Effects of Propolis on Hypoxanthine-Xanthine Oxidase-Induced Toxicity in Cultivated Human Cells and on Neutrophil Elastase Activity

SUMMARYCryptococcosis is a worldwide disease caused by the etiological agent Cryptococcus neoformans. It affects mainly immunocompromised humans. It is relatively rare in animals only affecting those that have received prolonged antibiotic therapy. The propolis is a resin that can present several biological properties, including antibacterial, antifungal and antiviral activities. The standard strain C. neoformans ATTC 90112 was used to the antifungal evaluation. The tests were realized with propolis ethanol extract (PEE) G12 in concentrations from 0.1 to 1.6 mg mL -1 . The evaluation of MIC and MFC were done according to DUARTE (2002) 5 . The inhibitory effect of PEE G12 on the fungal growing was seen at the concentration of 0.2 mg mL -1 and 1.6 mg mL -1 was considered a fungicidal one.

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“…Other properties, as antifungal, antiviral, antiinflammatory and immuno-stimulating have also been described to this resin 3,10 .…”

Section: Introductionmentioning

confidence: 89%

“…Due to the wide variability of its chemical compositions depending on its origin, the chemical standardization is extremely difficult 3 . Several biological and therapeutic activities have been associated with the presence of flavonoids, aromatic acids and esters.…”

Section: Introductionmentioning

confidence: 99%

The "in vitro" antifungal activity evaluation of propolis G12 ethanol extract on Cryptococcus neoformans

Fernandes

Dias

Ramos

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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“…Endothelial cells as an element of the vascular system are a suitable target of the cytotoxicity by oxidant stress [12,13] . The cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 2 mmol/l L -glutamine (GIBCO, Grand Island, N.Y., USA), penicillin (100 U/ml) and streptomycin (100 mg/ml), maintained at 37 ° C with 5% CO 2 in a humidified atmosphere.…”

Section: Cell Culturementioning

confidence: 99%

The Mechanism of Hyperoside Protection of ECV-304 Cells against tert-Butyl Hydroperoxide-Induced Injury

Li¹,

Yi²,

Gao

et al. 2008

Pharmacology

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The aim of the present study was to investigate the mechanism of hyperoside protecting ECV-304 cells against tertbutyl hydroperoxide (TBHP)-induced injury. ECV-304 cell viability was measured by MTT assay. Cellular morphologic changes were observed using phase contrast microscopy. The genotoxic effects of TBHP and the protective ability of hyperoside were assessed by the Comet test. Lipid peroxidation was measured by HPLC method. The cellular redox status was determined from GSH/GSSG ratios. Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay. Western blot analysis was used to evaluate the levels of cytochrome c, p53, SIRT1, Bax and Bcl-2 expression. The results showed that 128 µmol/l hyperoside could effectively protect TBHP-treated ECV-304 cells from death, increase superoxide dismutase activity and significantly decrease malondialdehyde production. Hyperoside was effective in protecting against the induction of oxidized DNA bases and redox state alterations induced by TBHP. Furthermore, the release of proapoptotic cytochrome c from mitochondria was reduced by hyperoside, which increased the expression of antiapoptotic SIRT1 and inhibited the translocation of Bax from cytoplasm to mitochondria. Taken together, these results indicate that hyperoside is effective in protecting against the oxidative damage induced by TBHP. The mechanism of hyperoside protecting against ECV-304 cell apoptosis by TBHP is related with resuming mitochondrial function and regulating the expression of SIRT1 and Bcl-2 family members.

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“…Desta forma, esta enzima parece ser um importante alvo para a terapia de doenças inflamatórias crônicas (SMEDLY et al, 1986;BEYER, MELZIG, 2005 …”

Section: -Quimiotaxiaunclassified

Efeito da sazonalidade no perfil químico e na atividade antioxidante de Baccharis dracunculifolia (Asteraceae) e ação modulatória desta planta sobre o metabolismo oxidativo de neutrófilos

Figueiredo¹

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Effects of Propolis on Hypoxanthine-Xanthine Oxidase-Induced Toxicity in Cultivated Human Cells and on Neutrophil Elastase Activity

Cited by 15 publications

References 22 publications

The "in vitro" antifungal activity evaluation of propolis G12 ethanol extract on Cryptococcus neoformans

The "in vitro" antifungal activity evaluation of propolis G12 ethanol extract on Cryptococcus neoformans

The Mechanism of Hyperoside Protection of ECV-304 Cells against tert-Butyl Hydroperoxide-Induced Injury

Efeito da sazonalidade no perfil químico e na atividade antioxidante de Baccharis dracunculifolia (Asteraceae) e ação modulatória desta planta sobre o metabolismo oxidativo de neutrófilos

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