Effects of protease inhibitors on dentin erosion: an in situ study

Yang, Hui; Lin, Xue‐Jun; Liu, Qiong; Yu, Hao

doi:10.1007/s00784-022-04657-w

Cited by 6 publications

(15 citation statements)

References 35 publications

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“… 8 However, DOM can be degraded by collagenases (e.g., matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs)), leading to increased mineral loss in demineralized dentin and further accelerating the progression of dentin erosion. 8 - 10 In previous studies, the application of both MMP 5 , 11 and CC inhibitors 5 significantly reduced erosive dentin loss (EDL) by approximately 21–42%.…”

Section: Introductionmentioning

confidence: 94%

“…3 , 4 During the progression of dentin erosion, minerals are released from the peritubular and intertubular dentin. 5 Thereafter, a layer of a fully demineralized organic matrix (DOM) is formed in the superficial dentin, followed by a partially demineralized zone and by the intact inner dentin. 4 , 5 DOM is regarded as a diffusion barrier against erosive acids and active ingredients released by demineralization, as well as a form of resistance to abrasive forces.…”

Section: Introductionmentioning

confidence: 99%

“… 5 Thereafter, a layer of a fully demineralized organic matrix (DOM) is formed in the superficial dentin, followed by a partially demineralized zone and by the intact inner dentin. 4 , 5 DOM is regarded as a diffusion barrier against erosive acids and active ingredients released by demineralization, as well as a form of resistance to abrasive forces. 6 , 7 Furthermore, maintaining DOM is important for dentin remineralization, especially in the presence of calcium and phosphate together with fluoride.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, MMPs and CCs may synergistically contribute to the progression of dentin erosion. 5 , 13 , 15 In our previous study, 1,10-phenanthroline (an MMP inhibitor), E-64 (a CC inhibitor), and a combination of the two inhibitors were applied before erosion, aiming to reduce EDL. 5 In that study, EDL was significantly reduced and a thicker DOM was preserved compared to that of the control group.…”

Section: Introductionmentioning

confidence: 99%

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Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

et al. 2023

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Objectives To evaluate the effects of matrix metalloproteinase (MMP) and cathepsin K (catK) inhibitors on resistance to dentin erosion. Methodology A total of 96 dentin specimens (3×3×2 mm) were prepared and randomly assigned into four groups (n=24): deionized water (DW); 1 µM odanacatib (ODN, catK inhibitor); 1 mM 1,10-phenanthroline (PHEN, MMP inhibitor); and 1 µM odanacatib + 1 mM 1,10-phenanthroline (COM). Each group was further divided into two subgroups for the application of treatment solutions before (PRE) and after erosive challenges (POST). All specimens were subjected to four daily erosive challenges for 5 d. For each erosive challenge, the specimens in subgroup PRE were immersed in the respective solutions before cola drinks, while the specimens in subgroup POST were immersed in the respective solutions after cola drinks (the immersion duration was 5 min in both cases). All specimens were stored in artificial saliva at 37°C between erosive challenges. The erosive dentin loss (EDL) was measured by profilometry. The residual demineralized organic matrix (DOM) of specimens was removed using type VII collagenase and evaluated by profilometry. Both the EDL and thickness of the residual DOM were statistically analyzed by two-way analysis of variance (ANOVA) and Bonferroni’s test (α=0.05). The surface topography and transverse sections of the specimens were observed using SEM. MMPs and catK were immunolabeled in the eroded dentin and in situ zymography was performed to evaluate the enzyme activity. Results Significantly lower EDL was found in the groups ODN, PHEN, and COM than in the control group (all p<0.05), while no significant difference in EDL was found among the groups ODN, PHEN, and COM (all p>0.05). The application sequence showed no significant effect on the EDL of the tested groups (p=0.310). A significantly thicker DOM was observed in the group ODN than in the control group regardless of the application sequence (both p<0.05). The treatment with ODN, PHEN, and COM inhibited the gelatinolytic activity by approximately 46.32%, 58.6%, and 74.56%, respectively. Conclusions The inhibition of endogenous dentinal MMPs and catK increases the acid resistance of human dentin but without an apparent synergistic effect. The inhibition of MMPs and catK is equally effective either before or after the acid challenge.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

et al. 2023

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“…Of late, other compounds have been presented, such as 2% doxycycline (56), 1,10-phenanthroline (57), captopril (58), ionreleasing filler (59), and Emblica officinalis (Indian gooseberry or amla) as an acid etchant and MMP inhibitor. Amla juice has a similar etching effect as a self-etch adhesive in SEM and 100% amla extract has been found to inhibit MMP-9 by gelatin zymography (60) and a novel mussel-inspired monomer [N- (3,4-dihydroxyphenethyl) methacrylamide] (61) with the aim of the preservation of the bonding between the restorative material and the tooth wall.…”

Section: Matrix Metalloproteinase Inhibitors and Collagen Cross-linki...mentioning

confidence: 99%

Insight into the development of versatile dentin bonding agents to increase the durability of the bonding interface

Porto

Lôbo²,

Rodrigues³

et al. 2023

Front. Dent. Med

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Despite the huge improvements made in adhesive technology over the past 50 years, there are still some unresolved issues regarding the durability of the adhesive interface. A complete sealing of the interface between the resin and the dentin substrate remains difficult to achieve, and it is doubtful whether an optimal interdiffusion of the adhesive system within the demineralized collagen framework can be produced in a complete and homogeneous way. In fact, it is suggested that hydrolytic degradation, combined with the action of dentin matrix enzymes, destabilizes the tooth-adhesive bond and disrupts the unprotected collagen fibrils. While a sufficient resin–dentin adhesion is usually achieved immediately, bonding efficiency declines over time. Thus, here, a review will be carried out through a bibliographic survey of scientific articles published in the last few years to present strategies that have been proposed to improve and/or develop new adhesive systems that can help prevent degradation at the adhesive interface. It will specially focus on new clinical techniques or new materials with characteristics that contribute to increasing the durability of adhesive restorations and avoiding the recurrent replacement restorative cycle and the consequent increase in damage to the tooth.

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The activity, distribution, and colocalization of cathepsin K and matrix metalloproteases in intact and eroded dentin

Lin,

Tong,

Chen

et al. 2023

Clin Oral Invest

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Effects of protease inhibitors on dentin erosion: an in situ study

Cited by 6 publications

References 35 publications

Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion?

Insight into the development of versatile dentin bonding agents to increase the durability of the bonding interface

The activity, distribution, and colocalization of cathepsin K and matrix metalloproteases in intact and eroded dentin

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