Effects of probiotics Bacillus cereus NY5 and Alcaligenes faecalis Y311 used as water additives on the microbiota and immune enzyme activities in three mucosal tissues in Nile tilapia Oreochromis niloticus reared in outdoor tanks

Wang, Miao; Yi, Ming; Lu, Maixin; Gao, Fengying; Liu, Zhigang; Huang, Qiubiao; Li, Qingyong; Zhu, Dexing

doi:10.1016/j.aqrep.2020.100309

Cited by 45 publications

(31 citation statements)

References 57 publications

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“…Besides enhanced growth performance, L. paracasei l61-27b significantly stimulated skin mucus immunity. The immune response of skin mucus was tested by lysozyme and peroxidase in the present study, which showed similar results with earlier studies in Nile tilapia (Kuebutornye et al, 2020;Van Doan et al, 2018;M. Wang, Yi, et al, 2020); roach (Rutilus rutilus caspicus) fingerlings (Tarkhani et al, 2020) and juvenile Siberian sturgeon (Acipenser baerii) (Ghodrati et al, 2021).…”

Section: Discussionsupporting

confidence: 88%

Modulation of growth, skin mucus and serum immunities, and disease resistance of Nile tilapia fed host‐associated probiotic (Lactobacillus paracasei l61‐27b)

Lumsangkul

Jaturasitha

Meidong

et al. 2021

Aquaculture Nutrition

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With the increased population number, it is necessary to develop traditional food production sources with more sustainable methods to afford healthy food for humanity (Shah & Mraz, 2020). The aquaculture sector is not away from this strategy, which is the primary source of low-cost protein food (Adesogan et al., 2020). It is also thought to be the speediest-growing method of producing animal protein source for mankind utilization. Tilapia is cultivated in over 100 nations and seems to be the second-most farmed fish in terms of production (Prabu et al., 2019). Given the importance of Nile tilapia (Oreochromis niloticus) as the foremost candidate for intensive

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Section: Discussionsupporting

confidence: 88%

Modulation of growth, skin mucus and serum immunities, and disease resistance of Nile tilapia fed host‐associated probiotic (Lactobacillus paracasei l61‐27b)

Lumsangkul

Jaturasitha

Meidong

et al. 2021

Aquaculture Nutrition

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“…This inference was confirmed by our results on intestinal metabolites, i.e., Undibacterium was positively correlated with 9,12-(Z,Z)-octadecadienoic acid, glycerol, and methyl-inositol. Escherichia - Shigella is a common opportunistic pathogen in the gastrointestinal tract of fish [ 18 ]. Dietary changes resulted in the decrease of Escherichia - Shigella in the gut mucosa of the bullfrog, accompanied by a profound reduction of growth performance and immune function [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, the relative abundance of Syntrophus was negatively correlated with N-acetylglutamic acid, 4-aminobutyric acid, octadecanoic acid, and arachidonic acid but positively correlated with cysteine, aspartic acid, and methionine. Some metabolites were not significantly correlated with microorganisms because enzymes that produced these metabolites were encoded in organisms [ 18 ]. Interestingly, the relative abundance values of some potentially beneficial bacteria, including Undibacterium , Crenothrix , and Cetobacterium were positively correlated with most intestinal metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Host-associated probiotics have a greater chance of competing with resident microorganisms, thus more effectively colonizing the host [15]. Therefore, the use of hostassociated probiotics for disease prevention has become a consensus in aquaculture [15], and related studies have been conducted in tilapia [16][17][18]. To better screen host-associated probiotics, the gill and gastrointestinal mucosa-specific microbiota of farmed tilapia need to be further studied.…”

Section: Introductionmentioning

confidence: 99%

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Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

