Effects of prolactin and growth hormone on proliferation and survival of cultured trout leucocytes

Yada, Takashi; Misumi, Ichiro; Muto, Kohji; Azuma, Teruo; Schreck, Carl B.

doi:10.1016/j.ygcen.2004.01.003

Cited by 38 publications

(18 citation statements)

References 44 publications

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“…Among the leukocytes, lymphocytes and monocytes are important immune cells mediating specific and non-specific immune response in fish, respectively. Thus, the enhanced leukocytosis in the transgenics supports the finding of Yada et al (2004), who suggested that GH showed stimulatory effects not only on lymphopoiesis, but also on proliferation of other leukocytes in trout (O. mykiss) in vitro, resulting in an increase in the total blood cell number. Under common status, the amount of GH in a fish is not fully saturated, so that the expressive GH production by the transgene has a chance to exert its biological effects on the fish (Donaldson et al, 1979).…”

Section: Discussionsupporting

confidence: 84%

Effects of the “all-fish” growth hormone transgene expression on non-specific immune functions of common carp, Cyprinus carpio L.

Wang

et al. 2006

Aquaculture

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Section: Discussionsupporting

confidence: 84%

Effects of the “all-fish” growth hormone transgene expression on non-specific immune functions of common carp, Cyprinus carpio L.

Wang

et al. 2006

Aquaculture

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“…The nonadherent leukocytes separated from peripheral blood also express GHS-R genes. The nonadherent leukocytes were microscopically identified as 95% lymphocytes (Yada et al 2004b). Lymphocytes seem to be targets of ghrelin in teleost fish, as shown in human lymphocytes (Hattori et al 2001, Poppi et al 2002.…”

Section: Discussionmentioning

confidence: 89%

“…It is well known that GH stimulates immune functions in various vertebrates (Sakai et al 1996a, 1996b, Balm 1997, Clark 1997, Cohen & Kinney 2001, Yada & Nakanishi 2002, Yada et al 2004a, 2004b. In fish, in vivo and in vitro administration of GH enhances specific and nonspecific immune functions, such as phagocytosis by neutrophils and macrophages (Sakai et al 1996a, Balm 1997, Yada & Nakanishi 2002, Yada et al 2004a, 2004b.…”

Section: Introductionmentioning

confidence: 99%

“…In fish, in vivo and in vitro administration of GH enhances specific and nonspecific immune functions, such as phagocytosis by neutrophils and macrophages (Sakai et al 1996a, Balm 1997, Yada & Nakanishi 2002, Yada et al 2004a, 2004b. Extrapituitary expression of GH gene in fish leukocytes suggests autocrine or paracrine action of GH in the modulation of immune functions (Yada & Azuma 2002, Yada & Nakanishi 2002, Yada et al 2005.…”

Section: Introductionmentioning

confidence: 99%

“…GH is known to enhance proliferation of fish lymphocytes (Sakai et al 1996a, 1996b, Yada et al 2004b. However, ghrelin causes inhibition of cell proliferation in the human thyroid tumor cell line (Muccioli et al 2002), although it stimulates the proliferation of prostate cancer cell lines (Jeffery et al 2002).…”

mentioning

confidence: 99%

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Ghrelin stimulates phagocytosis and superoxide production in fish leukocytes

Yada¹,

Kaiya²,

Mutoh³

et al. 2006

Journal of Endocrinology

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To clarify the role of ghrelin in the fish immune system, the in vitro effect of ghrelin was examined in phagocytic leukocytes of rainbow trout (Oncorhynchus mykiss). Administration of trout ghrelin and des-VRQ-trout ghrelin, in which three amino acids are deleted from trout ghrelin, increased superoxide production in zymosanstimulated phagocytic leukocytes from the head kidney. Gene expression of growth hormone (GH) secretagoguereceptor (GHS-R) was detected by RT-PCR in leukocytes. Pretreatment of phagocytic leukocytes with a GHS-R antagonist, [D-Lys 3 ]-GHRP-6, abolished the stimulatory effects of trout ghrelin and des-VRQ-trout ghrelin on superoxide production. Ghrelin increased mRNA levels of superoxide dismutase and GH expressed in trout phagocytic leukocytes. Immunoneutralization of GH by addition of anti-salmon GH serum to the medium blocked the stimulatory effect of ghrelin on superoxide production. These results suggest that ghrelin stimulates phagocytosis in fish leukocytes through a GHS-Rdependent pathway, and also that the effect of ghrelin is mediated, at least in part, by GH secreted by leukocytes.

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Androgen and prolactin manipulation do not induce changes in immunocompetence measures in a fish with male parental care

et al. 2022

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Prolactin and 11‐ketotestosterone (11‐KT) are important reproductive hormones in fishes, which may also influence immunocompetence. The immunocompetence handicap hypothesis states that higher androgen concentrations that support secondary sex traits are traded off against a decrease in immune system function. To test the relationships between these hormones and immunocompetence, we experimentally manipulated 11‐ketotestosterone and prolactin in the freshwater fish, bluegill (Lepomis macrochirus) during parental care using implants that contained either 11‐KT, prolactin, or an inert control. We vaccinated individuals to stimulate the acquired immune response, then measured immunocompetence as the number of granulocytes, lymphocytes and monocytes, and the expression of interleukin 8 in each sample. We did not observe any significant differences in the immune measures among the hormone treatments. Our results indicate that in bluegill, there is no trade‐off between androgens or prolactin and immunocompetence.

show abstract

Effects of prolactin and growth hormone on proliferation and survival of cultured trout leucocytes

Cited by 38 publications

References 44 publications

Effects of the “all-fish” growth hormone transgene expression on non-specific immune functions of common carp, Cyprinus carpio L.

Effects of the “all-fish” growth hormone transgene expression on non-specific immune functions of common carp, Cyprinus carpio L.

Ghrelin stimulates phagocytosis and superoxide production in fish leukocytes

Androgen and prolactin manipulation do not induce changes in immunocompetence measures in a fish with male parental care

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