Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells

Báguena-Cervellera, R.; Renau‐Piqueras, Jaime; O'Connor, JE; Grisolı́a, Santiago

doi:10.1007/bf00496816

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…Fluorescence microscopy indicates that in control astrocytes, after incubation in medium containing FITC-con A (100 pg/ml) for 180 min at O X C , a temperature reported to inhibit internalization of the ligand (Baguena-Cervellera et al, 1987;Steinman et al, 1983;Willingham et al, 1979), the labeling was restricted to plasma membrane. Interestingly, in all preparations of 20-day cultures examined, a small population of control cells (5-10%) showed both immature morphology and FITC-con A binding.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Prenatal exposure to ethanol alters plasma membrane glycoproteins of astrocytes during development in primary culture as revealed by concanavalin a binding and 5′‐nucleotidase activity

Renau‐Piqueras

Guerri²,

Burgal³

et al. 1992

Glia

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We have investigated the effect of prenatal exposure to ethanol on the extent of binding and surface distribution of the lectin concanavalin A (con A) on rat cortical astrocytes during the periods of proliferation and differentiation in primary culture. The enzymatic activity of the plasma membrane glycoprotein 5'-nucleotidase was also assessed. The cells were obtained from control fetuses (no exposure to ethanol) and from fetuses prenatally exposed to ethanol. The main findings were: 1) both proliferating and differentiating control astrocytes showed two distinct types of surface con A receptors that could correspond to high- and low-affinity binding sites; 2) the extent of con A binding was greater in mature than in proliferating control cells; 3) the distribution of con A on cell surface components changed with differentiation; 4) the activity of 5'-nucleotidase showed a substantial increment during the period of differentiation; and 5) prenatal exposure to ethanol clearly decreased the ability of astrocytes to bind con A, altered the surface distribution of the receptors for this lectin, and decreased the activity of 5'-nucleotidase. These effects were more marked in proliferating cells. In conclusion, it is shown that the extent of con A labeling and the activity of 5'-nucleotidase in astrocytes are dependent on the stage of cell differentiation and that prenatal exposure to ethanol alters the plasma membrane structure of these cells during development.

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Section: Fluorescence Microscopymentioning

confidence: 99%

Prenatal exposure to ethanol alters plasma membrane glycoproteins of astrocytes during development in primary culture as revealed by concanavalin a binding and 5′‐nucleotidase activity

Renau‐Piqueras

Guerri²,

Burgal³

et al. 1992

Glia

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Acidification and endosome-like compartments in the presynaptic terminals of frog retinal photoreceptors

Sulzer

Holtzman

1989

J Neurocytol

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By using the 'acidotropic' vital dye, Acridine Orange, we have found that the presynaptic terminals of rod and cone photoreceptors in retinas of Rana pipiens maintain a low pH relative to the surrounding medium through an energy dependent mechanism. When this pH is raised, by exposing the retinas to weak bases like ammonium chloride, the terminals exhibit large, membrane-delimited compartments, many of which accumulate endocytic tracers. This effect is partly reversed when the weak bases are removed. We infer that among the acidified structures within the terminals are endocytic compartments with at least some of the characteristics of the endosomes that participate in receptor-mediated endocytosis in other cell types. One role of these structures in the terminals may be in the recycling of synaptic vesicles.

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Prenatal Exposure to Ethanol Alters the Synthesis and Glycosylation of Proteins in Fetal Hepatocytes

Renau‐Piqueras

Sancho‐Tello

Cervellera

et al. 1989

Alcoholism Clin & Exp Res

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In the present work we have studied the effect of prenatal exposure to alcohol on the synthesis, glycosylation, and transport of proteins in fetal hepatocytes isolated from 21-day-old fetuses derived from control and chronic alcoholic rats. Protein synthesis was evaluated both in a cell-free system and in hepatocytes after (35S)methionine and (3H)leucine incorporation, respectively. Glycosylation was assessed using (3H)mannose and (3H)galactose as precursors. Protein synthesis was significantly decreased in treated hepatocytes. In control hepatocytes, quantitative electron microscope autoradiography showed that both (3H)leucine and (3H)mannose incorporation occur first in the rough endoplasmic reticulum (rER). Later the silver grains appeared over the Golgi apparatus, and, finally there was a transport towards the cell periphery. After pulse, silver grains corresponding to (3H)galactose incorporation appeared over the Golgi apparatus. The label then moved to the hepatocyte periphery. Alcohol treated hepatocytes showed a retention of grains over the Golgi apparatus with a diminution in the label at the cell periphery. These results indicate that prenatal exposure to alcohol induces a decrease in the synthesis of proteins in the hepatocyte as well as an alteration in the process of glycosylation and/or transport of secretory proteins.

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Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells

Cited by 3 publications

References 30 publications

Prenatal exposure to ethanol alters plasma membrane glycoproteins of astrocytes during development in primary culture as revealed by concanavalin a binding and 5′‐nucleotidase activity

Prenatal exposure to ethanol alters plasma membrane glycoproteins of astrocytes during development in primary culture as revealed by concanavalin a binding and 5′‐nucleotidase activity

Acidification and endosome-like compartments in the presynaptic terminals of frog retinal photoreceptors

Prenatal Exposure to Ethanol Alters the Synthesis and Glycosylation of Proteins in Fetal Hepatocytes

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