts

Wang, Yonggang; Zhu, Fang; Chen, Jie; Han, Fusen; Wang, He-Yao

doi:10.1248/bpb.32.389

Cited by 9 publications

(3 citation statements)

References 37 publications

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“…The extent of oxidation can therefore be measured by the fluorescence intensity with and without the presence of antioxidative compounds (Wolfe & Liu, 2007). Several studies have used this technique to measure the inhibition of intracellular ROS generation by peptides like PR, which is derived from a protamine hydrolysate (Wang, Zhu, Chen, Han, & Wang, 2009), and SS-31, which is a synthetically made cell-penetrating peptide (Zhao, Luo, Giannelli, & Szeto, 2005 …”

Section: In Vitro Biological Assaysmentioning

confidence: 99%

Food-derived peptidic antioxidants: A review of their production, assessment, and potential applications

Samaranayaka

Li‐Chan

2011

Journal of Functional Foods

672

376

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Section: In Vitro Biological Assaysmentioning

confidence: 99%

Food-derived peptidic antioxidants: A review of their production, assessment, and potential applications

Samaranayaka

Li‐Chan

2011

Journal of Functional Foods

672

376

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“…In the presence of an antioxidant, the fluorescence intensity is reduced because the formation of DCF is inhibited ( Figure 5). This methodology has been used to evaluate the antioxidant activity of a novel dipeptide (Pro-Arg) derived from protamine hydrolysis (Wang et al 2009). Casein phosphopeptides (CPPs) possess a potential cytoprotective effect against H 2 O 2 -induced oxidative stress, by preserving cells and enzyme-mediated antioxidant systems.…”

Section: Antioxidant Activity Of Peptidesmentioning

confidence: 99%

Functional role of bioactive peptides with special reference to cheeses

Rafiq¹,

Gulzar²,

Sameen

et al. 2020

Int J of Dairy Tech

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The bioactive peptides released from cheeses have important physiological functions such as angiotensinogen converting enzyme (ACE)‐ inhibition, anti‐ oxidant, anti‐ thrombotic, anti‐ microbial, anti‐ cancer, and anti‐ inflammatory. These bioactive peptides can be produced from precursor milk protein during processing, enzymatic hydrolysis, microbial fermentation and gastrointestinal digestion.

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“…For example, prolyl hydroxyproline (Pro-Hyp) stimulates the growth of fibroblasts from mouse skin (11). Pro-Arg can protect against oxidative stress/damage and H 2 O 2 -induced human diploid fibroblast cell death (13). Furthermore, the lactotripeptides Ile-Pro-Pro and Val-Pro-Pro exhibit angiotensin I-converting enzyme-inhibiting activity (9).…”

mentioning

confidence: 99%

Prolyl Aminopeptidase from Streptomyces thermoluteus subsp. fuscus Strain NBRC14270 and Synthesis of Proline-Containing Peptides by Its S144C Variant

Yamamoto¹,

Usuki²,

Iwabuchi³

et al. 2010

Appl Environ Microbiol

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We specifically examined an exopeptidase, prolyl aminopeptidase (PAP), as a target for synthesis of prolinecontaining peptides. A PAP from Streptomyces thermoluteus subsp. fuscus NBRC14270 (PAP14270) was obtained using sequence-based screening. From PAP14270, 144Ser was replaced by Cys (scPAP14270) to give aminolysis activity. In contrast to wild-type PAP14270, scPAP14270 produced a polymer of proline benzyl ester and cyclo[Pro-Pro]. The product mass was confirmed using liquid chromatography-mass spectrometry (LC/MS). Several factors affecting the reaction, such as the pH, concentration of the substrate, and reaction time, were measured to determine their effects. Furthermore, a correlation was found between substrate specificity in proline peptide synthesis and the log D value of acyl acceptors in aminolysis catalyzed by scPAP14270. Results showed that dipeptide synthesis proceeded in a weakly acidic environment and that cyclization and polymerization occurred under alkaline conditions. Furthermore, results suggest that almost all amino acid esters whose log D value is greater than 0, except hydroxyproline benzyl ester (Hyp-OBzl), can be recognized as acyl acceptors. These findings support the use of PAPs as a tool for production of physiologically active proline peptides.

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Effects of Pro-Arg, a Novel Dipeptide Derived from Protamine Hydrolysate on H2O2-Induced Oxidative Stress in Human Diploid Fibroblasts

Cited by 9 publications

References 37 publications

Food-derived peptidic antioxidants: A review of their production, assessment, and potential applications

Food-derived peptidic antioxidants: A review of their production, assessment, and potential applications

Functional role of bioactive peptides with special reference to cheeses

Prolyl Aminopeptidase from Streptomyces thermoluteus subsp. fuscus Strain NBRC14270 and Synthesis of Proline-Containing Peptides by Its S144C Variant

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