Effects of pulpotomy using mineral trioxide aggregate on prostaglandin transporter and receptors in rat molars

Abstract

Mineral trioxide aggregate (MTA) is a commonly used dental pulp-capping material with known effects in promoting reparative dentinogenesis. However, the mechanism by which MTA induces dentine repair remains unclear. The aim of the present study was to investigate the role of prostaglandin E2 (PGE2) in dentine repair by examining the localisation and mRNA expression levels of its transporter (Pgt) and two of its receptors (Ep2 and Ep4) in a rat model of pulpotomy with MTA capping. Ep2 expression was detected in odontoblasts, endothelial cells, and nerve fibres in normal and pulpotomised tissues, whereas Pgt and Ep4 were immunolocalised only in the odontoblasts. Moreover, mRNA expression of Slco2a1 (encoding Pgt), Ptger2 (encoding Ep2), and Ptger4 (encoding Ep4) was significantly upregulated in pulpotomised dental pulp and trigeminal ganglia after MTA capping. Our results provide insights into the functions of PGE2 via Pgt and Ep receptors in the healing dentine/pulp complex and may be helpful in developing new therapeutic targets for dental disease.

Immunohistochemistry and gene expression of GLUT1, RUNX2 and MTOR in reparative dentinogenesis

“…Overall, the wound healing process in the present study was similar to that described in our previous report (Ohkura et al, ). Specifically, the primary process of reparative dentinogenesis after pulpotomy with MTA capping followed moderate inflammatory and necrotic changes at the exposed site (Figures b, b and b), and a dentine bridge was formed in all samples at 14 days after treatment (Figures f, f and f).…”

“…Overall, the wound healing process in the present study was similar to that described in our previous report (Ohkura et al, 2017).…”

“…For double immunofluorescence staining, the pretreated sections were washed with PBS and then incubated for 24 hr at 4°C with a cocktail of mouse anti‐rat endothelial cell antigen 1 (RECA1; 1:50, BioRad, Hercules, CA), anti‐S100 β‐subunit (1:2000, Sigma‐Aldrich, St. Louis, MO) and anti‐Nestin (1:100; Millipore, Darmstadt, Germany) antibodies used to identify endothelial cells (Ohkura et al, ), Schwann cells (Ohkura et al, ) and odontoblasts (About, Laurent‐Maquin, Lendahl, & Mitsiadis, ), respectively. Nestin, which is a useful marker for odontoblast differentiation during odontogenesis (Quispe‐Salcedo, Ida‐Yonemochi, Nakatomi, & Ohshima, ), is also expressed in the newly differentiated odontoblast‐like cells following pulp capping with MTA (Kuratate et al, ; Ohkura et al, ). After rinsing with PBS, the sections were further incubated for 1 hr with a mixture of goat anti‐rabbit IgG‐conjugated Alexa Fluor 488 (1:200, Thermo Fisher Scientific, Waltham, MA) and goat anti‐mouse IgG conjugated to Alexa Fluor 546 (1:200, Thermo Fisher Scientific).…”

“…2000, Sigma-Aldrich, St. Louis, MO) and anti-Nestin (1:100; Millipore, Darmstadt, Germany) antibodies used to identify endothelial cells(Ohkura et al, 2017), Schwann cells(Ohkura et al, 2017) and odontoblasts(About, Laurent-Maquin, Lendahl, & Mitsiadis, 2000), respectively. Nestin, which is a useful marker forodontoblast differentiation during odontogenesis (Quispe-Salcedo, Ida-Yonemochi, Nakatomi, & Ohshima, 2012), is also expressed in the newly differentiated odontoblast-like cells following pulp capping with MTA (Kuratate et al, 2008; Ohkura et al, 2017).…”

Clinical, Radiographic and Histological Evaluation of Primary Teeth Pulpotomy Using MTA And Ferric Sulfate

“…Pulpotomy is a common therapy for asymptomatic cariously exposed pulp with no involvement of the radicular portion, thus avoiding the premature loss of the tooth (1). Successful pulpotomy procedure depends not only on the correct diagnosis of the inflamed dental pulp, but also on the selection of an effective and biocompatible medicament (1,3). The ideal capping material should be bactericidal and harmless to cells and surrounding structures, promote healing of the pulp tissue and not interfere with the physiologic root resorption (4)(5)(6).…”