Effects of prolonged administration of anti-cumulus oophorus antibody on reproduction in mice

Testart, J; Nomé, Françoise

doi:10.1530/jrf.0.0900605

Cited by 5 publications

(1 citation statement)

References 10 publications

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“…It has been demonstrated that oocytes resuming meiosis in vitro are less fertilizable if they have been denuded of their corresponding cumulus cells [42,43] or if they have been exposed to anticumulus or antizonal antibodies [44,45]. Coculture of the oocytes with cumulus or granulosa cells when meiosis takes place improves their fertilizability and development [46,47].…”

Section: Discussionmentioning

confidence: 99%

Failure of Meiotic Competence in Human Oocytes1

Bar‐Ami

Zlotkin

Brandes

et al. 1994

Biology of Reproduction

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The administration of hCG to women undergoing in vitro fertilization and embryo transfer (IVF/ET) results in the meiotic maturation of cumulus-oocyte complexes (COC). Sometimes oocytes being aspirated for IVF/ET fail to resume meiosis in vivo and even after a subsequent 20-h incubation in vitro and are thus defined as meiotic competence failure (MCF) oocytes. The relationship between the proportion of MCF oocytes and other IVF/ET outcomes was studied over 3 years in 703 tested cycles of 487 women. Women yielding one or more MCF oocytes in at least one menstrual cycle represented 8.6% of this population and were defined as MCF women. Cumulus state in the MCF oocyte population was characterized as mature in 57.4 +/- 6.7%, intermediate in 13.9 +/- 4.0%, immature in 24.1 +/- 8.7%, and atretic in 4.6 +/- 2.7%. These values differed significantly, by 0.6-, 2.9-, 7.1-, and 4.6-fold, respectively, as compared to the corresponding COC aspirated from women yielding only meiotically competent (MC) oocytes. In a menstrual cycle yielding both MC and MCF oocytes, the IVF/ET variables were evaluated in the MC oocytes. Thus, in such cases the incidence of fertilization or cleavage and the number of blastomeres per embryo were significantly reduced concomitant with the increase in percentage of MCF oocytes. When the percentage of MCF oocytes was 25% or more, no pregnancy was achieved. Various follicular parameters and serum 17 beta-estradiol (E2) and progesterone (P4) levels were compared in MC and MCF women over the four days preceding day of aspiration.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Failure of Meiotic Competence in Human Oocytes1

Bar‐Ami

Zlotkin

Brandes

et al. 1994

Biology of Reproduction

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Progression of Oocyte Maturation From Metaphase I to Metaphase II is disturbed by previous immunological interference with cumulus cell function

Lefèvre

Testart

1991

J. Exp. Zool.

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The effects of an antibody preparation reacting with preovulatory mouse cumuli oophori (anticumulus Ig) on oocyte maturation in vivo and in vitro were studied. Continuous presence of anticumulus Ig in culture medium did not impair oocyte maturation in vitro. Similarly, no effect on oocyte maturation in vivo was observed when anticumulus Ig was given to females superovulated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) at the time of hCG treatment. However, when administered earlier, anticumulus Ig brought about serious disturbances of oocyte meiotic competence, since only immature oocytes were ovulated after anticumulus Ig injection at the time of PMSG treatment and as much as 70% of the ovulated oocytes were immature when the antibody was applied 24 hr later. Previous absorption of anticumulus Ig with isolated cumulus cells removed the inhibitory effect of this preparation on oocyte meiotic competence to the same extent as absorption with whole cumuli oophori, despite the persistence of a strong reactivity of the cumulus cell-absorbed antibody preparations with the cumulus intercellular matrix. The ability of oocytes obtained from antibody-injected animals to mature in vitro was also considerably impaired when the injection was made at the time of PMSG treatment. In all cases the maturation defect concerned the progression of meiosis from metaphase I to metaphase II, while the ability of oocytes to undergo germinal vesicle breakdown (GVBD) was unchanged.(ABSTRACT TRUNCATED AT 250 WORDS)

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Immunoregulation of mammalian fertility

Murdoch

1994

Life Sciences

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Effects of prolonged administration of anti-cumulus oophorus antibody on reproduction in mice

Cited by 5 publications

References 10 publications

Failure of Meiotic Competence in Human Oocytes1

Failure of Meiotic Competence in Human Oocytes1

Progression of Oocyte Maturation From Metaphase I to Metaphase II is disturbed by previous immunological interference with cumulus cell function

Immunoregulation of mammalian fertility

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