Effects of Prostacyclin and Beraprost on Ciliary Motilit&amp;gamma; of Rabbit Airway Epithelium

Tamaoki, Jun; Sakai, Shunsuke; Chiyotani, Atsushi; Takamatsu, Kiyoshi; Tagaya, Etsuko; Konno, Kunihiko

doi:10.1159/000139179

Cited by 9 publications

(4 citation statements)

References 12 publications

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“…For example, some authors have found that erythromycin inhibits the chemotaxis of PMNs, while others have found that it does not. 9,19 These differences may be explained, in part, by technical differences, as some tests were carried out in animals, 19 some used cells harvested from animals treated with the macrolide (ex vivo) 12,14 and some were conducted purely in vitro. 20 In addition, the mechanism by which conventional anti-inflammatory drugs work is complex and not fully understood.…”

Section: Discussionmentioning

confidence: 99%

Comparative anti-inflammatory effects of roxithromycin, azithromycin and clarithromycin

Scaglione

Rossoni²

1998

Journal of Antimicrobial Chemotherapy

120

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There are many published reports on the anti-inflammatory effects of macrolides, some dating back to the introduction of erythromycin. Macrolides have been shown to affect a number of the processes involved in inflammation, including the migration of neutrophils, the oxidative burst in phagocytes and the production of various cytokines, although the precise mechanisms are not clear. These effects have been linked to the ability of macrolides to accumulate in mammalian cells. Roxithromycin, a macrolide with better plasma concentrations and higher tissue concentrations than erythromycin, has been tested in a standard animal model used for evaluating anti-inflammatory drugs. When rats were given a prophylactic dose (20 mg/kg), roxithromycin suppressed the oedema produced by injecting carrageenin into the paw with effects almost equal to that seen with the non-steroidal anti-inflammatory drug nimesulide. Azithromycin and clarithromycin, macrolides with better pharmacokinetics than erythromycin, only showed slight anti-inflammatory effects. These results confirm that roxithromycin has anti-inflammatory properties in vivo and encourage the investigation of its mode of action.

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Section: Discussionmentioning

confidence: 99%

Comparative anti-inflammatory effects of roxithromycin, azithromycin and clarithromycin

Scaglione

Rossoni²

1998

Journal of Antimicrobial Chemotherapy

120

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“…Other investigations have shown that PGE2 acts through cAMP and NO second messengers in rat liver macrophages, 20 and that PGI2 analogs can stimulate ciliary activity via endogenous cAMP production. 21 At higher concentrations, methacholine could stimulate the cyclooxygenase pathway, producing prostaglandins. These endogenous prostaglandins could regulate CBF via stimulation of cAMP production.…”

Section: Resultsmentioning

confidence: 99%

Signal Transduction Pathways in Modulation of Ciliary Beat Frequency by Methacholine

Yang

Schlosser

McCaffrey

1997

Ann Otol Rhinol Laryngol

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The release of endogenous neurotransmitters plays an important role in the airway mucosal defense system. We studied the in vitro effect of methacholine, a ß-methyl ester of acetylcholine, on the ciliary beat frequency (CBF) of human adenoid expiants and its mechanism of action. Tissue expiants were cultured at 35°C and covered with 1.0 mL of culture medium: minimum essential Eagle's medium (MEM) containing L-arginine (1.2 x 10 -3 mol/L). Methacholine was added to the cultured tissue at concentrations of 10~1 0 , 10-8 , and 10 -6 mol/L. The CBF was determined by phase contrast microscopy and microphotometry. Methacholine increased CBF in a dose-dependent manner with a maximum increase of 23.0% ± 1.8% (p < .001). Atropine (10 -6 mol/L) significantly inhibited the ciliostimulatory effects of methacholine (p < .0007). The role of endogenous prostaglandins in methacholine-induced ciliostimulation was determined by treating specimens with a cyclooxygenase inhibitor (diclofenac sodium). Diclofenac (10* 6 mol/L) significantly inhibited the ciliostimulatory effects of methacholine (p < .0007). To determine if nitric oxide (NO) acts as an intermediary in ciliostimulation by methacholine, endogenous NO production was inhibited by treating specimens with an L-arginine analog, N G -nitro-L-arginine methyl ester (L-NAME), prior to addition of methacholine. L-NAME ( 10" 6 mol/L) inhibited the effects of methacholine in L-arginine-free MEM (p < .008), and this inhibition was reversed by L-arginine (10 -3 mol/L). To further examine the actions of NO in methacholine-induced ciliostimulation, a cyclic guanosine 3'5'-monophosphate (cGMP) kinase inhibitor (KT-5823) was used, prior to the addition of methacholine. KT-5823 (lOr 6 mol/L) significantly inhibited the effects of methacholine (p < .0001). Ciliostimulation by methacholine in human upper airway mucosa involves both prostaglandin and NO second messengers and activation of a cGMPdependent kinase.

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“…On the other hand, the increase of cilia motility may be achieved by application of prostacyclin [37], a neuromodulatory peptide -substance P (SP) [38], or methacholine, a non-selective agonist of cholinergic (muscarinic) receptors [39]. Also, it has been proven that cilia beats frequency may increase following the activation of adrenergic and purinergic receptors [15,40].…”

Section: "Modifiers" Of Cilia Movementmentioning

confidence: 99%

Chronic inflammation in the respiratory tract and ciliary dyskinesia

Grzela¹,

Zagórska²,

Jankowska‐Steifer³

et al. 2013

cejoi

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Effects of Prostacyclin and Beraprost on Ciliary Motilitγ of Rabbit Airway Epithelium

Cited by 9 publications

References 12 publications

Comparative anti-inflammatory effects of roxithromycin, azithromycin and clarithromycin

Comparative anti-inflammatory effects of roxithromycin, azithromycin and clarithromycin

Signal Transduction Pathways in Modulation of Ciliary Beat Frequency by Methacholine

Chronic inflammation in the respiratory tract and ciliary dyskinesia

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