Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep

González-Bulnes, Antonio; Veiga-López, Almudena; García, Pilar; García-García, R.M.; Ariznavarreta, C.; Sánchez, María Angeles Moro; Tresguerres, J.A.F.; Cocero, M.J.; Flores, Juana M.

doi:10.1016/j.theriogenology.2004.10.013

Cited by 101 publications

(71 citation statements)

References 36 publications

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“…A reduced fertility after progestagen treatment compared with natural estrus has already been reported [21,22]. Possible explanations for the reduction of fertility in progestagen-treated animals are alterations in final follicle growth [23] and ovulation [22][23][24] as well as impairment of sperm transport and survival in the female reproductive tract, reducing the number of fertilized ova [25,26]. When comparing the percentage of ewes lambing to the first estrus after progestagen treatment in this investigation with that of a study [16] performed the previous year with the same flock, the results are almost equal (69.4% vs. 75%).…”

Section: Discussionmentioning

confidence: 83%

Reproductive performance of Lacaune dairy sheep exposed to artificial long days followed by natural photoperiod without and with additional progestagen treatment during the nonbreeding season

et al. 2015

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This study compared the reproductive performance of Lacaune dairy ewes exposed to a light program and subsequent male introduction without (n = 36) or with (n = 36) an additional 6-day progestagen treatment during the nonbreeding season. All ewes were exposed to extended day length (16 hours light and 8 hours darkness) for 77 days during winter (December 15 until March 2) followed by increasing natural photoperiod. At the end of the photoperiodic treatment, three blood samples were collected 6 days apart for progesterone (P4) analysis to determine cyclic activity. One half of the ewes were additionally subjected to a 6-day progestagen treatment in combination with PGF2 and eCG at insert withdrawal. Rams fitted with marking harnesses were introduced to females for 45 days and marked ewes recorded. Ewes exposed to the light program only were joined 40 days after the end of photoperiodic treatment, and ewes with additional progestagen treatment were joined 1 day after insert removal (40-44 days after the end of photostimulation). Lambing data were recorded and fertility (percentage of ewes lambing, lambing rate, and litter size) assessed to the first service period and overall. Mean serum P4 concentrations were similarly (P > 0.05) low in both groups (0.4-0.7 ng/mL vs. 0.4-0.6 ng/mL). On the basis of elevated P4 levels (>1 ng/mL), evidence of luteal activity was found in 27.8% of the ewes at the end of the light program. Estrus response was equally high (97.2%) and estrus distribution highly synchronized in progestagen-treated ewes (91.7% within 4 days). In ewes exposed to the light program only, estrous activity was recorded within 4 days (six ewes), from Day 8 to Day 17 (17 ewes) and from Day 19 to Day 25 (12 ewes) after joining. The percentage of ewes that lambed to the first service period was higher (P < 0.05) in ewes exposed to the light program only than that in the group additionally treated with progestagen/PGF2 /eCG (94.4% vs. 69.4%). Overall, the percentage of lambing ewes was similar in both groups (97.2% and 94.4%), and lambing rates (1.4-1.9) and litter sizes (1.9-2.1) were high and not influenced (P > 0.05) by the treatment. In conclusion, this study reports that exposition of Lacaune ewes to artificial long days followed by natural day length and male introduction is highly effective to induce fertile estrous activity during the nonbreeding season and offers a reliable and practical alternative to hormonal manipulation for out-of-season breeding in sheep. a b s t r a c tThis study compared the reproductive performance of Lacaune dairy ewes exposed to a light program and subsequent male introduction without (n ¼ 36) or with (n ¼ 36) an additional 6-day progestagen treatment during the nonbreeding season. All ewes were exposed to extended day length (16 hours light and 8 hours darkness) for 77 days during winter (December 15 until March 2) followed by increasing natural photoperiod. At the end of the photoperiodic treatment, three blood samples were collected 6 days apart for progesterone (...

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Section: Discussionmentioning

confidence: 83%

Reproductive performance of Lacaune dairy sheep exposed to artificial long days followed by natural photoperiod without and with additional progestagen treatment during the nonbreeding season

et al. 2015

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“…Secondly, in the present study, the rate of live weight loss during summer time didn't affect changes in IGF-I and estradiol concentrations during the early follicular phase, the ability to re-ovulate after synchronisation with progestagens and the number of ova shed for those animals that attained puberty. In comparison to other breeds of sheep (Gonzalez-Bulnes et al, 2005, Viñoles, 2003, obtained low concentrations of estradiol could be explained by the low age of the animals in this experiment and also by the synchronisation treatment as ovulatory follicles from sheep treated with progestagens show deficiencies in the secretion of estradiol during the preovulatory phase (Gonzalez-Bulnes et al, 2005). With regard to IGF-I concentrations, the absence of differences between ewe lambs in the three classes of live weight over the 42 hrs following sponge removal can be explained by the fact that all the animals ovulated after sponge removal, a finding that is consistent with previous findings by Spicer et al (1993).…”

Section: Discussionmentioning

confidence: 93%

Live Weight Changes and Their Relationship with the Ovarian Status of Maiden Fat-Tailed Barbarine Ewes

Salem¹,

Rekik²,

Hamouda

et al. 2010

Jour. Agri. & Mar. Scie.

