Effects of protein supplements on fertility and assessment of the fertility genes (GDF9andBMP15) in indigenous sheep of Bangladesh

Abstract

The study was conducted to investigate the effect of different levels of protein supplements on fertility and assess the fertility gene (BMP15 and GDP9) in sheep of Bangladesh. Thirty six ewes fed two protein level (Treatment 1, T1 and treatment 2, T2) and no protein supplement ration (control, T0). Polymerase chain reactions (PCR) were performed from blood cell DNA following standard protocols. PCR products were sequenced using Sanger sequencing and MEGA6 software were used for molecular analysis. The conception rate of ewes was significantly higher (P < 0.05) in the treated group than the control. Gestation period and lambing interval were significantly higher (worse) in control (154.00 ± 0.57 and 186.00 ± 1.24 days) than treated groups. Birth weight of lamb ranged from 1.07 to 1.65 kg and higher birth weight was found in T2 than other groups. Sequence alignment of BMP15 and GDF9 gene in indigenous sheep showed 100% homology with other sheep breed. As no mutation was observed and apparently these genes have no effect on ewe fertility. However, nutritional supplements had a positive impact on reproductive performance. The phylogenetic inferences have close relations of Bangladeshi sheep with other breed elsewhere.

POLYMORPHISMS IN BMPR1B AND INHβA GENES ARE ASSOCIATED WITH LITTER SIZE IN INDIGENOUS SHEEP OF BANGLADESH

“…However, sequence alignment showed four other selected mutations in GDF9 [c.260G>A (FecG1) and c.1184C>T (FecG8)] and BMP15 [c.69C>T (FecX G and c.718C>T (FecX HIL )] gene fragments were monomorphic in the studied populations that limits for further PCR-RFLP genotyping. Similar to the present findings, Hossain et al (2020) reported the absence of polymorphisms in BMP15 and GDF9 genes of indigenous sheep of Bangladesh. This finding partially agrees with the results of Roy et al (2011) who reported all considered loci of BMP15 and GDF9 genes were monomorphic except two (G1 and G4) of GDF9 were found to be polymorphic in Indian Bonpala sheep.…”

Effect of Dry Season Supplementary Feeding and Parity on Performance of Desert Sheep in North Kordofan State, Sudan

“…Parity order affected significantly the reproductive parameters, old ewes positively showed higher conception and lambing rate, this finding was similar to the other researcher results [10,11,22,23,43,44] and [34] demonstrated that they increasing the age of ewes could increase the pregnancy rate and multiple births, also [26] reported that ewe age significantly affected both the pregnancy and lambing rates, primiparous ewes had a higher lambing rate (72.0%) than that (44.2%) of multiparous ewes, and he concluded that old aged ewes had a less conception rate than 1 to 2 years aged ewes. Also [43] reported that the fertility rate was lower in 1.5-year-old ewes than in older ewes.…”

“…Also agreed with [58] he demonstrated that increase in reproductive rate in supplemented ewes was due to increases in both conception rate and fecundity, with a higher proportion of pregnant ewes, and a higher proportion of multiple pregnancies, in the supplemented groups. Contrast to these results [34] reported that the number of fetuses per ewe pregnant was not influenced by level of nutrition. Results in this study showed that ewes depend on natural grazing alone scored lower litter size, similar result obtained by [59] who stated explained lower litter size in terms of the scarcity and low quality of the forage in the tropical rangelands, which are subject to uncertain seasonal rainfall patterns, and where supplemental feed is also scarce and economically unfeasible.…”

“…[35] stated that nutrient deficiency potentially acts on the reproductive process and affects estrus behavior and ovulation rate. [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36] reported differences in nutrition probably account for most of the variation in reproductive performance. Also [31] stated that the strategic supplementary feeding of ewes increased lambing rates of ewes, agreed with [37] that poor nutrition leads to reduce conception and lambing rates.…”

Morphometric traits with reproduction and production performances of indigenous and exotic sheep breeds of Bangladesh

“…Table 3 shows the variations of the post-partum intervals of Bangladeshi indigenous and exotic cross breeds sheep under reviewed works. Hossain et al, (2020) AFS-Age at first service, GL-Gestation length, AFL-Age at first lambing, LI-Lambing interval, PPI-Post-partum interval,…”