Effects of proinflammatory cytokines on axonal outgrowth from adult rat lumbar dorsal root ganglia using a novel three-dimensional culture system

Kim, Hyunchul; Caspar, Tyler W.; Shah, Sameer B.; Hsieh, Adam H.

doi:10.1016/j.spinee.2015.03.017

Cited by 13 publications

(10 citation statements)

References 55 publications

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“…[19][20][21][22][23] It has been used in studies of neural regeneration, as a model of peripheral sensory neuron growth for tissue scaffold design, and as a nociceptor model for examining regenerationassociated gene upregulation. The F11 model avoids the need for repeated harvests of lumbar dorsal root ganglia as described by Kim et al 24 in their studies of axonal outgrowth. These cells are derived from a fusion between mouse embryonic neuroblastoma and rat dorsal root ganglion (DRG) neurons.…”

Section: Discussionmentioning

confidence: 99%

Human annulus signaling cues for nerve outgrowth: In vitro studies

Gruber

Hoelscher

Bullock

et al. 2016

Journal Orthopaedic Research

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The relationship between neurotrophins produced by human annulus cells, such as neurotrophin-4 (NT4) and brain-derived neurotrophic factor (BDNF) which function in neurite survival and outgrowth, and nerve ingrowth into the disc remains poorly understood. In this work, we tested F11 neurite growth during exposure to control media, media with added nerve growth factor (NGF), conditioned media (CM) harvested from previous human annulus culture, or co-culture with annulus cells. Co-culture of F11 cells with annulus cells significantly increased media levels of amphiregulin, BDNF, glial-derived neurotrophic factor, and vascular endothelial growth factor compared to levels from in culture of F11 cells alone (p ≤ 0.04). Cell-based assays of neurite growth revealed that BDNF levels present in CM bore a significant (p = 0.01) positive relationship to neurite length and accounted for 38.5% of the change in neurite length. NT4 levels produced during co-culture with annulus cells bore a significant (p = 0.04) positive relationship to neurite length and accounted for 40.9% of the change in length. Statement of clinical significance: In vitro findings point to a potential role of annulus cells related to nerve ingrowth in vivo, and may have relevance in the outer annulus (where cell numbers are high) or in regions where nerves penetrate into annular tears or fissures. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1456-1465, 2016.

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Section: Discussionmentioning

confidence: 99%

Human annulus signaling cues for nerve outgrowth: In vitro studies

Gruber

Hoelscher

Bullock

et al. 2016

Journal Orthopaedic Research

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“…When binding to the receptors, NGF regulates the survival, proliferation, differentiation, and chemotaxis of neurons 35 . Kim et al demonstrated that NGF could promote neurite outgrowth from rat lumbar DRG into three-dimensional collagen hydrogels in vitro 36 . In addition, NGF receptors are also expressed in chondrocyte-like IVD cells, and NGF is involved in the process of matrix turnover during IVDD.…”

Section: Figmentioning

confidence: 99%

The role of structure and function changes of sensory nervous system in intervertebral disc-related low back pain

Zhang

Liu

et al. 2021

Osteoarthritis and Cartilage

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Low back pain (LBP) is a common musculoskeletal symptom, which can be developed in multiple clinical diseases. It is widely recognized that intervertebral disc (IVD) degeneration (IVDD) is one of the leading causes of LBP. However, the pathogenesis of IVD-related LBP is still controversial, and the treatment means are also insufficient to date. In recent decades, the role of structure and function changes of sensory nervous system in the induction and the maintenance of LBP is drawing more and more attention. With the progress of IVDD, IVD cell exhaustion and extracellular matrix degradation result in IVD structural damage, while neovascularization, innervation and inflammatory activation further deteriorate the microenvironment of IVD. New nerve ingrowth into degenerated IVD amplifies the impacts of IVD-derived nociceptive molecules on sensory endings. Moreover, IVDD is usually accompanied with disc herniation, which could injure and inflame affected nerves. Under mechanical and pro-inflammatory stimulation, the pain-transmitting pathway exhibits a sensitized function state and ultimately leads to LBP. Hence, relevant pathogenic factors, such as neurotrophins, ion channels, inflammatory factors, etc., are supposed to serve as promising therapeutic targets for LBP. The purpose of this review is to comprehensively summarize the current evidence on 1) the pathological changes of sensory nervous system during IVDD and their association with LBP, and 2) potential therapeutic strategies for LBP targeting relevant pathogenic factors.

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“…Indeed, Kim and collaborators studied the effect of several growth factors on sensory neurons isolated from adult rats, grown on collagen hydrogels for 7 days (Kim, Caspar, Shah, & Hsieh, 2015). They showed that the neurite length reached 229 ± 102 µm in the absence of NGF.…”

Section: Evaluation Of Neurite Growth On the Hydrogelsmentioning

confidence: 99%

Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications

Santos

Garbay

Pasqua

et al. 2019

Journal of Biotechnology

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Elastin-like polypeptides (ELPs) are biocompatible-engineered polypeptides, with promising interest in tissue engineering due to their intrinsic biological and physical properties, and their ease of production. The IKVAV (Ile-Lys-Val-Ala-Val) laminin-1 sequence has been shown to sustain neuron attachment and growth. In this study, the IKVAV adhesion sequence, or a scrambled VKAIV sequence, were incorporated by genetic engineering in the structure of an ELP, expressed in Escherichia coli and purified. The transition temperatures of the ELP-IKVAV and ELP-VKAIV were determined to be 23 °C. Although the phase transition was fully reversible for ELP-VKAIV, we observed an irreversible aggregation for ELP-IKVAV. The corresponding aggregates shared some characteristics with amyloid-like polypeptides. The two ELPs were then reacted with functionalized polyethylene glycol (PEG) to form hydrogels. These hydrogels were characterized for rheological properties, tested with cultures of rat primary sensory neurons, and implanted subcutaneously in mice for 4 weeks. Sensory neurons cultured on high IKVAV concentration hydrogels (20%) formed longer neurite than those of neurons grown on hydrogels containing the scrambled IKVAV sequence. Finally, in vivo evaluation showed the absence of detectable inflammation. In conclusion, this functionalized ELP-IKVAV biomaterial shows interesting properties for tissue engineering requiring neurotization.

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Effects of proinflammatory cytokines on axonal outgrowth from adult rat lumbar dorsal root ganglia using a novel three-dimensional culture system

Cited by 13 publications

References 55 publications

Human annulus signaling cues for nerve outgrowth: In vitro studies

Human annulus signaling cues for nerve outgrowth: In vitro studies

The role of structure and function changes of sensory nervous system in intervertebral disc-related low back pain

Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications

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