Effects of progesterone and oestradiol-17  on oxytocininduced release of prostaglandin F-2  in heifers

Lafrance, Martine; Goff, A.K.

doi:10.1530/jrf.0.0820429

Cited by 54 publications

(18 citation statements)

References 29 publications

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“…Oestradiol (Price et al 1995a) and progesterone (Lafrance & Goff 1985) were measured in medium samples in duplicate from day 6 of Experiment 2 without extraction as described. Inter-and intra-assay coefficients of variation (CV) were less than 12% for oestradiol and less than 10% for progesterone.…”

Section: Assaysmentioning

confidence: 99%

Effect of FSH and cell localization on dimeric inhibin-A secretion from bovine granulosa cells in culture

Boudjemaa

Rouillier²,

Bhatia³

et al. 2000

Journal of Endocrinology

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We tested the hypotheses that the secretion of dimeric inhibin-A from cultured bovine granulosa cells is stimulated by FSH, and that antral cells secrete more inhibin-A than do mural cells. Cells from the antral or mural compartment of follicles were cultured in defined medium in two culture systems, and dimeric inhibin-A was measured by two-site ELISA or by Western immunoblotting. In the first culture system, dimeric inhibin-A secretion declined with time in culture, but was significantly (P<0·05) higher from antral than from mural cells (as was total inhibin-measured by RIA). The secretion of dimeric inhibin-A and inhibin-from antral but not mural cells was responsive to FSH. In the second culture system, dimeric inhibin-A secretion increased with time in culture, and was significantly stimulated by FSH, but FSH responsiveness was dependent on the concentrations of insulin in the culture medium. The major forms of inhibin-A secreted had molecular masses of approximately 58, 62, 103-116 and >116 kDa; the 32 kDa form was barely detectable. These different forms were all stimulated by FSH, but the >116 and 62 kDa forms were most responsive to FSH. We conclude that (i) FSH stimulates dimeric inhibin-A secretion from bovine granulosa cells, (ii) the 62 kDa form of inhibin-A may be more responsive to FSH than the 58 kDa form, and (iii) the spatial differentiation of granulosa cell function within the follicle previously observed for oestradiol secretion was also observed for inhibin-and dimeric inhibin-A secretion.

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Section: Assaysmentioning

confidence: 99%

Effect of FSH and cell localization on dimeric inhibin-A secretion from bovine granulosa cells in culture

Boudjemaa

Rouillier²,

Bhatia³

et al. 2000

Journal of Endocrinology

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“…Ovarian steroids also affect uterine PGF 2␣ secretion by acting more slowly to influence uterine secretory responsiveness to oxytocin. Stimulatory effects of progesterone on oxytocin-induced PGF 2␣ secretion are observed after Ն7 days of progesterone exposure [4,5]. These effects appear to be exerted through transcriptional activation of genes that code for hormone receptors [5] and possibly PG synthesizing enzymes [6,7].…”

Section: Introductionmentioning

confidence: 99%

Direct Inhibitory Effect of Progesterone on Oxytocin-Induced Secretion of Prostaglandin F2α from Bovine Endometrial Tissue1

Bogacki

Silvia

Rękawiecki

et al. 2002

Biology of Reproduction

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The effect of progesterone on oxytocin-induced secretion of prostaglandin (PG) F(2alpha) from bovine endometrial tissue explants was examined. Endometrial tissue from the late luteal phase were preincubated for 20 h in control medium. Explants were then treated for 6 h with control medium, oxytocin (10(-7) M), progesterone (10(-5) M), or both hormones. Oxytocin increased the medium concentration of 13,14-dihydro-15-keto-PGF(2alpha), whereas progesterone completely suppressed the stimulatory effect of oxytocin. In experiment 2, isolated endometrial epithelial cells were incubated with progesterone (10(-5) M), oxytocin (10(-7) M), and combinations of these hormones with or without actinomycin D (1 ng/ml). Only oxytocin stimulated secretion of PGF(2alpha), and this response was suppressed by progesterone. Oxytocin induced a rapid increase in intracellular concentrations of Ca(2+) detected within 1 min of exposure of epithelial cells from the same cows. Progesterone pretreatment diminished this response. In experiment 3, direct effects of progesterone (2 nM-20 microM) on binding of (3)H-oxytocin to the membrane preparation from epithelial cells were determined by saturation analysis. Oxytocin binding was suppressed by progesterone at every dosage tested. Progesterone is capable of suppressing the ability of oxytocin to induce endometrial secretion of PGF(2alpha). This effect appears to be mediated through a direct interference in the interaction of oxytocin with its own receptor.

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“…It can be assumed that even less than 20 % of OT may be enough to induce luteolysis. However, to prove that OT affects PGF secretion it was necessary to treat cow with 20-100 IU of OT [13,25]. Moreover, continuous blockade of OT receptors in heifers from day 15 until oestrus did not affect luteolysis compared to control [22].…”

mentioning

confidence: 99%

Uterine secretion of prostaglandin F2α stimulated by different doses of oxytocin and released spontaneously during luteolysis in cattle

Kotwica¹,

Skarzynski²,

Jaroszewski³

et al. 1998

Reprod. Nutr. Dev.

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-The objectives were to determine the involvement of oxytocin (OT) in the stimulation of prostaglandin F2a (PGF) secretion during luteolysis in cattle. On days 16-17 of the oestrous cycle, catheters were inserted into the aorta abdominalis of heifers for OT or saline infusion and into the jugular vein for blood sample collection. The following day, heifers were assigned to one of three experimental groups (Gr): Gr I -10 IU OT (n = 4); Gr II -20 IU OT (n = 4); Gr III -50 IU OT (n = 4). Blood samples were collected every 10 min during a 1-h control period before treatment and every 5-10 min for 2 h after OT treatment. In Gr IV (n = 5), a catheter was inserted into the jugular vein on day 15 of the cycle and blood samples were collected every 15 min for 12 h on days [16][17][18][19]. Plasma concentrations of progesterone, PGF metabolite, 13, 14-dihydro-15-keto-prostaglandin F2a (PGFM) and OT were determined. Within 5 min of infusion of 10 or 20 IU OT, peripheral concentrations of OT (7-12 pg/mL) increased by about 200 and 350-500 pg/mL, respectively. These doses did not affect plasma concentrations of PGFM or progesterone within 1.5 h. Fifty IU of OT increased its maximal peripheral concentration to 1 500 pg/mL, which is over 20 times greater than that observed physiologically. Concentrations of plasma PGFM in Gr III increased from basal concentrations (50-65 pg/mL) to 150-250 pg/mL (P < 0.01) within 10-30 min. During luteolysis, PGFM pulses ranged between 250 and 600 pg/mL on days [16][17][18][19]

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Effects of progesterone and oestradiol-17 on oxytocininduced release of prostaglandin F-2 in heifers

Cited by 54 publications

References 29 publications

Effect of FSH and cell localization on dimeric inhibin-A secretion from bovine granulosa cells in culture

Effect of FSH and cell localization on dimeric inhibin-A secretion from bovine granulosa cells in culture

Direct Inhibitory Effect of Progesterone on Oxytocin-Induced Secretion of Prostaglandin F2α from Bovine Endometrial Tissue1

Uterine secretion of prostaglandin F2α stimulated by different doses of oxytocin and released spontaneously during luteolysis in cattle

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