Effects of Probiotics on the Localization of T Cell Subsets in the Intestine of Broiler Chicks

Huang, Anqi; Shibata, Eri; Nishimura, H.; Igarashi, Yasumi; Isobe, Naoki; Yoshimura, Y.

doi:10.2141/jpsa.0120134

Cited by 10 publications

(9 citation statements)

References 17 publications

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“…(Figs 5 and 6). Huang et al (2013) reported that probiotics enhanced the formation of pool of CD8 + T cells in the intestinal mucosa and also correlated with the results of Haghighi et al (2008) who reported that the elevation of CD8 + T cells density by probiotics treatment.…”

Section: Becton Dickinsonsupporting

confidence: 76%

Immunomodulatory Potential of Microencapsulated Multispecies Probiotic Consortium in Newcastle Disease Virus Vaccinated Chicken

Radhika

Subriya

Manjari

et al. 2019

IJAR

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A study was conducted to investigate the effect of microencapsulated probiotic consortium containing Lactobacillus plantarum, Enterococcus faecium, Enterococcus hirae, Pediococcus acidilactici and Weissella paramesenteroides on immune modulation in Newcastle Disease vaccinated chicken. Humoral immune response was assessed by ELISA. Th1, Th2 cytokine response and cell mediated immune response were assessed by using Real time PCR and flow cytometry respectively. Results indicated significantly (p less than 0.01) higher antibody titer and also higher IL-2, IL-12, IL-4 and IL-10 cytokine expression in NDV vaccinated multispecies probiotic fed group compared to commercial probiotic fed and control groups. It was also observed that higher proportions of Bu1A (B cell receptor) and CD3 (T cell receptor) positive cells in chicken fed with multispecies probiotic supplementation. Hence, it is concluded that multispecies probiotic played an important role in augmenting humoral and cell mediated immune response against NDV.

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Section: Becton Dickinsonsupporting

confidence: 76%

Immunomodulatory Potential of Microencapsulated Multispecies Probiotic Consortium in Newcastle Disease Virus Vaccinated Chicken

Radhika

Subriya

Manjari

et al. 2019

IJAR

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“…Recent studies demonstrated that probiotics can improve T cell subsets through regulation of gut microbiota ( 48 ). Huang et al ( 49 ) observed that combined probiotics consisting of Streptococcus faecalis, Clostridium buthricum , and Bacillus mesentericus can induce T cell subsets in the intestine of broiler chicks. These probiotics mainly cause an influx of CD8 + T cells into the intestinal mucosa; this influx may enhance intestinal immunity.…”

Section: Discussionmentioning

confidence: 99%

Probiotic Enhanced Intestinal Immunity in Broilers against Subclinical Necrotic Enteritis

Wang

Qing

et al. 2017

Front. Immunol.

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Along with banning of antibiotics, necrotic enteritis (NE), especially subclinical NE (SNE) whereby no clinical signs are present in chicks, has become one of the most threatening problems in poultry industry. Therefore, increasing attention has been focused on research and application of effective probiotic strains, as an alternative to antibiotics, to prevent SNE in broilers. In the present study, we evaluated the effects of Lactobacillus johnsonii BS15 on the prevention of SNE in broilers. Specifically, assessment determined the growth performance and indexes related to intestinal mucosal immunity in the ileum and cecal tonsil of broilers. A total of 300 1-day-old Cobb 500 chicks were randomly distributed into the following 5 groups: control group (fed with basal diet + de Man, Rogosa, and Sharpe liquid medium [normal diet]), SNE group (normal diet), BS15 group (basal diet + 1 × 106 colony-forming units BS15/g as fed [BS15 diet]), treatment group (normal diet [days 1–28] + BS15 diet [days 29–42]), and prevention group (BS15 diet [days 1–28] + normal diet [days 29–42]) throughout a 42-day experimental period. SNE infection was treated for all chicks in the SNE, BS15, treatment, and prevention groups. The present results demonstrated that BS15 supplementation of feeds in BS15 and prevention groups exerted a positive effect on preventing negative influences on growth performance; these negative influences included low body weight gain and increased feed conversion ratio caused by SNE. Although no changes were detected in all determined indexes in cecal tonsils, BS15-treated broilers were free from SNE-caused damage in villi in the ileum. BS15 inhibited SNE-caused decrease in immunoglobulins in the ileum. In the lamina propria of ileum, T cell subsets of lymphocytes influenced by SNE were also controlled by BS15. BS15 affected antioxidant abilities of the ileum and controlled SNE-induced mitochondrion-mediated apoptosis by positively changing contents and/or mRNA expression levels of apoptosis-related proteins. These findings indicate that BS15 supplementation may prevent SNE-affected growth decline mainly through enhancing intestinal immunity in broilers.

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“…Huang et al reported that in chicken there were only a few CD4 + T cells in the intestine while CD8 + T cells predominate [ 21 ]. Similar to Huang’s report, we also found that CD4 + T cells represented minor proportions among the intestinal lymphocyte subpopulations since CD4 + /CD8 + ratios both in IELs and LPLs were less than 1.0 in all samples of both groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of Aflatoxin B1 on T-Cell Subsets and mRNA Expression of Cytokines in the Intestine of Broilers

Jiang¹,

Peng

Fang

et al. 2015

IJMS

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This study was conducted to investigate the effects of aflatoxin B1 (AFB1) on T-cell subsets and mRNA expression of cytokines in the small intestine of broilers. One hundred and fifty-six one-day-old healthy Cobb broilers were randomly divided into control group (0 mg/kg AFB1) and AFB1 group (0.6 mg/kg AFB1) with three replicates per group and 26 birds per replicate for 21 days, respectively. At 7, 14, and 21 days of age, the duodenum, jejunum and ileum were sampled for analyzing T cell subsets (CD3+, CD3+CD4+ and CD3+CD8+) by flow cytometry as well as IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α mRNA expression by qRT-PCR. The percentages of T-cells in the intra-epithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) of duodenum, jejunum and ileum in the AFB1 group showed a decreased tendency in comparison to the control group. The mRNA expression of cytokines in the three intestinal segments in the AFB1 group presented a general decline compared with the control groups. Our data demonstrated that 0.6 mg/kg AFB1 in the broilers diet could reduce the percentages of T-cell subsets and the expression level of cytokine mRNA in the small intestine, implying that the immune function of the intestinal mucosa might be affected. The reduction of cytokines mRNA expression may be closely associated with the decreased proportions of T cells subsets induced by AFB1.

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Effects of Probiotics on the Localization of T Cell Subsets in the Intestine of Broiler Chicks

Cited by 10 publications

References 17 publications

Immunomodulatory Potential of Microencapsulated Multispecies Probiotic Consortium in Newcastle Disease Virus Vaccinated Chicken

Immunomodulatory Potential of Microencapsulated Multispecies Probiotic Consortium in Newcastle Disease Virus Vaccinated Chicken

Probiotic Enhanced Intestinal Immunity in Broilers against Subclinical Necrotic Enteritis

Effects of Aflatoxin B1 on T-Cell Subsets and mRNA Expression of Cytokines in the Intestine of Broilers

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