Effects of propyphenazone and other non-steroidal anti-inflammatory agents on the synthetic and endogenous androgenic anabolic steroids urinary excretion and/or instrumental detection

Mazzarino, Monica; Braganò, Maria Cristina; Donati, Francesco; Torre, Xavier de la; Botrè, Francesco

doi:10.1016/j.aca.2009.10.022

Cited by 17 publications

(24 citation statements)

References 9 publications

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“…Samples were pre-treated according to a specific procedure accredited according to ISO 17025 and presently followed by the WADA-accredited anti-doping laboratory of Rome [14]. Briefly, to 3 mL of urine, 2 mL of phosphate buffer (1 M, pH 7.4), 50 L of ␤-glucuronidase from E. coli and 50 L of the internal standards mixture (ISTDs: 17␣-methyltestosterone 12 g mL −1 , deuterated T 4 g mL −1 , deuterated E 1 g mL −1 ) were added and the samples were incubated for 1 h at 50 • C. After hydrolysis, to alkalinise the sample 1 mL of carbonate/bicarbonate buffer (0.8 M, pH 9) was added and the liquid/liquid extraction was carried out with 10 mL of tert-butylmethylether for 6 min on a mechanical shaker.…”

Section: Total Fractionmentioning

confidence: 99%

Urine stability and steroid profile: Towards a screening index of urine sample degradation for anti-doping purpose

Mazzarino

Abate

Alocci

et al. 2011

Analytica Chimica Acta

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Section: Total Fractionmentioning

confidence: 99%

Urine stability and steroid profile: Towards a screening index of urine sample degradation for anti-doping purpose

Mazzarino

Abate

Alocci

et al. 2011

Analytica Chimica Acta

Self Cite

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“…In contrast, an observational study of 34 healthy young men reported no association between aspirin use and plasma testosterone 23 . In a study of five sedentary men, treatment with a single 400‐mg dose of propyphenazone was associated with decreased urinary excretion of several testosterone metabolites, but no association was observed for treatment with other NSAIDs 24 . Another study of eight healthy young men reported that aspirin inhibited the increase in plasma testosterone levels typically caused by the administration of human chorionic gonadotrophin 25 .…”

Section: Discussionmentioning

confidence: 89%

Non steroidal anti‐inflammatory drug use and levels of oestrogens and androgens in men

Gates¹,

Araujo²,

Hall³

et al. 2012

Clinical Endocrinology

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Objective Studies suggest that regular use of non-steroidal anti-inflammatory drugs (NSAIDs) may lower estrogen levels in women. However, no large, population-based studies have assessed NSAID/hormone associations in men. Our objective was to examine the association between use of prescription and over-the-counter NSAIDs and levels of estrogens and androgens in men. Design The Boston Area Community Health Survey, an observational survey with initial data collection in 2002–2005. Patients 1,766 men who provided a blood sample and data on recent analgesic use. Measurements Adjusted geometric mean levels of androgens, estrogens, SHBG, LH, and FSH for each category of NSAID use and the percent difference in hormone levels for users vs. non-users. Results There was no significant association between prescription or over-the-counter NSAID use and any hormone examined after adjustment for potential confounders. For example, geometric mean testosterone levels were 13.8, 13.6, and 14.2 nmol/L in non-users, prescription, and over-the-counter NSAID users, respectively; the corresponding levels for estradiol were 80.3, 70.4, and 79.9 pmol/L. In stratified analyses, however, prescription NSAID use was associated with lower testosterone, estradiol, and estrone levels in obese men and lower testosterone and dehydroepiandrosterone sulfate levels in inactive men. Conclusions While overall these data do not provide strong support for an association between NSAID use and hormone levels in men, prescription NSAIDs may decrease levels of certain estrogens and androgens in obese and inactive men.

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“…It was noticed that incomplete derivatization of 13C‐BLD, with more than 99% conversion of androsterone, was frequently associated with higher than average concentrations of major endogenous steroids and/or declaration of certain medications such as non‐steroidal anti‐inflammatory or anti‐histamine drugs. It has been shown earlier that non‐steroidal anti‐inflammatory medications can significantly hamper derivatization of steroids …”

Section: Resultsmentioning

confidence: 99%

Isotopically labeled boldenone as a better marker of derivatization efficiency for improved quality control in anti‐doping analysis

Sobolevsky¹,

Kucherova²,

Ahrens³

2018

Drug Testing and Analysis

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At present, anti‐doping laboratories use androsterone, a major urinary steroid metabolite, to evaluate completeness of the derivatization step. This is typically done by calculating the ratio of mono‐trimethylsilyl (TMS) androsterone to the total mono‐ and di‐TMS androsterone. Certain samples may show an elevated percentage of mono‐TMS androsterone indicating a failed derivatization step. In such cases, the laboratory would have to repeat the analysis or perform other remedial actions to ensure that completeness of derivatization is achieved. We have noticed that a poorly derivatized positive control sample spiked with various target analytes has a disproportionally low abundance of the di‐TMS derivatives of boldenone and 18‐nor‐17β‐hydroxymethyl‐17α‐methylandrosta‐1,4,13‐trien‐3‐one (methandienone long‐term metabolite). A follow‐up investigation confirmed that 1,4‐diene‐3‐one steroids are more likely to fail during the trimethylsilylation step. To better control derivatization efficiency, 13C3‐boldenone (13C‐BLD) was incorporated into our routine procedure as an additional internal standard. Analysis of a large number of urine samples has shown that derivatization of 13C‐BLD could be grossly incomplete even in cases when mono‐TMS androsterone is well below 1%. In other words, one or both of boldenone and the long‐term metabolite of methandienone could remain undetected unless the laboratory has the means to recognize samples where derivatization of 1,4‐diene‐3‐one steroids failed.

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Effects of propyphenazone and other non-steroidal anti-inflammatory agents on the synthetic and endogenous androgenic anabolic steroids urinary excretion and/or instrumental detection

Cited by 17 publications

References 9 publications

Urine stability and steroid profile: Towards a screening index of urine sample degradation for anti-doping purpose

Urine stability and steroid profile: Towards a screening index of urine sample degradation for anti-doping purpose

Non steroidal anti‐inflammatory drug use and levels of oestrogens and androgens in men

Isotopically labeled boldenone as a better marker of derivatization efficiency for improved quality control in anti‐doping analysis

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