Effects of protein, mucin, and human tears on adherence of Pseudomonas aeruginosa to hydrophilic contact lenses

Miller, Michael J.; Wilson, Louis A.; Ahearn, Donald G.

doi:10.1128/jcm.26.3.513-517.1988

Cited by 59 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the opposite was true for polymacon lenses, with higher HSA deposition being associated with lower bacterial adherence. Other studies have confirmed that some tear-coated contact lens materials enhance adhesion of P. aeruginosa, but high individual variation is always reported [26,92].…”

Section: Albumin Adsorption At Biomaterials Interfacesmentioning

confidence: 97%

Albumin adsorption to contact lens materials: A review

Luensmann

Jones

2008

Contact Lens and Anterior Eye

View full text Add to dashboard Cite

During contact lens wear, tear film components such as lipids, mucins and proteins tend to deposit on and within the lens material and may cause discomfort, reduced vision and inflammatory reactions. The tear film protein that has attracted most interest when studying contact lens deposition is the small (14 kDa), positively charged protein lysozyme. Albumin, which is a much larger protein (66 kDa) with an overall net negative charge is also of interest, and shows very different adsorption patterns to lysozyme. The concentration of albumin in the tear film is relatively low compared to the concentration in blood serum, but this value increases markedly under various conditions, including when the eye is closed, during contact lens wear and in various dry eye states. Gaining an understanding of the manner in which albumin deposits on biomaterials is of importance for contact lens wear, as well as for other medical applications where HEMA-based materials are used for implants, artificial blood vessels or drug delivery devices. This review paper summarizes the impact of individual material compositions, water content, hydrophobicity and electrostatic attraction on the adsorption behavior of the protein albumin.

show abstract

Section: Albumin Adsorption At Biomaterials Interfacesmentioning

confidence: 97%

Albumin adsorption to contact lens materials: A review

Luensmann

Jones

2008

Contact Lens and Anterior Eye

View full text Add to dashboard Cite

show abstract

“…Surfaces in the oral cavity are covered by a salivary pellicle which serves diverse functions, among which are tissue lubrication and protection, and clearance or adherence of oral flora (34,55). Salivary glycoproteins are thought to play an important role in modulation of microbial colonization of many biological surfaces (33,39,50,53). Components in whole saliva including mucins have been reported to adsorb to C. albicans in significantly greater quantities than other proteins such as albumin, lysozyme, fibrinogen, or whole serum (43).…”

mentioning

confidence: 99%

Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate

et al. 1993

View full text Add to dashboard Cite

The purpose of this study was to identify components of saliva that interact with Candida albicans in solution and that may modulate adhesion to dental acrylic (polymethylmethacrylate [PMMAJ) surfaces. Saliva-derived pellicles extracted from C. albicans blastoconidia and hyphal-form cells mixed with fresh human submandibular-sublingual saliva (HSMSL) contained predominantly high-and low-molecular-weight mucins (MG1 and MG2, respectively). In contrast, few components from fresh human parotid saliva were adsorbed to yeast cells. Coating PMMA beads with HSMSL significantly enhanced (10-fold) adhesion of both growth forms of C. albicans compared with human parotid saliva (2-fold), suggesting a role for mucins in adhesion. HSMSLenhanced adhesion was completely abolished by preadsorbing HSMSL with either blastoconidia or hyphalform cells prior to coating PMMA. However, coating PMMA with purified salivary mucins or the addition of mucin to preadsorbed saliva did not enhance or restore adhesion to levels found with fresh HSMSL. Adhesion assays employing guanidine-treated fresh HSMSL showed a complete lack of Candida binding, suggesting that subjecting HSMSL to dissociating conditions may alter a property of salivary mucins crucial for C. albicans adhesion. Protease and glycosidase treatment of yeast cel[s significantly reduced adhesion to HSMSL-coated PMMA. In addition, preincubation of C. albicans with mannose and galactose inhibited adhesion to HSMSL-coated PMMA. These results suggest that mucins may play a role in C. albicans adhesion to saliva-coated PMMA and that a glycoprotein on the yeast surface may be involved in these events.

show abstract

“…Further analysis is necessary to determine if this difference in spectra directly affects bacterial adhesion and biofilm formation. Factors such as water content of lens material and surface hydrophobicity of bacteria are often exploited to limit bacterial contamination of contact lenses (13,15,21,22). Multiple studies have shown that there is an inverse relationship between bacterial adhesion and water content (15,(17)(18)(19)(21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

“…The materials employed to fabricate a contact lens influences the incidence of contact lens‐associated microbial keratitis . The type of polymers used in the manufacturing of contact lenses can enhance or interfere with bacterial adhesion.…”

Section: Discussionmentioning

confidence: 99%

Identification of Microorganisms Isolated From Counterfeit and Unapproved Decorative Contact Lenses

Land

Penno

Brzezinski

2017

Journal of Forensic Sciences

View full text Add to dashboard Cite

All contact lenses (corrective/noncorrective) are considered Class II or Class III medical devices under the Federal Food, Drug, and Cosmetic Act, which also states that contact lenses can only be obtained with a prescription. The Forensic Chemistry Center of the US Food & Drug Administration has examined over 300 decorative, noncorrective contact lenses obtained without a prescription. Our observations indicate that 60% of the counterfeit lenses and 27% of the unapproved lenses examined were positive for microbial contamination. Twenty-nine different brands of noncorrective contact lenses were examined, and 48% of them had at least one sample positive for microbial contamination. Each microorganism was further identified using DNA sequencing. Contaminated contact lenses are associated with numerous health risks, including ocular infections and conjunctivitis leading to permanent visual impairment or blindness. These results support the contention that acquiring contact lenses without a prescription is a considerable threat to consumer health and safety.

show abstract

Effects of protein, mucin, and human tears on adherence of Pseudomonas aeruginosa to hydrophilic contact lenses

Cited by 59 publications

References 26 publications

Albumin adsorption to contact lens materials: A review

Albumin adsorption to contact lens materials: A review

Human submandibular-sublingual saliva promotes adhesion of Candida albicans to polymethylmethacrylate

Identification of Microorganisms Isolated From Counterfeit and Unapproved Decorative Contact Lenses

Contact Info

Product

Resources

About