Zhang

Lin

et al. 2021

Microorganisms

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The gill and gastrointestinal tract are primary entry routes for pathogens. The symbiotic microbiota are essential to the health, nutrition and disease of fish. Though the intestinal microbiota of Nile tilapia (Oreochromis niloticus) has been extensively studied, information on the mucosa-associated microbiota of this species, especially the gill and gastrointestinal mucosa-associated microbiota, is lacking. This study aimed to characterize the gill and gastrointestinal mucosa- and digesta-associated microbiota, as well as the intestinal metabolite profiles in the New Genetically Improved Farmed Tilapia (NEW GIFT) strain of farmed adult Nile tilapia by high-throughput sequencing and gas chromatography/mass spectrometry metabolomics. The diversity, structure, composition, and predicted function of gastrointestinal microbiota were significantly different across gastrointestinal regions and sample types (Welch t-test; p < 0.05). By comparing the mucosa- and digesta-associated microbiota, linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed that Pelomonas, Ralstoniapickettii, Comamonadaceae, and Staphylococcus were significantly enriched in the mucosa-associated microbiota, whereas many bacterial taxa were significantly enriched in the digesta-associated microbiota, including Chitinophagaceae, Cetobacterium, CandidatusCompetibacter, Methyloparacoccus, and chloroplast (LDA score > 3.5). Furthermore, Undibacterium, Escherichia–Shigella, Paeniclostridium, and Cetobacterium were dominant in the intestinal contents and mucosae, whereas Sphingomonasaquatilis and Roseomonasgilardii were commonly found in the gill and stomach mucosae. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) analysis revealed that the predictive function of digesta-associated microbiota significantly differed from that of mucosa-associated microbiota (R = 0.8152, p = 0.0001). In addition, our results showed a significant interdependence between specific intestinal microbes and metabolites. Notably, the relative abundance values of several potentially beneficial microbes, including Undibacterium, Crenothrix, and Cetobacterium, were positively correlated with most intestinal metabolites, whereas the relative abundance values of some potential opportunistic pathogens, including Acinetobacter, Mycobacterium, Escherichia–Shigella, Paeniclostridium, Aeromonas, and Clostridiumsensustricto 1, were negatively correlated with most intestinal metabolites. This study revealed the characteristics of gill and gastrointestinal mucosa-associated and digesta-associated microbiota of farmed Nile tilapia and identified a close correlation between intestinal microbes and metabolites. The results serve as a basis for the effective application of targeted probiotics or prebiotics in the diet to regulate the nutrition and health of farmed tilapia.

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“…In the body of fish, there are significant deviations in autoflora caused by such phenomena as violation of conditions of keeping and feeding, constant stress effects of various etiologies, contributing to negative shifts in microflora [1]. Against the background of a deficiency of normal flora, the normal relations between obligate intestinal microorganisms are disrupted, causing an imbalance between normo-and opportunistic microorganisms and dysbacteriosis [9][10][11][12][13][14][15]. In this regard, the implementation of preventive measures, including the use of immunobiological drugs in order to form normobiosis and colonization resistance, is of no small importance.…”

Section: Introductionmentioning

confidence: 99%

Influence of modern immunobiological preparations based on live bacteria in compound feeds on fish-biological and physiological-biochemical parameters of sturgeon

et al. 2020

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The efficiency of modern aquaculture largely depends on the properties of the feed used. High-quality compound feed should be used by the body to the maximum extent, stimulate its growth and development, and contribute to the environmental safety of the food products produced. To obtain additional gains in body weight, increase the digestibility and assimilability of feed, stimulate nonspecific immunity and save feed costs, immunobiological preparations based on live bacteria are currently introduced into the composition of feed for animals, including fish. One of the most promising immunobiological preparations based on live bacteria is the probiotic "Ferm-KM" containing phyto-microsorbent particles, live bacillus cells and metabolites: strains Bacillus subtillis, Bacillus licheniformis, complex of lactic acid bacteria and products of their metabolism. The article presents the results of the use of the probiotic "Ferm-KM" in the composition of the production compound feed for sturgeon fish. It was found that when two-year-old Russian sturgeon are grown on a production compound feed with the addition of a probiotic, the fish-breeding and biological parameters of fish increase and the physiological and biochemical parameters of blood improve. The optimal rate of introducing probiotic into the composition of the production compound feed for commercial growing of sturgeon fish is 5 g / kg of compound feed. The effectiveness of introducing a probiotic preparation "Ferm-KM" with an immunomodulatory effect into the composition of compound feed for sturgeon fish has been proved, which helps to increase the linear-weight gain of fish and improve blood parameters.*

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Effects of probiotics Bacillus cereus NY5 and Alcaligenes faecalis Y311 used as water additives on the microbiota and immune enzyme activities in three mucosal tissues in Nile tilapia Oreochromis niloticus reared in outdoor tanks

Cited by 45 publications

References 57 publications

Modulation of growth, skin mucus and serum immunities, and disease resistance of Nile tilapia fed host‐associated probiotic (Lactobacillus paracasei l61‐27b)

Modulation of growth, skin mucus and serum immunities, and disease resistance of Nile tilapia fed host‐associated probiotic (Lactobacillus paracasei l61‐27b)

Taxonomic and Functional Characteristics of the Gill and Gastrointestinal Microbiota and Its Correlation with Intestinal Metabolites in NEW GIFT Strain of Farmed Adult Nile Tilapia (Oreochromis niloticus)

Influence of modern immunobiological preparations based on live bacteria in compound feeds on fish-biological and physiological-biochemical parameters of sturgeon

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