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The current study assessed the effect of the pattern of live weight change on the ovarian function of maiden Barbarine ewes at approximately 1 year of age. For this purpose, a total of 171 weaned ewe lambs (mean live weight ± s.d. 34.7±3.07 kg and mean age ± SD 196±10 days at weaning) were selected for the experiment. Adjustment of live weight variation was used. Based on the slope of the curve, animals were grouped into three classes LWCI (n=46), LWCII (n=91) and LWCIII (n=34) with live weight loss being highest in LWCI and lowest in LWCIII. Following laparoscopy at 13 months of age, the proportion of ewe lambs found cycling in LWCIII (85.3%) was higher in comparison to animals in LWCI (43.4%; P<0.001) and tended to be superior to those in LWCII (61.5%; P<0.05). Following synchronisation with progestagen of the females found cycling, levels of plasma IGF-I concentrations between 6 and 42 hrs after removal of sponges were not significantly different between the three classes of live weight, and respectively averaged 94.2, 90.8 and 89.8 µg/l for LWCI, LWCII and LWCIII females. Levels of estradiol were also not significantly different between the three groups (0.73, 0.70 and 0.67 pg/ml for LWCI, LWCII and LWCIII ewe lambs, respectively). It was concluded that, in low input systems of semi-arid and arid Tunisia, mating ewe lambs at the approximate age of 1 year is likely to lead to depressed reproductive performance particularly when the summer live weight-loss is elevated.

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“…A melhor resposta ovariana pode-se esperar quando as fêmeas são tratadas no início e final da fase luteal. Assim, o início rápido do estro e grande quantidade de animais ovulando são esperados quando as fêmeas são tratadas ao início da fase luteal (González-Bulnes et al, 2005). Em fêmeas ovinas é conhecido que as doses de prostaglandina são efetivas desde o terceiro dia depois da ovulação (Ali et al, 2009).…”

Section: Resultsunclassified

Efeito do tempo da aplicação de prostaglandina na resposta folicular em ovelhas durante o ciclo estral

Uribe-Velásquez

Souza²,

Osório

2011

R. Bras. Zootec.

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RESUMO -Avaliou-se a resposta ovariana, usando ultrassonografia transretal, à aplicação de duas injeções de prostaglandina (PGF 2α ) em diferentes intervalos. Foram utilizadas 14 fêmeas ovinas da raça Bergamácia durante a estação reprodutiva, sendo que todas receberam uma dose intramuscular de um análogo de PGF 2α . As ovelhas foram distribuídas em dois grupos (n = 7). Em um dos grupos, a PGF 2α foi administrada sete dias (PG7) e, no outro, nove dias (PG9) depois da ovulação, quando o folículo dominante da onda 2 encontrava-se nas fases de crescimento e platô, respectivamente.Examinaram-se os ovários diariamente, utilizando-se um ultrassom Aloka 500, provido de um transdutor linear transretal de 7,5 MHz. A ultrassonografia foi iniciada um dia antes da segunda aplicação de prostaglandina até o dia 10 depois da ovulação em ambos os grupos. Amostras de sangue foram colhidas, via punção jugular, a cada dia, para quantificação de progesterona. As concentrações plasmáticas de progesterona mostraram-se menores no PG7 quando comparado com o PG9, imediatamente antes do tratamento com a PGF 2α (4,10 ± 0,17 vs 4,91 ± 0,29 ng/mL; respectivamente). A média do diâmetro máximo atingido pelo folículo dominante da onda 2 foi maior no grupo tratado PG7 comparado com o PG9 (5,5 ± 0,19 vs 4,29 ± 0,26 mm). Houve efeito do dia nos folículos totais. A quantidade de folículos pequenos (2-2,5 mm) foi maior (4,57 ± 0,78 versus 8,42 ± 1,36) no dia 6, nos grupos PG7 e PG9, respectivamente, mas não foram observadas mudanças nos folículos médios (3-3,5 mm) entre os grupos tratados. A proporção de folículos grandes (4 mm) aumentou depois da luteólise; os valores foram menores nas ovelhas tratadas no PG7 e mantiveram-se estáveis no tempo no PG9.Em ovelhas com rápida e completa luteólise, a ovulação ocorre no folículo dominante da onda 2 em fêmeas tratadas sete ou nove dias após a ovulação.Palavras-chave: folículo dominante, onda folicular, progesterona (P 4 ), sincronização, ultrassonografia Effect of time of prostaglandin administration on follicular response in ewes during oestrous cycleABSTRACT -The purpose of this study was to evaluate ovarian response, using transrectal ultrasonography, to two injections of Prostaglandin F 2α (PGF 2α ) given at different intervals. Fourteen Bergamasca ewes were injected with an i.m. dose of PGF 2α during the breeding season. Ewes were randomly alloted in two groups (n = 7) which received PGF 2α 7 d (PG7) or 9 d (PG9) after ovulation (when the dominant follicle of wave 2 was expected to be in the growth and plateau phase, respectively). Ovaries were examined daily with transrectal ultrasonography Aloka 500 with 7.5 MHz linear array transducer. Ultrasonography started 1 day prior to the second PG injection and ended 10 d after ovulation for both groups.Jugular vein blood samples were collected daily for progesterone assay. Plasma P 4 concentration was lower in PG7 than in PG9, immediately before PGF 2α treatment (4.10 ± 0.17 vs 4.91 ± 0.29 ng/mL, respectively). Mean maximum diameter size attained by the dom...

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Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep

Cited by 101 publications

References 36 publications

Reproductive performance of Lacaune dairy sheep exposed to artificial long days followed by natural photoperiod without and with additional progestagen treatment during the nonbreeding season

Reproductive performance of Lacaune dairy sheep exposed to artificial long days followed by natural photoperiod without and with additional progestagen treatment during the nonbreeding season

Live Weight Changes and Their Relationship with the Ovarian Status of Maiden Fat-Tailed Barbarine Ewes

Efeito do tempo da aplicação de prostaglandina na resposta folicular em ovelhas durante o ciclo estral

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