Effects of PRP and LyPRP on osteogenic differentiation of MSCs

Abstract

Platelet-rich plasma (PRP) is rich in a variety of growth factors and plays an important role in the proliferation and differentiation of mesenchymal stem cells (MSCs). It has been reported that the preparation of freeze-dried platelets (lyophilized platelets [LyPRP]) from platelets could be an effective strategy to preserve the bioactivity of platelets for a long time. In this study, the osteogenic induction effects of PRP and LyPRP on MSCs were evaluated. The rabbit arterial blood was drawing to preparation of PRP by secondary centrifugation. Whole blood was prepared by lyophilization buffer to prepare LyPRP, which were activated by chloride and their surface morphology was observed. It was observed using a scanning electron microscope that platelets were evenly distributed on the surface of PRP and LyPRP. Growth factors were slowly released from PRP and LyPRP during the first 7 days and detected by the enzyme-linked immunosorbent assay kit. Cell proliferation assays and fluoresceindiacetate/propidium iodide (FDA/PI) staining demonstrated that PRP and LyPRP could promote cell proliferation. PRP and LyPRP were also shown to promote osteogenic differentiation of MSCs in vitro by osteogenesis characteristic staining and qPCR quantitative detection of osteogenic related gene expression. Both PRP and LyPRP could promote the proliferation and osteogenic differentiation of MSCs effectively. Moreover, PRP exhibited a better osteogenic induction effect on MSC than LyPRP. K E Y W O R D S BMSCs, LyPRP, osteogenic induction, PRP

Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression

“…However, IPFP-MSCs had less expression of chondrogenic markers in PRP group. We presumed that PRP could promote the osteogenic differentiation of MSCs as some studies have shown [ 89 , 90 ].…”

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

“…Moreover, the equivalence between fresh-frozen PRP and FD-PRP was shown in terms of PDGF-BB, TGF-β and VEGF concentrations and stimulation of bone formation in immunocompromised mice [64]. However, in an apparent contradiction, rabbit BMSCs differentiation and proliferation was better in CaCl 2 -activated fresh PRP compared with FD-PRP [65].…”

Electrospinning With Lyophilized Platelet-Rich Fibrin Has the Potential to Enhance the Proliferation and Osteogenesis of MC3T3-E1 Cells

“…Electrospinning nanofibers with sustained release of growth factors have the great potential to create a beneficial microenvironment for bone tissue formation, including promoting migration and differentiation of osteoblasts by angiogenic, inflammatory, and bone formation enhancement (Sui et al, 2007;Vo et al, 2012). It is interesting that the cytokines from PRF also have similar biological functions for tissue regenerative medicine, such as anti-inflammation effects, promoting osteogenic differentiation and vascularization (Pietramaggiori et al, 2006;You et al, 2019;Nasirzade et al, 2020;Xie et al, 2020;Zhang et al, 2020). In present study, we investigated the effect of extract of PVA/SA/PRF nanofibers on proliferation and osteogenesis of osteogenic precursor cells (MEC3T3-E1 cells) in vitro.…”