Effects of Proportion of Egg Yolk and Preservation Time on Chilled Semen from Indigenous Rams

Rekha, Azizunnesa; Zohara, Begum Fatema; Bari, Farida Yeasmin; Alam, Golam Shahi

doi:10.5176/2345-7880_1.1.3

Cited by 10 publications

(13 citation statements)

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“…The volume, colour, mass activity, sperm motility, viability, concentration, HOST +ve (%) and normal spermatozoa (%) varied from 0.4-1.3 ml, 2-4 (creamy to creamy-grainy), 2-5, 75-92%, 80-95%, 2500-5000×10 6 /ml, 7590% and 75-95%, respectively. Similar semen quality parameters were observed in previous study of the same laboratory (Mahmuda et al, 2015 andAzizunnesa et al, 2014). In the present study the sperm concentration of ram R#6 was significantly higher (4120.5±93.5x 10 6 /ml) compared with ram R#1, R#4 and R#5.…”

Section: Discussionsupporting

confidence: 91%

“…This result is similar with the observation of Mahmuda et al (2015). However, the rate of motility and viability of post thawed semen in the present study were lower than observed by Azizunnesa et al (2014). It is not clear to explain why there is difference from Azizunnesa et al (2014), using the same protocol for freezing.…”

Section: Discussionsupporting

confidence: 89%

“…However, the rate of motility and viability of post thawed semen in the present study were lower than observed by Azizunnesa et al (2014). It is not clear to explain why there is difference from Azizunnesa et al (2014), using the same protocol for freezing. This could be due to climatic condition, weather and season of the study.…”

Section: Discussioncontrasting

confidence: 85%

“…The number of synchronized ewes used was lower than natural ewes. The conception rate (26.7% and 25%) in the present study was similar, higher or lower than other researchers (Olesen, 1993;Azizunnesa et al, 2014 andKumar andNaqvi, 2014).…”

Section: Discussionsupporting

confidence: 79%

“…Conception rates with fresh semen are good (65 to 75%) while with frozen semen (10 to 30%) (Olesen, 1993;Gordon, 1997). In Bangladesh, some works have been performed on reproductive performances, estrus synchronization protocol, protocols for semen preservation, AI with chilled and frozen semen in DSO, BAU research station (Azizunnesa et al, 2014(Azizunnesa et al, , 2016. However, about the use of AI is limited to research level with some initiation at farm level.…”

Section: Introductionmentioning

confidence: 99%

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Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

Mansur

Alam

Jha

et al. 2018

Asian J. Med. Biol. Res

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This study was undertaken to study the AI conception rate using frozen semen at field level. Five farms in Mymensingh, Bangladesh were selected for AI Trial in field ewes. Four rams were selected for semen collection, evaluation, and frozen semen production and further to study conception rate followed by intracervical AI in both natural and synchronized ewes. Conception rate were confirmed by non-returned rate and ultrasound scanning at 30-40 days of post insemination. The volume, colour, mass activity, sperm motility, viability, concentration, HOST +ve (%) and normal spermatozoa percentages were 0.8±0.3 ml, 3.9±0.3, 4.4±0.6, 81.3±5.0%, 90.0±4.0%, 3519.0±545.6x10 6 /ml, 87.4±3.3% and 85.6±1.8%, respectively. The sperm concentration of ram R#6 was significantly higher (P<0.05) (4120.5±93.5x10 6 /ml) compared with other rams. The mean motility and viability of pre-dilution, 120 minutes of addition of Part-A, 240 minutes of addition of Part-B and post-thaw were (83.8±4.8%, 81.3±2.5%, 80.0±4.1% and 41.3±9.5%) and (93.3±1.0%, 90.0±1.4%, 88.8±1.0% and 58.3±8.7%), respectively. There were no significant difference (P˃0.05) between pre-dilution and post-dilution sperm motility and viability percentage however, post-thaw sperm motility and viability significantly (P<0.05) decreased compare with the motility and viability of pre-dilution and post-dilution values. Motility and viability percentages of frozen semen did not decrease significantly (P> 0.05) with the increase of preservation time. The mean motility and viability at 24 hrs, day 7, day 15 and day 30 were 41.3±9. 5%, 41.5±8.5%, 41.8±9.9% and 40.5±10.2%; and 59.0±10.1%, 58.5±7.7%, 59.0±8.8% and 57.8±8.3%, respectively. The conception rates in natural and synchronized estrous were 26.7% and 25%, respectively. There was no significant difference in conception rates between the natural and synchronized oestrous in field level. However, the present non-return rate and conception rate indicate the suitability of produced frozen semen application in the field level.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 85%

Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

Mansur

Alam

Jha

et al. 2018

Asian J. Med. Biol. Res

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Examining the relationship between progesterone, blood metabolism profile and vaginal smears in lactating Friesian Holstein cows

Hudaya,

Widayati,

Sitaresmi

2024

International Conference on Organic and Applied Chemistry (Icoac) 2022

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Cryopreservation Techniques for Ram Sperm

Saha

Asaduzzaman

Bari

2022

Veterinary Medicine International

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Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire’s death. However, the technique of cryopreservation might damage sperm and limit their activity. Several cryobiological investigations have reported that the integrity of the sperm membrane is frequently involved in the physical and biological elements that affect sperm survival at low temperatures during the cryopreservation process. However, successful cryopreservation of ram sperm is still a work in progress because a considerable percentage of sperm do not survive the freezing and thawing process. Sperms are destroyed during cryopreservation of semen due to varying concentrations of cryoprotective chemicals and if semen is not cooled at optimal cooling rates. Hence, it is crucial to know the optimum cooling rates with freezing and thawing protocols for maximum recovery of viable and functional sperm cells for a successful cryo-freezing of ram spermatozoa. Therefore, the current study compiled and compared the research on the impact of different cryopreservation procedures, cooling rates, equilibration time, and thawing protocols on post-thaw ram semen quality.

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Effects of Proportion of Egg Yolk and Preservation Time on Chilled Semen from Indigenous Rams

Cited by 10 publications

References 0 publications

Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

Examining the relationship between progesterone, blood metabolism profile and vaginal smears in lactating Friesian Holstein cows

Cryopreservation Techniques for Ram Sperm

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