Effects of Pulsed Electromagnetic Fields on Bone Mass and Wnt/β-Catenin Signaling Pathway in Ovariectomized Rats

Zhou, Jun; He, Hongchen; Yang, Lin; Chen, Shiju; Guo, Hua; Xia, Lu; Liu, Huifang; Qin, Youwen; Liu, Chuan; Wei, Xiaofei; Zhou, Yujing; He, Chengqi

doi:10.1016/j.arcmed.2012.06.002

Cited by 76 publications

(82 citation statements)

References 44 publications

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“…As our previous description [7], blood was collected from all of the rats and centrifuged at 2,000g to extract the serum. The serum bone-specific alkaline phosphatase (BALP) and tartrate-resistant acid phosphatase 5b (TRACP5b) levels were evaluated using a commercial enzyme-linked immunosorbent assay (ELISA) system according to the manufacturer's protocol (Beijing Bioss Biological Technology Co., LTD., China).…”

Section: Biochemical Analysismentioning

confidence: 99%

“…The serum bone-specific alkaline phosphatase (BALP) and tartrate-resistant acid phosphatase 5b (TRACP5b) levels were evaluated using a commercial enzyme-linked immunosorbent assay (ELISA) system according to the manufacturer's protocol (Beijing Bioss Biological Technology Co., LTD., China). As our previous description [7], the left femur BMD was measured using dual energy X-ray absorptiometry (DEXA; Lunar-DPX-IQ, USA). After measuring BMD, the femurs were wrapped around with wet gauzes soaked in normal saline and stored at -20°C until subsequent biomechanical examination.…”

Section: Biochemical Analysismentioning

confidence: 99%

“…In vitro study has reported that PEMFs were capable of increasing the cellular differentiation in MC3T3-E1 osteoblasts [23]. In vivo studies have shown that [7,8] PEMFs inhibited OVXinduced bone loss and deterioration of bone microarchitecture and strength in rats by promoting the activation of Wnt/b-catenin signaling.…”

Section: Introductionmentioning

confidence: 97%

“…As a safe and noninvasive physiotherapy, pulsed electromagnetic fields (PEMFs) have been suggested as a promising treatment for diabetic osteopenia. It has been reported that PEMFs can increase bone mineral density (BMD) in osteoporosis patients [5], prevent bone loss in ovariectomy-induced osteoporosis rats [6][7][8] and hindlimb-suspended rats [9], improve bone formation [10,11], and inhibit bone resorption [12] in vitro. It has reported that the cellular functions of bone marrow stromal cell [13], osteoblast [14,15], and osteoclast [16,17] are abnormal under diabetic conditions.…”

Section: Introductionmentioning

confidence: 99%

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Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

Zhou

Liao

et al. 2014

Endocrine

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Evidences have shown that pulsed electromagnetic fields (PEMFs) can partially prevent bone loss in streptozotocin (STZ)-induced diabetic rats. However, the precise mechanisms accounting for these favorable effects are unclear. This study aimed to investigate the effects of PEMFs on bone mass and receptor activator of nuclear factor κB ligand (RANKL)/osteoprotegerin (OPG) and Wnt/β-catenin signaling pathway in STZ rats. Thirty 3-month-old Sprague Dawley rats were randomly divided into the following three groups (n = 10): control group (injection of saline vehicle), DM group (injection of STZ), and PEMFs group (injection of STZ + PEMFs exposure). One week following injection of STZ, rats in the PEMFs group were subject to PEMFs stimulus for 40 min/day, 5 days/week, and lasted for 12 weeks. After 12 week intervention, the results showed that PEMFs increased serum bone-specific alkaline phosphatase level and bone mineral density, and inhibited deterioration of bone microarchitecture and strength in STZ rats. Furthermore, PEMFs up-regulated the mRNA expressions of low-density lipoprotein receptor-related protein 5, β-catenin and runt-related gene 2 (Runx2), and down-regulated dickkopf1 in STZ rats. However, mRNA expressions of RANKL and OPG were not affected by PEMFs. PEMFs can prevent the diabetes-induced bone loss and reverse the deterioration of bone microarchitecture and strength by restoring Runx2 expression through regulation of Wnt/β-catenin signaling, regardless of its no glucose lowering effect.

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Section: Biochemical Analysismentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

Zhou

Liao

et al. 2014

Endocrine

Self Cite

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“…The functions of osteoblasts can be regulated by various local and hormonal factors such as Wnt glycoproteins. Canonical Wnt/betacatenin signaling pathway plays an important role in the maintenance of bone mass and bone formation [6]. Wnt ligands binding to the frizzled receptor will lead to accumulation and stabilization of beta-catenin in the cytoplasm [7].…”

Section: Introductionmentioning

confidence: 99%

Epigallocatechin-3-gallate Promotes Osteoblastic Activity in Human Osteoblast-like Cells

Peng¹,

Yu²,

Liu³

2016

Trop. J. Pharm Res

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Accelerated Bone Healing via Electrical Stimulation

Sun,

Xie,

et al. 2024

Advanced Science

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Piezoelectric effect produces an electrical signal when stress is applied to the bone. When the integrity of the bone is destroyed, the biopotential within the defect site is reduced and several physiological responses are initiated to facilitate healing. During the healing of the bone defect, the bioelectric potential returns to normal levels. Treatment of fractures that exceed innate regenerative capacity or exhibit delayed healing requires surgical intervention for bone reconstruction. For bone defects that cannot heal on their own, exogenous electric fields are used to assist in treatment. This paper reviews the effects of exogenous electrical stimulation on bone healing, including osteogenesis, angiogenesis, reduction in inflammation and effects on the peripheral nervous system. This paper also reviews novel electrical stimulation methods, such as small power supplies and nanogenerators, that have emerged in recent years. Finally, the challenges and future trends of using electrical stimulation therapy for accelerating bone healing are discussed.

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Effects of Pulsed Electromagnetic Fields on Bone Mass and Wnt/β-Catenin Signaling Pathway in Ovariectomized Rats

Cited by 76 publications

References 44 publications

Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

Pulsed electromagnetic fields inhibit bone loss in streptozotocin-induced diabetic rats

Epigallocatechin-3-gallate Promotes Osteoblastic Activity in Human Osteoblast-like Cells

Accelerated Bone Healing via Electrical Stimulation

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