Effects of protein deficiency induced by raw soy with and without sucrose on dentine formation and dentinal caries in young rats

Huumonen, Sisko; Larmas, Markku

doi:10.1016/j.archoralbio.2004.10.011

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…This is consistent with previously published results examining molars from rat pups whose mothers were submitted to a protein deficient diet during gestation and lactation [10]. Importantly, reduced tooth size in undernourished animals is thought to result from a decrease in the volume of the dentin rather than a reduction in the thickness of the enamel [10, 22]. …”

Section: Discussionsupporting

confidence: 91%

“…Fluoride deficiency in rats maintained on a low-protein diet increased enamel deposition by 48% and decreased dentin by 28% [21]. Moreover, reduced dentin formation was observed among rats submitted to a protein-deficient diet [22]. Recently, the effects of undernutrition on enamel defects in socio-economically underprivileged communities have begun to be appreciated [23].…”

Section: Introductionmentioning

confidence: 99%

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Structural and Ultra-Structural Features of the First Mandibular Molars of Young Rats Submitted to Pre and Postnatal Protein Deficiencies

Gonçalves

Boldrini²,

Capote³

et al. 2009

Open Dent J

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The effects of protein malnutrition, both in utero and prior to weaning, on formation of the first mandibular molars were evaluated by phase-contrast and electron microscopy in rats. The nourished group (GI) received a diet that included 20% casein, while the malnourished group (GII) received 5% casein. The first mandibular molars from GII exhibited low density of cells and odontoblasts, which lacked regular organization compared with molars from GI. In addition, a difference in collagen type was observed between the groups, with a prevalence of Type III collagen fibers detected in the dentin, periodontal ligament, and alveolar bone of GII, and a prevalence of Type I collagen fibers in GI. Finally, examination of surface area in molar sagittal sections indicated 30% less dentin in GII, compared with GI. Our results suggest that structural and ultra-structural features of the dentin-pulp complex and periodontal components of rat molars are affected by protein deficiency.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Structural and Ultra-Structural Features of the First Mandibular Molars of Young Rats Submitted to Pre and Postnatal Protein Deficiencies

Gonçalves

Boldrini²,

Capote³

et al. 2009

Open Dent J

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show abstract

“…The results of this experiment also corroborate a research done by Huumonen and Larmas [16] who investigated the effects of protein deficiency and sucrose in the formation and mineralization of dentin in Wistar rat molars. Tetracycline was injected at the outset to mark the dentin formed up to that moment, and the average of 3 measurements made in the cleft of the primary molars was calculated.…”

Section: Discussionsupporting

confidence: 80%

“…Some studies have identified that undernourished rats, before and after birth, have marked reduction in teeth size, which has been attributed to a decrease in volume of dentin but not to the thickness of the enamel [12,16]. This may be related to changes in the secretion of collagen [11].…”

Section: Introductionmentioning

confidence: 99%

Impact of prenatal protein-calorie malnutrition on the odontogenesis of wistar rats

Rocha

Baldani

Lopes

2013

BDS

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Impact of prenatal protein-calorie malnutrition on the odontogenesis of wistar rats RESUMO Objetivo: Este trabalho teve por objetivo determinar alterações na odontogênese decorrentes da desnutrição protéico-calórica pré e pós-natal. Foram selecionados 12 ratos Wistar adultos, 8 fêmeas e 4 machos. As fêmeas foram divididas em dois grupos, sendo que um deles recebeu dieta normoprotéica (G1) e o outro recebeu dieta hipoprotéica (G2). Material e Métodos: Após o nascimento das ninhadas, foram separados aleatoriamente 24 filhotes de cada grupo. Os animais foram sacrificados, 12 aos 5 dias de vida e 12 aos 8, e suas mandíbulas submetidas à preparação histológica para a obtenção de cortes dos germes dentários. Foram selecionadas 48 lâminas que apresentavam os germes adequadamente cortados (24 de cada grupo), as quais foram analisadas em microscópio e medidas por um examinador calibrado utilizando-se o software Image Pro Plus for Windows, versão 6.0. As diferenças entre as médias foram verificadas mediante o teste não paramétrico de Mann Whitney. Resultados: Os resultados obtidos demonstram que, aos 5 dias, as diferenças de espessura de esmalte e dentina foram estatisticamente significantes entre G1 (84,08 ± 28,9 e 141,51 ± 33,2; p = 0,026) e G2 (47,26 ± 43,8 e 91,19 ± 54,7; p = 0,006). Aos 8 dias de vida não foram identificadas diferenças significativas entre os grupos. Conclusão: Os resultados demonstraram indícios de impacto da desnutrição sobre a espessura dos tecidos dentários. Sugere-se o aprofundamento desta linha de investigação com delineamentos mais complexos.Impacto da desnutrição protéico-calórica pré-natal sobre a odontogênese de ratos wistar ABSTRACTObjective: This study aimed to determine changes in odontogenesis arising from prenatal and postnatal protein-calorie malnutrition. Material and Methods: Twelve adult Wistar rats were selected; 8 females and 4 males. The females were divided into two groups, one of which received a normoproteic diet (NG) and the other received a hypoproteic diet (HG). After the birth of the litters, 24 pups were randomly separated from each group. The animals were sacrificed, 12 at five-days old and 12 at eight-days old, and their jaws were subjected to histological preparation to obtain cuts of tooth germs. Forty-eight slides were selected that presented the germs properly cut (24 from each group), which were analyzed by microscopy and measured by a calibrated examiner. The differences between means were verified by the nonparametric Mann-Whitney test. Results: The results showed that at 5 days the differences in thickness of enamel and dentin were statistically significant between NG (84.08 ± 28.9 and 141.51 ± 33.2; p = 0.026) and HG (47.26 ± 43.8 and 91.19 ± 54.7; p = 0.006). At 8 days of life there were no significant differences between the groups. Conclusion: The results showed evidence of the impact of malnutrition on the thickness of dental tissues. It is suggested that further work should be carried out in this line of research with more complex designs.

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Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats

Kołodziej

Maciejczyk

Niklińska

et al. 2017

Archives of Oral Biology

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Effects of protein deficiency induced by raw soy with and without sucrose on dentine formation and dentinal caries in young rats

Cited by 6 publications

References 20 publications

Structural and Ultra-Structural Features of the First Mandibular Molars of Young Rats Submitted to Pre and Postnatal Protein Deficiencies

Structural and Ultra-Structural Features of the First Mandibular Molars of Young Rats Submitted to Pre and Postnatal Protein Deficiencies

Impact of prenatal protein-calorie malnutrition on the odontogenesis of wistar rats

Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats

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