Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Sant’Ana, Débora de Mello Gonçales; Molinari, Sônia Lucy; Miranda-Neto, Marcílio Hubner de

doi:10.1590/s0004-282x2001000400003

Cited by 17 publications

(28 citation statements)

References 12 publications

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“…This decrease can probably be explained by the smaller body weight of the diabetic animals, which was accompanied, although not proportionately, by a diminishment of the internal organs: in percentual terms, the diabetics had a body weight loss of 16.10% relative to their initial weight, while the colon showed a weight decrease of 13.45% as compared to that of the controls. Smaller intestinal segments were frequently observed in our laboratory, specially in animals losing weight due to protein desnutrition 22,23 . Insulin deficiency is also an explanation for the reduced dimensions observed here, once insulin is an anabolic hormone and, as such, a promoter of cellular development.…”

Section: Discussionmentioning

confidence: 91%

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Furlan

Molinari

Neto

2002

Arq. Neuro-Psiquiatr.

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-The effects of acute diabetes on the density and size of the myenteric neurons of the proximal colon of adult rats were investigated. The injection of streptozotocin was followed by a period of observation of seven days, during which the diabetic animals showed weight loss, excessive food and water intake, large urinary debt and hyperglicemia. The whole-mounts from the proximal colon were stained with the techniques of Giemsa and of the NADH-diaphorase, and the employment of these techniques made it possible to verify a decrease on the neuronal density and on the cell body size of the myenteric neurons in the colon of the diabetic rats. These observations were discussed in terms of the pathophysiology of the diabetes and the experimental protocol.KEY WORDS: acute diabetes, myenteric neurons, proximal colon.Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos Efeitos morfoquantitativos do diabetes agudo sobre os neurônios mioentéricos do colo proximal de ratos adultos RESUMO -Foram investigados os efeitos do diabetes agudo sobre a densidade e o tamanho dos neurônios mioentéricos do colo proximal de ratos adultos. À injeção de estreptozootocina seguiu-se um período de observação de sete dias, durante os quais os animais diabéticos apresentaram perda de peso, ingestão excessiva de alimento e água, grande débito urinário e hiperglicemia. Os preparados de membrana do colo proximal foram corados pelas técnicas de Giemsa e da NADH-diaforase. A aplicação dessas técnicas permitiu constatar uma redução da densidade neuronal e do tamanho do corpo celular dos neurônios mioentéricos no colo dos ratos diabéticos. Essas observações foram discutidas em termos da patofisiologia do diabetes e do protocolo experimental. The research on the changes induced by experimental diabetes on the several tissues and organs of laboratory animals is quite large. Among those systems under investigation are the gastrointestinal tract and its intrinsic enteric nervous system, the neuronal network responsible for the control of the activities of the bowel. It is reported, for instance, that the myenteric neurons of the stomach, duodenum and cecum are numerically reduced in diabetes 1-3 , and that specific neurochemical groups show response patterns to diabetes which depend on the intestinal segment and the duration of the diabetic state 4-7 . These neuronal changes, as well as those associated to the autonomic innervation of the gut 8-10 , stand among the responsible by the clinical gastrointestinal symptoms of diabetes [11][12][13] . PALAVRAS-CHAVERecently, we described an increase in the NADHdiaphorase positive myenteric neuronal population in the duo...

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Section: Discussionmentioning

confidence: 91%

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Furlan

Molinari

Neto

2002

Arq. Neuro-Psiquiatr.

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“…The diet of the experimental group was obtained reducing the protein levels of the NUVILAB chow through the addition of corn starch and mixture of mineral salts, but without vitamin supplementation 14,15 . This procedure was based on models described elsewhere 1,5,6,12,21,22 .…”

Section: Methodsmentioning

confidence: 99%

“…In the same segment of adult rats subjected to protein and vitamin deficiency, it was observed a greater reduction in the total number of myenteric neurons 14,15 .…”

mentioning

confidence: 87%

“…In what concerns the effects of undernourished on the myenteric plexus, the hypoproteic diet leads to a greater concentration of neurons 5,10-12 and diminishment of their numbers [13][14][15] . Nevertheless, most of the studies on the effects of malnutrition on the enteric neurons assess the small intestine 5,8,10,13,16 , the number of works dealing with the large intestine being reduced 1,11,12,14,15,17 .…”

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confidence: 99%

“…Nevertheless, most of the studies on the effects of malnutrition on the enteric neurons assess the small intestine 5,8,10,13,16 , the number of works dealing with the large intestine being reduced 1,11,12,14,15,17 . Studies in the ascending colon of rats subjected to protein deficiency during gestation and lactation demonstrated increased neuronal density and incidence of large neurons 11 , while animals subjected to apoproteic diet during gestation and lactation had changes in the myenteric ganglia shape, reduction in the area of the neuronal profile and greater density of the neurons in the total colon 17 .…”

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confidence: 99%

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Effect of protein and vitamin B deficiency on the morpho-quantitative aspects of the myenteric plexus of the descending colon of adult rats

Araújo

Sant’Ana

Molinari

et al. 2003

Arq. Neuro-Psiquiatr.

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We carried out this work with the purpose of studying the effects of protein and vitamin B deficiency on the morphologic and quantitative aspects of the myenteric plexus of the descending colon of adult Rattus norvegicus. Twenty-eight rats were divided in two groups, one of them receiving chow with 22% protein level (control) and the other fed with chow having 8% protein level without vitamin B supplementation, during 120 days. Whole-mounts of the descending colon were prepared and stained with Giemsa, NADH-diaphorase and NADPH-diaphorase. The undernourished rats had a body weight 11.84% less than the control group. Relative to the controls, the experimental group had a colonic area 48% smaller, 51.9% less Giemsa-stained neurons, 28.3% less NADH-diaphorase positive neurons and 24.2% less NADPH-diaphorase positive neurons.

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The myenteric plexus of the rat colon after fecal stream diversion: a morpho-quantitative study

Fontes

Froes

Omar

et al. 2004

Autonomic Neuroscience

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Effects of protein and vitamin B deficiency on blood parameters and myenteric neurons of the colon of rats

Cited by 17 publications

References 12 publications

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Morphoquantitative effects of acute diabetes on the myenteric neurons of the proximal colon of adult rats

Effect of protein and vitamin B deficiency on the morpho-quantitative aspects of the myenteric plexus of the descending colon of adult rats

The myenteric plexus of the rat colon after fecal stream diversion: a morpho-quantitative study

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